Tissue and Pathology Resources

组织和病理学资源

基本信息

批准号：
8327257
负责人：
Massimo Loda
金额：
$ 19.76万
依托单位：
DANA-FARBER CANCER INST
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至 2013-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8327257
关键词：
Algorithms Anatomy Antibodies Apoptosis Area Bacterial Artificial Chromosomes Bioinformatics Biological Biological Assay Biometry Biopsy Biopsy Specimen Biostatistics Core Blood Blood specimen CD34 gene Cancer cell line Cathepsins B Cell Proliferation Cells Cellularity Cetuximab Chromosome abnormality Chromosomes, Human, Pair 4 Clinical Clinical Data Collaborations Collection Colon Colon Carcinoma Colonic Adenoma Colonic Neoplasms Color Colorectal Cancer Complex Consent Consultations Dana-Farber Cancer Institute Data Databases Development Diagnostic Diagnostic Neoplasm Staging Disease Dissection Emerging Technologies Enrollment Ensure Environment Erythrocytes Evaluation Fine needle aspiration biopsy Fluorescein-5-isothiocyanate Formalin Fostering Freezing Fresh Tissue Funding Future Gastrointestinal Neoplasms Gene Expression Profiling Genes Genetically Engineered Mouse Genomics Goals Heterogeneity Histologic Histology Human Image Image Analysis Imaging technology Imatinib Immunohistochemistry In Situ Hybridization Individual Institution Institutional Review Boards Investigation Label Lesion Lesion by Morphology Link Liquid substance Lymph Node Dissections Lymph Node Tissue Malignant Neoplasms Malignant neoplasm of gastrointestinal tract Malignant neoplasm of pancreas Maps Methods Microdissection Microsatellite Instability Microscopic Modeling Molecular Molecular Analysis Molecular Profiling Molecular Target Monitor Monoclonal Antibodies Mucous Membrane Mus Neoplasm Metastasis Neoplasms Nitrogen Nuclear Oligonucleotides Operative Surgical Procedures PDGFRA gene PECAM1 gene Pancreas Pancreatic Adenocarcinoma Paraffin Paraffin Embedding Paraffin Tissue Pathologic Pathologist Pathology Patients Performance Peripheral Blood Mononuclear Cell Pharmaceutical Preparations Phospho-Specific Antibodies Plasma Population Positioning Attribute Principal Investigator Process Proteins Proteomics Protocols documentation Publications Quality Control Quantum Dots RNA Research Research Infrastructure Research Personnel Research Project Grants Resected Resistance Resources Rhodamine Sampling Screening procedure Services Signal Pathway Signal Transduction Site Slide Specimen Staging Staining method Stains Supervision Surgeon System Systems Analysis Technical Expertise Technology Testing Therapeutic Time Tissue Banking Tissue Banks Tissue Embedding Tissue Harvesting Tissue Microarray Tissue Sample Tissues Tracer Translating Tumor stage Validation Vitamin D Western Blotting Work adenoma base cancer genomics carcinogenesis caspase-3 cellular imaging cohort colon dysplasia cryostat flexibility gastrointestinal gene discovery human tissue improved indexing innovation laser capture microdissection lymph nodes macrophage meetings metastatic colorectal molecular imaging molecular pathology molecular/cellular imaging mouse model nanoparticle neoplastic new technology novel novel strategies pancreatic neoplasm prognostic programs protein expression radiologist repository research study sample fixation success tool treatment duration tumor tumor progression

项目摘要

The pathologic analysis and molecular characterization of human tissue samples is a fundamental and integral requirement for all portions of this SPORE application, including systematic pathologic evaluation of micrometastatic disease, collection and analysis of human tissue samples for oncogenomics and gene discovery, evaluation of genetically engineered mouse neoplasms with moleclular imaging correlates, analysis of cancer signaling pathways, and characterization of tumoral heterogeneity in the emergence of chemoresistance. The core is highly integrated with each of the 5 major projects. We will work in close collaboration with the Project Investigators for three specific purposes: i) to provide tissue specimens, histology services and standardized systematic morphologic consultative expertise in the pathologic evaluation of human gastrointestinal neoplasms; ii) to provide infrastructure and technical expertise for a variety of molecular pathologic assays, including high-efficiency screening and validation of genomic and proteomic targets in human gastrointestinal tissue specimens; and iii) to evaluate and implement new cellular imaging and analysis technologies that will greatly facilitate these research goals in future. Centralization of these Core activities builds upon the established infrastructure and intellectual expertise of the investigators, and provides a highly valuable component to the analysis of biological resources developed and utilized by project investigators. In addition, the core has strong integrations with the Biostatistics Core and Genomic Data and Bioinformatics Cores (Cores 3 and 4), thereby leveraging the greatest benefits from these resources, and providing a wealth of opportunities for significant advances in understanding of gastrointestinal carcinogenesis. Our specific aims are as follows: (1) to provide tissue specimens, histologic processing services and pathologic analysis of human gastrointstinal neoplasms; (2) to characterize human gastrointestinal neoplasms using molecular pathology tools; (3) evaluate and implement new technologies for cellular imaging and molecular characterization.

人体组织样本的病理分析和分子表征是一种基本，该孢子应用的所有部分的整体要求，包括系统的病理评估人体组织样品的微分转移性疾病和分析致癌基因和基因发现，评估具有分子成像的基因工程小鼠肿瘤的相关性，分析癌症信号通路和肿瘤异质性的表征化学抗性。核心与5个主要项目中的每个项目都高度融合。我们将近距离工作与项目调查人员合作出于三个特定目的：i）提供组织标本，组织学服务和病理学的标准化系统形态咨询专业知识人类胃肠道肿瘤的评估； ii）提供基础架构和技术专长各种分子病理测定，包括高效筛查和基因组和验证人胃肠道试样中的蛋白质组学靶标； iii）评估和实施新的蜂窝成像和分析技术将在将来极大地促进这些研究目标。集中化这些核心活动基于研究人员的既定基础设施和知识专业知识并为分析生物资源的分析提供了非常有价值的组成部分项目调查人员。此外，核心与生物统计学核心和基因组具有很强的整合数据和生物信息学核心（核3和4），从而利用这些核心最大的好处资源，并为理解的重大进展提供了丰富的机会胃肠道致癌。我们的具体目的如下：（1）提供组织标本，组织学处理服务和人类胃肠道肿瘤的病理分析；（2）表征人类胃肠道使用分子病理工具的肿瘤；（3）评估和实施用于蜂窝的新技术成像和分子表征。