Nanion Syncro Patch 96

Nanion 同步补丁 96

• 批准号: 8334952
• 负责人: ROBERT S KASS
• 金额: $ 91.39万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-20 至 2013-07-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8334952
• 关键词: Academic Medical Centers; Amyotrophic Lateral Sclerosis; Area; Arrhythmia; Bathing; Calcium; Cardiac Myocytes; Cells; Collaborations; Data; Derivation procedure; Disease; Dose; Equipment; Funding; Heart failure; Human; Individual; Laboratories; Measurement; Measures; Motor Neurons; Neurons; New York; New York City; Osteoblasts; Patients; Pharmaceutical Preparations; Phenotype; Physiological; Potassium Channel; Property; Regenerative Medicine; Research; Research Personnel; Research Project Grants; Screening procedure; Seasons; Skeletal Myoblasts; Smooth Muscle; Solutions; Source; Speed; Stem cells; Stretching; System; Technology; Time; United States National Institutes of Health; Universities; biological research; channel blockers; embryonic stem cell; high throughput screening; induced pluripotent stem cell; instrument; metropolitan; novel; patch clamp; research study; response; seal; small molecule; voltage

DESCRIPTION (provided by applicant): Electrophysiological studies of electrically excitable cells, such as cardiac myocytes, skeletal myoblasts, neurons, osteoblasts, are critically important for the derivation of these cells from various stem cell sources and for their applicatio in regenerative medicine, fundamental biological research, and study of disease. For example, only electrophysiological measurements can determine if functional cardiomyocytes and neurons have been generated, as marker expression is necessary but not sufficient for the establishment of cell phenotype. These measurements need to be done at high speed (short time constants for changes of bath solutions) and in high-throughput fashion to provide realistic information about the cell properties in a large parameter space. This request is to purchase a SyncroPatch 96 (Nanion Technologies), an automated giga- seal patch clamp (APC) system for studies of electrophysiological properties of single cells with the highest throughput presently available. This core instrument would provide advanced technical capabilities to seven diverse NIH-funded laboratories across both campuses of Columbia University, by rapidly generating high quality patch clamp data for accurate pharmacological and physiological characterization of electrically excitable cells. Full dose response curves are rapidly measured in individual cells with a fast exchange of the bath solutions (within 100 ms), in a 96-well plate format. The system would provide unique capabilities not presently available either at the Columbia University Medical Center, the Columbia University Morningside Campus, or at any other University in the New York Metropolitan area. As detailed in the descriptions of the individual research projects in Section B, the SyncroPatch 96 would greatly accelerate our ongoing research in seven different areas: (i) High throughput screening of iPS-cardiomyocytes derived from patients carrying heritable arrhythmias, (ii) Determination of the impact of pacing and stretch on the maturation of cardiac myocytes derived from human iPS and embryonic stem cells (hESCs), (iii) Screening for novel drugs to treat intracellular calcium leak in heart failure, (iv) Modulation of voltage-dependent potassium channels; (v) Determination of the structural inter-subunit interface in a key smooth muscle potassium channel, (vi) Identification of new genetically encoded Ca channel blockers, and (vii) Novel therapies for amyotrophic lateral sclerosis (ALS) through small molecule screens of patient-derived iPS cell motor neurons (iPS-MNs). Preliminary data were collected using the SyncroPatch 96 in all five areas of proposed research. All Major Users of this equipment are seasoned NIH PIs with extensive expertise in analysis of cellular responses. We expect the instrument to be used at its full capacity, by investigators throughout the Columbia University and collaborators in the New York City area, promoting collaboration.

描述（由申请人提供）：对电气可激发细胞的电生理研究，例如心肌细胞，骨骼肌细胞，神经元，成骨细胞，对于从各种干细胞来源衍生而来的这些细胞至关重要，对于它们在再生医学，根本生物学研究和疾病中的应用中的应用。例如，只有电生理测量值才能确定是否已经产生了功能性心肌细胞和神经元，因为标记表达是必要的，但不足以建立细胞表型。这些测量需要高速（用于更改浴室溶液的短时常数）和高通量方式，以提供有关大参数空间中单元特性的现实信息。该请求是购买Syncropatch 96（Nanion Technologies），这是一种自动化的千兆贴片夹（APC）系统，以研究目前可用的最高吞吐量的单个细胞的电生理特性。该核心工具将通过快速生成高质量的贴片夹数据，以准确地对电兴奋的细胞进行准确的药理和生理表征，从而为哥伦比亚大学两个校园的七个不同的NIH资助实验室提供高级技术能力。全剂量响应曲线以96孔板格式在单个细胞中迅速测量（在100毫秒内）。该系统将在哥伦比亚大学医学中心，哥伦比亚大学Morningside校园或纽约大都市地区的任何其他大学提供目前提供的独特功能。 As detailed in the descriptions of the individual research projects in Section B, the SyncroPatch 96 would greatly accelerate our ongoing research in seven different areas: (i) High throughput screening of iPS-cardiomyocytes derived from patients carrying heritable arrhythmias, (ii) Determination of the impact of pacing and stretch on the maturation of cardiac myocytes derived from human iPS and embryonic stem cells (hESCs), （iii）筛查新型药物以治疗心力衰竭中细胞内钙泄漏，（iv）电压依赖性钾通道的调节； （v）确定关键平滑肌钾通道中的结构增生间界面，（VI）鉴定新的遗传编码的CA通道阻滞剂，以及（VII）通过患者衍生的IPS细胞运动神经元的小分子筛选肌萎缩性侧面硬化症（ALS）的新型疗法。在拟议的研究的所有五个领域中，使用Syncropatch 96收集了初步数据。该设备的所有主要用户都是经验丰富的NIH PI，在分析细胞响应方面具有广泛的专业知识。我们希望该工具能够全部使用，由哥伦比亚大学的调查人员和纽约市地区的合作者进行，以促进合作。