Molecular and Anatomic Imaging Core

分子和解剖成像核心

• 批准号: 8344385
• 负责人: Boyce Brendan
• 金额: $ 36.33万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8344385
• 关键词: Algorithms; Allografting; Anatomy; Animal Model; Animals; Basic Science; Biological Assay; Bone Diseases; Bone callus; Canis familiaris; Cartilage; Clinical; Clinical Sciences; Communication; Computer software; Data; Development; Disease; Enrollment; Ensure; Fracture; Funding; Gene Expression; Goals; Healed; Histologic; Human; Human Resources; Image; Image Analysis; Imaging Techniques; Imaging technology; Immunohistochemistry; In Situ Hybridization; Laboratories; Methodology; Modeling; Molecular; Morphologic artifacts; Morphology; Mus; Muscle; Musculoskeletal; Musculoskeletal Diseases; Orthopedics; Osseointegration; Pilot Projects; Policies; Pre-Clinical Model; Procedures; Process; Quality Control; Research; Research Personnel; Research Project Grants; Sampling; Schedule; Services; Signal Pathway; Signal Transduction; Slide; Structure; System; Techniques; Technology; Teriparatide; Tissue Microarray; Tissue Sample; Tissues; Translating; Translational Research; Translations; Work; X-Ray Computed Tomography; base; bone; bone loss; clinical application; clinical practice; cone-beam computed tomography; cost; cost effective; healing; human disease; human subject; human tissue; imaging modality; implantation; improved; innovation; meetings; molecular imaging; new technology; novel; protein expression; research study; response; software systems; trauma care

The Molecular and Anatomic Imaging Core will provide molecular and anatomic imaging services to support the clinical and basic science components of all 3 of the projects under a single administrative structure. In all of the projects there is a significant emphasis on the correlation of histologic and cellular findings with various anatomic imaging modalities. By combining these services, the Core can not only support translation in the sense of basic science to clinical application, but also enable translation between molecular and anatomic imaging techniques among the Projects, which can enhance both components of the CORT. In addition, the Core will develop new techniques to enhance the analysis of changes induced in bone and muscles by teriparatide in the various animals studies. The overall Goals of the Core are to continue to: A. provide high quality, efficient, and cost-effective tissue-based molecular imaging studies; B. provide high quality, consistent, efficient and cost-effective anatomic imaging studies in animals; and C. enhance and refine technology to bring new cutting-edge imaging techniques into basic research practice. The Specific Alms related to Goal C are to: 1. Develop Whole Slide Imaging technology using Olympic's VS100 whole slide imager to capture histologic and other digitally-acquired images for automated quantitative analysis of standard and novel parameters of tissue morphology; 2. Develop quantitative methodology to evaluate signaling using immunohistochemistry (IHC) and in-situ hybridization (ISH) and the VS100 slide imager in tissue samples of cartilage and fracture callus; 3. Continue to develop Tissue Microarrays (TMAs) of murine tissues to optimize IHC analysis of protein expression and quantify expression automatically using the whole slide imager; 4. Optimize algorithms for hardware artifact suppression and calculating the Union Ratio from clinical Cone Beam CT (CB-CT) scanning to aid in the assessment of osseointegration of allografts in the large animal (canine) preclinical model. This translation between molecular and anatomic imaging will be facilitated by an Imaging Core Committee, which will oversee the day to day working of the two components of the Core and assist in the development of new technologies to support the projects.

分子和解剖成像核心将提供分子和解剖成像服务以支持 在单个行政结构下，所有三个项目的临床和基础科学组成部分。在 所有的项目都非常重视组织学和细胞发现与 各种解剖成像方式。通过结合这些服务，核心不仅可以支持翻译 从临床应用的基础科学意义上，也可以在分子和 项目之间的解剖成像技术可以增强Cort的两个组成部分。在 此外，核心还将开发新技术，以增强对骨骼和骨骼引起的变化的分析 teriparatide肌肉在各种动物研究中。核心的总体目标是继续： 答：提供高质量，高效和成本效益的基于组织的分子成像研究； B.提供高 在动物中的质量，一致，高效和成本效益的解剖学研究；和C.增强和 优化技术将新的尖端成像技术带入基础研究实践。具体 与目标C相关的施舍是：1。使用奥运会的VS100整体开发整个幻灯片成像技术 滑动成像仪以捕获组织学和其他数字化图像，以自动定量分析 组织形态的标准和新型参数； 2。开发定量方法来评估 使用免疫组织化学（IHC）和原位杂交（ISH）和VS100滑动成像仪的信号传导 软骨和断裂愈伤组织的组织样品； 3。继续发展鼠的组织微阵列（TMA） 组织以优化蛋白质表达的IHC分析，并使用整体自动量化表达 幻灯片图像仪； 4.优化用于硬件伪像抑制的算法并计算从 临床锥束CT（CB-CT）扫描以帮助评估同种异体移植物中同种异体移植物 大动物（犬）临床前模型。分子和解剖成像之间的这种翻译将是 由成像核心委员会促进，该委员会将监督两个组件的日常工作 核心并协助开发新技术来支持这些项目。