Molecular and Anatomic Imaging Core

分子和解剖成像核心

• 批准号: 7891429
• 负责人: Boyce Brendan
• 金额: $ 38.57万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7891429
• 关键词: Algorithms; Anatomy; Animals; Basic Science; Bone callus; Caring; Cartilage; Cell Culture Techniques; Clinical; Clinical Research; Communication; Confocal Microscopy; Development; Enhancement Technology; Ensure; Evaluation; Fracture; Fracture Healing; Future; Gene Expression; Human; Image; Imaging Techniques; Imaging technology; Joints; Knee joint; Lasers; Magnetic Resonance Imaging; Methodology; Molecular; Mus; Orthopedics; Pathology; Radiology Specialty; Resolution; Sampling; Services; Signal Pathway; Signal Transduction; Structure; Tissue Sample; Tissues; Translating; Translational Research; Translations; Trauma; articular cartilage; base; bone; clinical application; cone-beam computed tomography; cost; design; molecular imaging; new technology; novel strategies; software development

The Molecular and Anatomic Imaging Core will be used extensively by both the clinical an basic science components of all four of the projects. Utilization of the Core will be fairly equal among the four projects, although the services used will vary somewhat depending on the project focus. In combining molecular imaging and anatomic imaging under a single administrative structure, we have a unique opportunity not only to support translation in the sense of basic science to clinical application, but also to enable translation between molecular and anatomic imaging techniques, which can enhance both components. This will be facilitated by the structure we propose, which will consist of the Core co-directors in Pathology and Radiology who will oversee the molecular and anatomic services, respectively, as well as an internal committee which will help to ensure coordination, communication and translation between these services. The Core has 2 aims relating to provision of tissue-based molecular and clinical and animal anatomic imaging studies. In addition, there will be 3 subaims for technological development within the molecular component, and 3 subaims within the anatomic component, to develop new technologies and enhancements which could accelerate progress in the Project specific aims, and generate new approaches for future translational research. The aims are: 1. Provision of high quality, efficient, and cost-effective tissue-based molecular imaging studies. 2. Provision of high quality, consistent, efficient and cost-effective anatomic imaging studies in mice and humans. 3. Technology enhancement and refinement to bring new cutting-edge imaging technologies into basic and clinical research practice. Aim 3 has 6 subaims: Molecular Imaging Subaims: (A). Development of laser capture PCR for regional tissue gene expression analysis on samples of cartilage and bone related to the Projects. (B) Development of a quantitative methodology of evaluating signaling in cell culture using immunofluorescent confocal microscopy. (C) Quantitative signaling evaluation in tissue samples of cartilage or fracture callus confocal. Anatomic Imaging Subaims: (D) Development of software algorithms for clinical cone beam CT to quantify fracture healing in the presence of hardware. (E) Use of microCT to quantitate murine joint articular cartilage volumes. (F)Design of new micro-coils for high resolution MRI of mouse knee joints.

分子和解剖成像核将通过临床科学广泛使用 所有四个项目的组成部分。在四个项目中，核心的利用率将相同 尽管使用的服务会因项目重点而有所不同。在结合分子 成像和解剖成像在单个管理结构下，我们不仅有一个独特的机会 支持基础科学意义上的临床应用的翻译，但也可以启用翻译 在分子和解剖成像技术之间，可以增强这两个组件。这将是 我们提出的结构促进，该结构将由病理学的核心联合导演和 放射学分别监督分子和解剖服务的放射学以及内部 委员会将有助于确保这些服务之间的协调，沟通和翻译。 核心具有2个目标，该目标与提供基于组织的分子和临床和动物解剖学有关 成像研究。此外，分子内将有3个用于技术发展 组件中的组件和3个subiaims在解剖组件中开发新技术和增强 这可以加速项目的特定目标，并为未来生成新的方法 翻译研究。目的是：1。提供基于组织的高质量，高效和成本效益 分子成像研究。 2。提供高质量，一致，高效且具有成本效益的解剖学 小鼠和人类的成像研究。 3。提高技术和改进，带来新的尖端 成像技术成基础和临床研究实践。 AIM 3有6个subiaim： 分子成像subiaims：（a）。开发用于区域组织基因表达的激光捕获PCR 对与项目相关的软骨和骨骼样本的分析。 （b）开发定量 使用免疫荧光共聚焦显微镜评估信号传导的方法。 （C） 软骨或断裂愈伤组织共焦的组织样品中的定量信号传导评估。 解剖成像subiaims：（d）开发用于量化的临床锥束CT的软件算法 在存在硬件的情况下，断裂愈合。 （e）使用Microct定量鼠关节关节软骨 卷。 （f）设计新的微型机油，用于小鼠膝关节高分辨率MRI。