Transgenic and Mutant Animals

转基因和突变动物

基本信息

批准号：
7491894
负责人：
ANNE E GRIEP
金额：
$ 14.62万
依托单位：
UNIVERSITY OF WISCONSIN-MADISON
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-07-23 至 2012-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7491894
关键词：
Animal Husbandry Animals Biotechnology Breeding Communities Consult Cryopreservation DNA Embryo Engineering Equation Equipment Experimental Designs Female Generations Genomics Human Resources Injection of therapeutic agent Knockout Mice Laboratories Malignant Neoplasms Manuals Microinjections Micromanipulation Microscopic Mission Molecular Biology Molecular Genetics Mus Mutate Nature Ovarian Partner in relationship Preparation Prevention Procedures Process Production Protocols documentation Rattus Reproductive Biology Research Resources Services Southern Blotting Tail Techniques Technology Time Today Training Transgenic Animals Transgenic Mice Transgenic Organisms Transplantation Universities University of Wisconsin Comprehensive Cancer Center Wisconsin animal breeding animal facility blastocyst embryo surgery embryonic stem cell experience in vivo Model insight investigator training member mutant reproductive research study skills success vector

项目摘要

Transgenic and genetically-engineered mutant animals provide one of the most valuable mechanisms for generating in vivo models for unraveling the molecular and genetic mechanisms of cancer and for evaluating new strategies for treatment and prevention studies. Efficient generation of transgenic animals requires great technical skill that is acquired only after extensive experience with animal husbandry and breeding, animal surgery, embryo manipulation and micromanipulation. The transgenic Animal Shared Service makes transgenic technology accessible and affordable to the members.of the University of Wisconsin Comprehensive Cancer Center. It uses the personnel, facilities and equipment of the campus-wide University of Wisconsin Biotechnology Center's Transgenic Animal Facility. Services currently provided by the Transgenic Animals Core include: Generation of transgenic mice and rats by pronuclear microinjection Cryopreservation of mouse and rat embryos ¿ Generation of knockout mice: generation of targeting vectors, generation of targeted embryonic stem (ES) cell clones, and generation of chimeric mice by injection of ES cells into host blastocyst Strain rederivations and ovarian transplants Other miscellaneous molecular biology and embryo services related to transgenic and knockout mouse production Consulting and training In addition, the staff are available for consulting at any point in the process of using the services. Considerable time is spent discussing experimental design and providing guidance on processes that they themselves are or will "be carrying out. They also train investigators in techniques such as superovulating, mating, and collecting reproductive tracts from female mice for embryo cryopreservation, isolating DMA for pronuclear micronjections, mouse handling and breeding protocols, and cutting tails and genomic DNA preparation Southern blotting to identify correctly mutated ES cells.

转基因和基因工程突变动物提供了最有价值的机制之一生成体内模型以揭示癌症的分子和遗传机制并进行评估治疗和预防研究的新策略需要大量的转基因动物的产生。只有经过丰富的畜牧业和养殖业经验后才能获得的技术技能转基因动物共享服务使手术、胚胎操作和显微操作成为可能。威斯康星大学成员可以使用并负担得起转基因技术综合癌症中心使用全校的人员、设施和设备。威斯康星大学生物技术中心的转基因动物设施服务目前由其提供。转基因动物核心包括：原核显微注射产生转基因小鼠和大鼠小鼠和大鼠胚胎的冷冻保存 ¿ 基因敲除小鼠的产生：靶向载体的产生、靶向胚胎干（ES）的产生细胞克隆，以及通过将 ES 细胞注射到宿主囊胚中产生嵌合小鼠菌株再衍生和卵巢移植与转基因和基因敲除小鼠相关的其他杂项分子生物学和胚胎服务生产咨询与培训此外，在使用服务的过程中，工作人员可以随时提供咨询。花费大量时间讨论实验设计并为他们的流程提供指导他们自己正在或将会“正在执行。他们还对调查人员进行超数排卵、交配，收集雌性小鼠的生殖道用于胚胎冷冻保存，分离DMA用于原核显微注射、小鼠处理和育种方案以及切割尾巴和基因组 DNA 准备Southern blotting以识别正确突变的ES细胞。