Polypeptide Design with Proteomic Scope via Microfluidic mRNA Display

通过微流控 mRNA 显示进行蛋白质组学多肽设计

基本信息

批准号：
8320282
负责人：
RICHARD W ROBERTS
金额：
$ 47.55万
依托单位：
UNIVERSITY OF SOUTHERN CALIFORNIA
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-09-30 至 2014-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8320282
关键词：
Affinity Amino Acid Sequence Amino Acids Antibodies Antibody Affinity Area Binding Biological Biology Capillary Electrophoresis Cell Culture Techniques Cells Chimeric Proteins Chronic Hepatitis Cirrhosis Codon Nucleotides Complement Complementary DNA Complex Cyclic Peptides DNA Data Detection Development Devices Diagnostic Directed Molecular Evolution Discipline Disease Elements Engineering Epitopes Evolution Exhibits Family Fibronectins Flaviviridae Flu virus Genetic Genetic Techniques Genome Genomics Goals HIV Health Hepatitis C Hepatitis C virus Human In Vitro Label Laboratories Libraries Life Life Cycle Stages Link Liquid substance Liver diseases Lysine Medical Messenger RNA Methods Metric Microfluidic Microchips Microfluidics Molecular Non-Hodgkin&apos s Lymphoma Pattern Peptide Hydrolases Peptide Library Peptide Sequence Determination Peptides Phage Display Pharmaceutical Preparations Phase I Clinical Trials Phosphorylation Physics Play Polyproteins Polysaccharides Positioning Attribute Post-Translational Protein Processing Prevention therapy Primary carcinoma of the liver cells Property Protein Binding Protein Chemistry Proteins Proteolytic Processing Proteome Proteomics Puromycin RNA RNA-Directed DNA Polymerase Randomized Reagent Recovery Resistance Resolution Role Route SARS coronavirus Series Sorting - Cell Movement Structure Surface Technology Tertiary Protein Structure The Sun Therapeutic Transfection Translating Translations Vaccines Vascular Endothelial Growth Factors Viral Viral Proteins Virus Virus Replication Work aptamer austin base design disulfide bond experience glycosylation interest magnetic beads member mimetics molecular recognition multidisciplinary new technology novel particle pathogen polypeptide prevent protein function protein misfolding research study response skills three dimensional structure virology virus host interaction

项目摘要

DESCRIPTION (provided by applicant): In this project, we propose to combine mRNA display (a protein design/evolution method) and high efficiency microfluidic sorting to create a new technology-microfluidic mRNA display-for the purpose of enabling design of peptides and proteins that can be used as protein capture reagents. We will develop and apply this powerful new technology toward creating a comprehensive reagent set aimed at the Hepatitis C virus (HCV) proteome. In this section, we begin by describing the existing state-of-the-art in 1) mRNA display-based peptide and protein design, 2) bead-based micromagnetic separations, and 3) give an introduction to the proteins expressed by the HCV that will be the targets of this work. This is followed by our description of how we will integrate these technologies to achieve our goal of high-throughput development of new protein capture reagents.

描述（由申请人提供）：在这个项目中，我们建议将mRNA展示（一种蛋白质设计/进化方法）和高效微流控分选相结合，创建一种新技术——微流控mRNA展示——以实现肽和蛋白质的设计可用作蛋白质捕获试剂。我们将开发并应用这项强大的新技术，以创建针对丙型肝炎病毒（HCV）蛋白质组的综合试剂组。在本节中，我们首先描述 1) 基于 mRNA 展示的肽和蛋白质设计，2) 基于珠子的微磁分离，以及 3) 介绍 HCV 表达的蛋白质。这将是这项工作的目标。接下来是我们将如何整合这些技术来实现我们高通量开发新型蛋白质捕获试剂的目标的描述。