ShEEP Request for Particle Matrix ZetaView

ShEEP 请求粒子矩阵 ZetaView

• 批准号: 10741098
• 负责人: Lynn PULLIAM
• 金额: --
• 依托单位: VETERANS AFFAIRS MED CTR SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-03-01 至 2023-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10741098
• 关键词: 8 year old; Affect; Amyotrophic Lateral Sclerosis; Antibodies; Area; Biological; Biological Markers; Carbon Nanotubes; Cardiovascular Diseases; Charge; Communities; Computer software; Diabetes Mellitus; Disease; Dyes; Equipment; Europe; Explosion; Fluorescence; Frontotemporal Dementia; Funding; Future; Goals; HIV; Health; Homeostasis; Inflammation; Label; Lasers; Lighting; Lipids; Liposomes; Malignant Neoplasms; Measures; Methods; Nerve Degeneration; Particle Size; Pathologic; Performance; Polymers; Process; Research; Sampling; San Francisco; Sheep; Surface; Suspensions; System; Techniques; Technology; Thinking; Time; Vesicle; Veterans; Virus; exosome; extracellular vesicles; instrument; nanoparticle; new technology; particle; programs; protein aggregation; user-friendly

Extracellular vesicle (EV) research is a fast-moving area of research. Biological nanoparticles such as EVs, exosomes, viruses, lipids are part of normal homeostasis and now we believe a major part of pathological conditions. The San Francisco VA was the first in the UCSF community to purchase a NanoSight nanoparticle tracking system, 8 years ago. The field has moved very quickly and the new Particle Matrix ZetaView, we believe has replaced the NanoSight technology. The ZetaView can rapidly characterize particles as to size, particle concentration and surface charge. It also has the advantage of characterizing particles by fluorescent labelling without any additives and with a very small sample. The ZetaView has 4 lasers to enable the user to use different dyes to discriminate different particles and allows the user to quantify colocalization ratios of biomarkers. This nanoparticle tracking analysis is rapid and user friendly. This basic characterization of EV particles is essential in any project to use EVs as biomarkers and treatments. This new machine will attract new users to the field of EVS and facilitate current users for their funded projects.

细胞外囊泡（EV）研究是一个快速发展的研究领域。生物纳米粒子 例如 EV、外泌体、病毒、脂质是正常体内平衡的一部分，现在我们相信 病理状况的主要部分。旧金山 VA 是 UCSF 社区中的第一个 8 年前购买 NanoSight 纳米颗粒跟踪系统。田野已经发生了很大的变化 快速和新的粒子矩阵 ZetaView，我们相信已经取代了 NanoSight 技术。 ZetaView 可以快速表征颗粒的尺寸、颗粒浓度和表面 收费。它还具有通过荧光标记来表征颗粒的优点，无需任何 添加剂和非常小的样品。 ZetaView 有 4 个激光器供用户使用 不同的染料来区分不同的颗粒并允许用户量化共定位比率 的生物标志物。这种纳米颗粒跟踪分析快速且用户友好。这个基本的 在任何使用 EV 作为生物标记物的项目中，EV 颗粒的表征都是至关重要的 治疗。这款新机将吸引新用户进入EVS领域并方便现有用户 为他们资助的项目。