Epigenetic memory of plant stress response

植物逆境反应的表观遗传记忆

基本信息

批准号：
7676368
负责人：
Se Young Kim
金额：
$ 4.72万
依托单位：
RICE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-07-01 至 2011-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7676368
关键词：
Acclimatization Address Affect Animal Model Animals Antibodies Aplysia Arabidopsis Behavior Behavioral Biochemical Biological Assay Biological Models Biology Brain Calcium Chromatin Chromatin Structure Cognitive Complement Development ERG gene Environment Epigenetic Process Eukaryota Exposure to Food Gene Expression Gene Expression Regulation Gene Targeting Gene-Modified Health Histones Human Human Development Immediate-Early Genes Immunoblotting Investigation Learning Maintenance Mammals Mediating Memory Molecular Molecular Profiling Mouse-ear Cress Movement Neuronal Plasticity Neurons Organism Pathway interactions Phenotype Physiological Physiology Plants Proteins Reflex action Regulation Research Reverse Transcriptase Polymerase Chain Reaction Rodent Rodent Model Role Signal Transduction Simulate Stimulus Stream Stress System T-DNA Wood material base biological adaptation to stress candidate selection chromatin immunoprecipitation chromatin remodeling climate change cognitive function fitness histone modification improved insight mutant nervous system disorder neuropathology novel research study response

项目摘要

DESCRIPTION (provided by applicant): Epigenetic Memory of Plant Stress Response The ability to acquire information from a novel environment and present a defined change in behavior defines learning and memory. Although sessile, plants, nonetheless, respond to novel environmental conditions with alterations in gene expression and developmental phenotypes to improve fitness in a changing environment (Arnholdt-Schmitt, 2004). However, the mechanistic basis of plant memory of a stressful environment remains to be fully understood. Studies in Aplysia and in mammals have revealed a conservation of cellular mechanisms contributing to the biology of learning (Levenson and Sweatt, 2006). For example, the basis of memory formation lies with neuronal plasticity, wherein biochemical and structural changes, mediated through epigenetic control, facilitate adaptation. While many of the pathways utilized by animals during cognitive memory formation, such as calcium-dependent signaling and expression of early-response genes are similarly activated during plant stress reflex, the role of chromatin remodeling in plant stress response remains uninvestigated. As the main objective, this proposal strives to investigate the role of epigenetic regulation of chromatin structure, as a dynamic and sustainable paradigm utilized by plants to encode environmental stress, with two major aims using the model system Arabidopsis thaliana. Aim 1 will determine whether memory of abiotic stress exists in Arabidopsis, as evidenced through epigenetic regulation of gene expression. Aim 2 will investigate the epigenetic mechanisms in mediating learning and memory of abiotic stress in Arabidopsis. This will include the analysis of mutants in chromatin modifying genes and the identification of novel down-stream targets in plant epigenetic memory of stress response, using candidate selection and ChlP-CHIP screenapproaches. Health relevance: Investigation of epigenetic mechanisms of environmental stress memory in plants holds significant bearing in addressing the universal need for the maintenance of sustainable food crops despite a constantly changing global climate. Additionally, emerging discoveries of disruptions in molecular components of chromatin remodeling machinery resulting in human neurological disorders provide a testament to the crucial role of epigenetic mechanisms in development and human cognitive function. Sharing the fundamental eukaryotic attributes of epigenetic mechanisms, Arabidopsis represents an appealing model system, providing an alternative insight to how organismsform memory to store information about their surroundings

描述（由申请人提供）：植物应激反应的表观遗传记忆从新环境中获取信息并呈现明确的行为变化的能力定义了学习和记忆。尽管植物是无柄的，但它能够通过基因表达和发育表型的改变来响应新的环境条件，以提高在不断变化的环境中的适应性（Arnholdt-Schmitt，2004）。然而，植物对应激环境的记忆的机制基础仍有待充分了解。对海兔和哺乳动物的研究揭示了有助于学习生物学的细胞机制的保守性（Levenson 和 Sweatt，2006）。例如，记忆形成的基础在于神经元可塑性，其中通过表观遗传控制介导的生化和结构变化促进适应。虽然动物在认知记忆形成过程中利用的许多途径（例如钙依赖性信号传导和早期反应基因的表达）在植物应激反射过程中类似地被激活，但染色质重塑在植物应激反应中的作用仍未得到研究。作为主要目标，该提案致力于研究染色质结构的表观遗传调控的作用，作为植物用来编码环境胁迫的动态和可持续范例，使用拟南芥模型系统有两个主要目标。目标 1 将确定拟南芥中是否存在非生物胁迫记忆，这可以通过基因表达的表观遗传调控来证明。目标 2 将研究介导拟南芥非生物胁迫学习和记忆的表观遗传机制。这将包括使用候选选择和 ChlP-CHIP 筛选方法分析染色质修饰基因中的突变体以及识别植物应激反应表观遗传记忆中的新下游靶标。健康相关性：尽管全球气候不断变化，但对植物环境胁迫记忆的表观遗传机制的研究对于满足维持可持续粮食作物的普遍需求具有重要意义。此外，染色质重塑机制分子成分破坏导致人类神经系统疾病的新发现证明了表观遗传机制在发育和人类认知功能中的关键作用。拟南芥具有表观遗传机制的基本真核属性，代表了一个有吸引力的模型系统，为生物体如何形成记忆来存储周围环境信息提供了另一种见解