Multiscale, Multimodal Analysis of Skin and Spatial Cell Organization

皮肤和空间细胞组织的多尺度、多模式分析

• 批准号: 10826224
• 负责人: Fiona Ginty
• 金额: $ 15万
• 依托单位: GENERAL ELECTRIC GLOBAL RESEARCH CTR
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-22 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10826224
• 关键词: 3-Dimensional; Adoption; Algorithms; Anatomy; Antibodies; Arteries; Biological Markers; Blood; Blood Vessels; Cell physiology; Cells; Classification; Collaborations; Communities; Data; Data Analyses; Endothelial Cells; Goals; Guidelines; Human BioMolecular Atlas Program; Imaging technology; Immune; Liquid substance; Location; Lymph; Lymphatic; Lymphatic Capillaries; Lymphocyte; Maps; Organ; Oxygen; Regulation; Resources; Sampling; Skin; Stains; Structure; Tissues; Vasodilation; Veins; Visualization; Work; cell type; collaboratory; community building; lymphatic vessel; multimodality; multiplexed imaging; nutrition; reconstruction; tissue mapping; working group

Project Summary/Abstract Tissue vasculature and lymphatics are an essential component of every organ and transport blood and lymph around the body, performing essential functions such as vasodilation regulation and immune cell transport. The goal of this supplemental proposal is to collaborate with the HuBMAP ZIPCODE project to develop a panel of antibodies that are specific for vascular and lymphatic cells and structures. We will provide this as a resource for the HuBMAP community for more comprehensive endothelial and lymphatic cell mapping across HuBMAP tissue mapping centers. We will generate data by staining those antibodies in skin using two multiplexed imaging technologies that are currently in use in HuBMAP (CODEX and Cell DIVE), using sequentially sectioned skin samples. We will use existing algorithms for 2D cell classification and 3D volume reconstruction of vascular and lymphatic cell types in these sequential sections. This data will be used as a resource by the HIVE and broader HuBMAP community for building maps of vascular and lymphatics vessels. Together with the ZIPCODE work, our project will help guide efforts across HuBMAP to triangulate distance between the closest artery, vein, and lymphatic capillary and relationships with nearest immune cells and other cell types. We will work with the ZIPCODE team to develop marker sets, best practices, and guidelines to enable efficient alignment and adoption across the Tissue Mapping Centers (TMCs) and activities in HuBMAP. To achieve this, we will work as a collaborative team to coordinate with the Anatomical Structures, Cell Types, plus Biomarkers (ASCT+B) tables, Organ Mapping Antibody Panels (OMAPs), data analysis working group, and HuBMAP Integration, Visualization & Engagement (HIVE) Collaboratory. We envisage that this granular mapping of endothelial cells and lymphatics will help incorporate cellular ZIP codes in spatial tissue profiling, i.e., connecting cellular function to the triangular distance between the closest artery, vein, and lymphatic capillary and provide new data on spatial location and functions of neighboring cells.

项目概要/摘要 组织脉管系统和淋巴管是每个器官和运输血液的重要组成部分 和身体周围的淋巴，执行基本功能，例如血管舒张调节和 免疫细胞运输。该补充提案的目标是与 HuBMAP 合作 ZIPCODE 项目将开发一组针对血管和淋巴细胞的特异性抗体 和结构。我们将将此作为资源提供给 HuBMAP 社区以获取更多信息 HuBMAP 组织图谱中心的全面内皮细胞和淋巴细胞图谱。 我们将使用两种多重成像对皮肤中的这些抗体进行染色来生成数据 目前在 HuBMAP（CODEX 和 Cell DIVE）中使用的技术，按顺序使用 切片皮肤样本。我们将使用现有算法进行 2D 细胞分类和 3D 体积 在这些连续切片中重建血管和淋巴细胞类型。该数据将是 HIVE 和更广泛的 HuBMAP 社区将其用作构建血管地图的资源 和淋巴管。我们的项目将与邮政编码工作一起帮助指导工作 穿过 HuBMAP，对最近的动脉、静脉和毛细淋巴管之间的距离进行三角测量 以及与最近的免疫细胞和其他细胞类型的关系。我们将与邮政编码合作 团队开发标记集、最佳实践和指南，以实现高效对齐和 组织作图中心 (TMC) 和 HuBMAP 活动中的采用。为了实现这一目标， 我们将作为一个协作团队来协调解剖结构、细胞类型、 加上生物标志物 (ASCT+B) 表、器官图谱抗体组合 (OMAP)、数据分析 工作组以及 HuBMAP 集成、可视化和参与 (HIVE) 协作实验室。 我们设想这种内皮细胞和淋巴管的颗粒图谱将有助于整合 空间组织分析中的细胞邮政编码，即将细胞功能与三角形连接起来 最近的动脉、静脉和毛细淋巴管之间的距离，并提供空间方面的新数据 相邻小区的位置和功能。