Tracking stem cells in engineered tissue and organs in vivo and in real time

体内实时追踪工程组织和器官中的干细胞

• 批准号: 8319267
• 负责人: JEREMY J MAO
• 金额: $ 54.78万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-15 至 2014-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8319267
• 关键词: Adherent Culture; Adipocytes; Apoptosis; Arthritis; Back; Behavior; Biocompatible Materials; Biological Assay; Biomedical Engineering; Cardiac; Cell Proliferation; Cell Survival; Cell Transplants; Cells; Chondrocytes; Data; Defect; Development; Developmental Biology; Dyes; Encapsulated; Engineering; Goals; Healed; Home environment; Human; Human Activities; Hydrogels; Image; Implant; In Vitro; Joints; Label; Lead; Ligaments; Mechanics; Mesenchymal Stem Cells; Metabolism; Methods; Microscopic; Modality; Molecular; Mus; Muscle; Musculoskeletal; Natural regeneration; Neurons; Nude Rats; Organ; Osteoblasts; Outcome Assessment; Pancreas; Photobleaching; Population; Proliferating; Proteins; Quantum Dots; Rattus; Regenerative Medicine; Relative (related person); SCID Mice; Signal Transduction; Stem cells; System; Tail; Technology; Tendon structure; Testing; Time; Tissue Engineering; Tissues; Veins; Wound Healing; bone; cytotoxicity; healing; improved; in vivo; osteochondral tissue; public health relevance; response; scaffold; tissue regeneration; tool

DESCRIPTION (provided by applicant): In response to PAR-06-504 "Enabling Technologies for Tissue Engineering and Regenerative Medicine," we present an enabling technology of tracking stem cells with bioconjugated quantum dots (QDs) by non-destructive and non- invasive imaging, both in vivo and in real time. A common challenge in tissue regeneration, similar to developmental biology, is the tracking of cells and tissues that are developing in real time. Our preliminary data demonstrate that bioconjugated QDs are safe to label human mesenchymal stem cells (hMSCs) during proliferation for several passages, as well as differentiation into chondrocytes and osteoblasts. We also show that QD-labeled hMSCs, injected via the tail vein of SCID mice, can be tracked in vivo and in real-time by a whole body imager system. Furthermore, we were able to track multiplexing QD- labeled hMSCs injected subcutaneously in vivo and in real time. Theses findings lead to our overall hypothesis that stem cells delivered systemically home to local defects and participate in defect healing, which has been difficult to study previously due to the lack of in vivo and real time cell tracking modalities. The overall goal of this proposal is to determine the efficacy of QD labeling and tracking of MSCs in vivo and in real time, and to improve our understanding of the relative contribution of different cell populations to the healing of tissue defect. Our long-term goal is to apply QD labeling for cell tracking as a platform technology for the healing of other tissues such as cardiac, neuronal, and pancreatic, as well as other musculoskeletal tissues such as bone-ligament interface, muscle-tendon junction, etc. Public Health Relevance Statement (provided by applicant): This project has the potential to demonstrate a viable method for labeling stem cells and tracking the development of regenerating tissue in vivo and in real time. It also has a potential to contribute to our understanding of the engineered osteochondral interface that is of critical value in bioengineered replacement of arthritic joints.

描述（由申请人提供）： 为了响应Par-06-504“组织工程和再生医学的能力技术”，我们提出了一种能够通过在体内和实时和实时和实时和实时和实时和实时的，可以通过非破坏性和非侵入性成像跟踪具有生物偶联量子点（QD）的干细胞技术。与发育生物学类似的组织再生中的一个普遍挑战是实时发展的细胞和组织。我们的初步数据表明，生物偶联的QD在增殖过程中可以安全地标记人间充质干细胞（HMSC），并分化为软骨细胞和骨细胞的分化。我们还表明，可以通过整个身体成像器系统实时跟踪通过SCID小鼠的尾静脉注射的QD标记的HMSC。此外，我们能够在体内和实时跟踪地下注射的QD QD标记的HMSC。这些发现导致了我们的总体假设，即干细胞从系统地寄回了局部缺陷并参与缺陷愈合，这由于体内缺乏体内和实时细胞跟踪方式，因此很难先前研究。该提案的总体目标是确定QD标记和MSC在体内和实时实时跟踪的功效，并提高我们对不同细胞群体对组织缺陷愈合的相对贡献的理解。我们的长期目标是将QD标记应用于细胞跟踪，作为一种平台技术，以治愈其他组织，例如心脏，神经元和胰腺，以及其他肌肉骨骼组织，例如骨凸界，肌肉 - 肌肉 - 肌肉 - 肌肉 - 肌肉 - 肌肉 - 肌肉交界器等。 公共卫生相关性声明（由申请人提供）：该项目有可能证明一种可行的方法来标记干细胞并实时跟踪重生组织的发展。它还有可能有助于我们对工程学的骨软骨界面的理解，这在关节炎关节的生物工程替换中具有关键价值。

项目成果

Regenerative endodontics: barriers and strategies for clinical translation.

• DOI: 10.1016/j.cden.2012.05.005
• 发表时间: 2012-07
• 期刊: Dental clinics of North America
• 作者: Mao JJ;Kim SG;Zhou J;Ye L;Cho S;Suzuki T;Fu SY;Yang R;Zhou X
• 通讯作者: Zhou X

Musculoskeletal tissue engineering by endogenous stem/progenitor cells.

内源干/祖细胞的肌肉骨骼组织工程。

• DOI: 10.1007/s00441-012-1339-2
• 发表时间: 2012
• 期刊: Cell and tissue research
• 影响因子: 3.6
• 作者: Nie,Hemin;Lee,ChangHun;Tan,Jiali;Lu,Chuanyong;Mendelson,Avital;Chen,Mo;Embree,MildredC;Kong,Kimi;Shah,Bhranti;Wang,Shuang;Cho,Shoko;Mao,JeremyJ
• 通讯作者: Mao,JeremyJ