flow cytometry

流式细胞仪

• 批准号: 7339465
• 负责人: Charles L. Goolsby
• 金额: $ 14.76万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7339465
• 关键词: Accounting; Biological Assay; Cancer Center; Cell Line; Cell Separation; Cells; Clinical Sciences; Clinical Trials; Color; Complex; Comprehensive Cancer Center; Consultations; Core Facility; Cytometry; DNA analysis; Data Analyses; Data Quality; Disease; Equipment; Experimental Designs; Extrasensory Perception; Flow Cytometry; Focus Groups; Generations; Goals; Heterogeneity; Housing; Human Resources; Immunophenotyping; Individual; Institution; Laboratories; Lasers; Malignant Neoplasms; Measurement; Numbers; Operative Surgical Procedures; Preparation; Publishing; Quality Control; Research; Research Personnel; Sampling; Services; Sorting - Cell Movement; Speed; Training; Translational Research; Universities; assay development; base; cancer research center director; data acquisition; instrument; interest; medical schools; member; programs

The Robert H. Lurie Comprehensive Cancer Center Flow Cytometry Core Facility provides comprehensive flow cytometry and cell sorting services. The Flow Cytometry Core Facility serves investigators of the Cancer Center, Northwestern University Medical School, Northwestern University and other affiliated institutions by providing access to routine flow cytometry assays such as immunophenotyping and DNA analysis. However, research users encompass individuals with a broad spectrum of training in the basic and clinical sciences, and thus, a major goal of the facility is to provide the guidance, technical assistance, and equipment for investigators to utilize more complex multi-parametric, multi-laser measurements as well as cell sorting in their research regardless of their level of cytometry expertise. The equipment and expertise housed within the Core Facility permits implementation of most flow cytometry assays that have been published. Services provided by the facility personnel extend from consultation on experimental design, sample preparation and data analysis to instrument operation and set-up for cell-sorting and multi-laser operation. Because of the emphasis the facility places on the quality of data, the flow laboratory also offers services for sample preparation, data acquisition, and data analysis. In addition, the Core Facility has an active program of assay development guided by the interests of the investigators. As such, the Flow Cytometry Core Facility serves as a focus for individuals interested in cellular based measurements and cellular heterogeneity in disease providing critical support for cancer related research within the institution. This is also reflected by the Core Director's, and Cancer Center's, key founding involvement in the Cytometry Consortium, a multiinstitutional collaborative group focusing on complex multi-parametric cell based cytometry assay development in support of clinical trials and translational research. The facility includes two cell sorters; a five laser, 13 color MoFlo high speed cell sorter (Dako) and a two laser, six color Elite ESP (Beckman Coulter) cell sorter. In addition the facility has two benchtop flow cytometry analyzers; a 3 laser, 9 color Cyan (Dako) and a single laser, four color XL (Beckman Coulter). In addition, the flow lab has a ViCell cell .: counter/viability measurement instrument (Beckman Coulter) for cell line characterization and quality control for high speed sorting. In support of these instruments, the facility also has an offline analysis workstation providing the components for post acquisition data analysis and graphics generation. The Core Facility is now heavily utilized by a wide number of investigators (88), approximately 64.7% of which are Cancer Center members who account for approximately 73.0% of core utilization.

Robert H. Lurie 综合癌症中心流式细胞术核心设施提供全面的 流式细胞术和细胞分选服务。流式细胞术核心设施为癌症研究人员提供服务 中心、西北大学医学院、西北大学等附属机构 提供常规流式细胞术检测，例如免疫表型分析和 DNA 分析。然而， 研究用户包括在基础科学和临床科学领域接受过广泛培训的个人， 因此，该设施的一个主要目标是提供指导、技术援助和设备 研究人员利用更复杂的多参数、多激光测量以及细胞分选 无论他们的细胞计数专业知识水平如何，他们的研究都是如此。内部的设备和专业知识 核心设施允许实施大多数已发表的流式细胞术测定。服务 由设施人员提供的咨询延伸至实验设计、样品制备和 数据分析到仪器操作以及细胞分选和多激光操作的设置。因为 该设施非常重视数据质量，流量实验室还提供样品服务 准备、数据采集和数据分析。此外，核心设施还有一个积极的计划 检测开发以研究者的兴趣为指导。因此，流式细胞术核心设施 成为对基于细胞的测量和细胞异质性感兴趣的个人的焦点 疾病为机构内癌症相关研究提供关键支持。这也体现在 核心主任和癌症中心在细胞计数联盟（一个多机构组织）的创始过程中发挥了重要作用 专注于基于复杂多参数细胞的细胞计数分析的协作小组 支持临床试验和转化研究的发展。该设施包括两台细胞分选机；一个五 激光、13 色 MoFlo 高速细胞分选仪 (Dako) 和两激光、六色 Elite ESP (Beckman Coulter) 细胞分选仪。此外，该设施还有两台台式流式细胞术分析仪； 3 激光、9 色青色 (Dako) 和单激光、四色 XL (Beckman Coulter)。此外，流动实验室还有一个 ViCell 池： 用于细胞系表征和质量控制的计数器/活力测量仪器（Beckman Coulter） 用于高速分拣。为了支持这些仪器，该设施还设有离线分析工作站 提供用于采集后数据分析和图形生成的组件。核心设施是 现在被大量研究人员广泛使用 (88)，其中大约 64.7% 是癌症 中心成员约占核心利用率的73.0%。