MoFlo High Speed Multilaser Flow Cytometer/Cell Sorter

MoFlo 高速多激光流式细胞仪/细胞分选仪

• 批准号: 6581673
• 负责人: Charles L. Goolsby
• 金额: $ 44.08万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6581673
• 关键词: biomedical equipment purchase; cell sorting; flow cytometry; technology /technique development

DESCRIPTION (provided by applicant): Flow cytometry has become a key and critical research tool with applications in a wide range of disciplines in basic research, clinical translational research, and clinical studies. A wealth of reagents to identify specific cell sub-population, to study molecules regulating cell proliferation, apoptosis, signal transduction pathways, and functional states in cells are now available. Further, the fluorescent dyes and labeling techniques are now available to permit the simultaneous detection of many of these molecules and markers in individual cells. This is leading to the need for increasingly complex cytometric assays to understand the role and interaction of these molecules at the cell level. The Flow Cytometry Core Facility of the Robert H. Lurie Comprehensive Cancer Center at Northwestern University serves over 59 investigators with national peer reviewed funding of $22,631,071. This facility has a strong history of close, active interaction with its user base facilitating implementation of more difficult flow cytometry assays by all investigators regardless of their level of expertise. This coupled with the increase in available reagents has fostered increasingly complex requests of the facility. At present, the facility has a bench top analyzer, a Beckman-Coulter XLMLC four color instrument and a Beckman-Coulter ELITE ESP cell sorter which is over six years old. The current cell sorter has distinct limitations in the areas of complex, multi-laser and high speed sorting applications. Key for the Flow Cytometry Core Facility to meet the current and future needs of our user base is development of more complex and sophisticated multi-parameter and high-speed sorting capabilities. The requested MoFlo(TM) instrumentation will facilitate this critical development. This development will be aided by the MoFlo's enhanced multi-laser capabilities (three separate laser interrogation spots, focused/pinhole collection for all fluorescence detectors) and high speed sorting capabilities (25,000-50,000 cells/sec, 10-15X our current instrument capabilities). Further, the facilities oversight by Director, Dr. Charles Goolsby, with over 23 years experience in research and clinical flow cytometry and Mary Paniagua MT(ASCP)SI, with over 12 years experience in flow cytometry ensure that efficient implementation of these enhanced capabilities will occur.

描述（由申请人提供）：流式细胞术已成为一种重要且关键的研究工具，在基础研究、临床转化研究和临床研究等广泛学科中得到应用。现在有大量试剂可用于识别特定细胞亚群，研究调节细胞增殖、凋亡、信号转导途径和细胞功能状态的分子。此外，现在可以使用荧光染料和标记技术来同时检测单个细胞中的许多这些分子和标记。这导致需要越来越复杂的细胞分析来了解这些分子在细胞水平上的作用和相互作用。西北大学 Robert H. Lurie 综合癌症中心的流式细胞术核心设施为超过 59 名研究人员提供服务，国家同行评审资金为 22,631,071 美元。该设施有着与其用户群密切、积极互动的悠久历史，有助于所有研究人员实施更困难的流式细胞术测定，无论他们的专业水平如何。再加上可用试剂的增加，对设施的要求也日益复杂。目前，该设施拥有一台台式分析仪、一台贝克曼库尔特 XLMLC 四色仪和一台已有六年多的贝克曼库尔特 ELITE ESP 细胞分选仪。当前的细胞分选机在复杂、多激光和高速分选应用领域具有明显的局限性。流式细胞术核心设施满足用户群当前和未来需求的关键是开发更复杂和精密的多参数和高速分选功能。所需的 MoFlo(TM) 仪器将促进这一关键的开发。 MoFlo 增强的多激光能力（三个独立的激光询问点、所有荧光探测器的聚焦/针孔收集）和高速分选能力（25,000-50,000 个细胞/秒，是我们当前仪器能力的 10-15 倍）将有助于这一发展。此外，主任 Charles Goolsby 博士在研究和临床流式细胞术方面拥有超过 23 年的经验，Mary Paniagua MT(ASCP)SI 在流式细胞术方面拥有超过 12 年的经验，他们对设施的监督确保了这些增强功能的有效实施发生。