Instrumentation

仪器仪表

• 批准号: 8254299
• 负责人: DANIEL D LOEB
• 金额: $ 8.83万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2013-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8254299
• 关键词: Blood capillaries; Contracts; DNA Sequence; Detection; Electrophoresis; Equipment; Floor; Fluorescence Microscopy; Funding; Housing; Human; Incubators; Instruction; Maintenance; Modeling; Molecular Biology; Molecular Genetics; Oncogenic Viruses; Research Infrastructure; Resources; Role; Speed; System; Time; Training; capillary; instrument; instrumentation; member; molecular dynamics; programs

Role of the Instrumentation Core Our Instrumentation Core provides this program some of the instrumentation it needs most. It provides in-house and external maintenance for essential equipment. It provides instruction for new instruments or for those instruments difficult to use. It also provides the infrastructure and the collective resources to purchase the instruments most needed by the program members. The Core now provides six kinds of instruments: high speed (J2-21) and ultracentrifuges (two L8M-80); capillary DMA sequencing machines (Molecular Dynamics MegaBACE 1000); real-time PCR (ABI PRISM 7700 Sequence Detection System and 7900 Real-Time PCR System); Spectrophotometer (Beckman model DU7400) and deconvolving, fluorescence microscopy (Zeiss Axiovert 200M). The ultracentrifuges are located on the seventh and eighth floors of McArdle; the capillary sequencing machines and the ABI 7700 on the second floor; the Spectrophotometer is on the seventh floor; and the Axiovert 200M on the eighth floor. This Core is requesting funds for the maintenance contracts on three items: one ultracentrifuge, one Spectrophotometer, and the ABl 7700. The maintenance of other instrumentation including additional centrifuges, microfuges, electrophoresis equipment, and incubators is provided by Mr. Fechner who trained in McArdle and with the Beckman Company. He has provided our program his expertise since its inception. New users within the program of the Axiovert 200M will be trained and assisted by Stephanie Bartley. Bradley Stewart will train and assist users of the Real-Time PCR and DNA Sequencing.

仪器核心的作用 我们的仪器核心为该程序提供了一些它最需要的仪器。它为重要设备提供内部和外部维护。它为新仪器或那些难以使用的仪器提供了说明。它还提供基础设施和集体资源来购买计划成员最需要的仪器。 Core现提供六种仪器：高速（J2-21）和超速离心机（两台L8M-80）；毛细管 DMA 测序仪（分子动力学 MegaBACE 1000）；实时PCR（ABI PRISM 7700序列检测系统和7900实时PCR系统）；分光光度计（Beckman 型号 DU7400）和去卷积荧光显微镜（Zeiss Axiovert 200M）。超速离心机位于 McArdle 的七楼和八楼；二楼的毛细管测序机和 ABI 7700；分光光度计在七楼；以及八楼的Axiovert 200M。该核心正在申请三项维护合同的资金：一台超速离心机、一台分光光度计和 ABl 7700。其他仪器的维护，包括额外的离心机、微量离心机、电泳设备和培养箱，由在 McArdle 接受培训的 Fechner 先生提供以及贝克曼公司。自我们的项目启动以来，他就为我们提供了他的专业知识。 Axiovert 200M 计划中的新用户将由 Stephanie Bartley 接受培训和协助。 Bradley Stewart 将培训和协助实时 PCR 和 DNA 测序的用户。