Transgenic Mouse Core

转基因小鼠核心

基本信息

批准号：
7810378
负责人：
ANNE E GRIEP
金额：
$ 30.29万
依托单位：
INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-09-15 至 2015-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7810378
关键词：
Address Alleles Animal Care and Use Committees Animal Model Animals Applications Grants Autoimmunity Biotechnology Breeding Bronchiolitis Cardiac Cessation of life Chest Chimera organism Chronic Clinical Collagen Collagen Type V Communities Coronary Credentialing Deposition Disease Extracellular Matrix Fee-for-Service Plans Gene Targeting Generations Genetic Genetic Identity Genetically Engineered Mouse Genotype Goals Health Services Research Heart Heart Transplantation Heel Housing Human Resources Indiana Individual Institutes Institution Knockout Mice Laboratory Technicians Link Lung Lung Transplantation Maintenance Medical Research Microinjections Mouse Strains Mus Mutant Strains Mice Organ Transplantation Pathway interactions Play Process Production Program Research Project Grants Protocols documentation Public Health Rattus Research Resources Rodent Role Services Specific Pathogen Frees Technical Expertise Tissues Training Transgenic Animals Transgenic Mice Transgenic Organisms Transplant Recipients Transplantation Universities Vascular Diseases Waiting Lists Wisconsin Work animal breeding animal facility base blastocyst embryonic stem cell experience germ free condition heart allograft medical schools mutant novel programs research study response

项目摘要

The overall goal of this Program Project grant application is to elucidate the mechanisms of autoimmunity to type V collagen in heart and lung transplants. The use of animal models to study mechanism is essential for advancing our understanding of this important clinical problem. To this end, the focus of the Transgenic Mouse Core (Core B) is to provide the technical expertise for the generation of genetically modified mouse strains and to maintain a colony of these unique mouse strains such that mice are generated for the experiments that address the aims of the proposed three Projects. To do so, the first part of Core B will utilize personnel in the University of Wisconsin Biotechnology Center's (UWBC) Transgenic Animal Facility (TAF) to (1) carry out gene targeting experiments in embryonic stem cells to produce ES cell clones with genetically altered alleles and the blastocyst microinjections to generate new mouse mutant strains carrying these mutant alleles, (2) to rederive genetically modified strains that are obtained from other institutions or are housed presently in non-SPF animal facilities so that the mouse stocks for this project can be housed in a newly opened specific pathogen free vivarium at the University of Wisconsin, and (3) cryopreserve for long term storage and safe keeping all the genetically modified mouse strains used in this proposal. In the second part of Core B, Core staff will (1) verify the genetic identity of all the mutant mouse strains rederived for the projects and the inducible tissue specific expression of certain strains, (2) maintain a breeding colony of all the genetically modified mouse strains such that the required numbers of the mouse stocks can be supplied to the individual Projects as needed for their experimentation. This Core will provide specific services for each of the three projects in this proposal. The services provided by this Core will be essential for achieving the overall goals of this grant application.

该计划项目拨款申请的总体目标是阐明自身免疫机制 V型胶原蛋白在心脏和肺移植中的应用。使用动物模型来研究机制至关重要增进我们对这一重要临床问题的理解。为此，转基因的重点 Mouse Core（Core B）是为转基因小鼠的产生提供技术专长品系并维持这些独特的小鼠品系的群体，以便产生小鼠解决拟议三个项目目标的实验。为此，核心 B 的第一部分将利用威斯康星大学生物技术中心 (UWBC) 转基因动物设施的人员 (TAF)至(1)在胚胎干细胞中进行基因打靶实验，以产生ES细胞克隆基因改变的等位基因和囊胚显微注射产生新的小鼠突变株，携带这些突变等位基因，(2) 重新衍生从其他机构获得的转基因菌株或目前饲养在非 SPF 动物设施中，因此该项目的小鼠种群可以饲养在威斯康星大学新开设的无特定病原体动物饲养场，以及（3）长期冷冻保存长期储存并安全保存本提案中使用的所有转基因小鼠品系。在第二个作为核心 B 的一部分，核心工作人员将 (1) 验证所有重新衍生的突变小鼠品系的遗传身份项目和某些菌株的诱导组织特异性表达，（2）维持所有菌株的繁殖群体转基因小鼠品系，以便可以提供所需数量的小鼠种群根据实验需要分配给各个项目。该核心将为以下人员提供特定服务：该提案中的三个项目中的每一个。该核心提供的服务对于实现本次拨款申请的总体目标。