CONFOCAL MICROSCOPY CORE

共焦显微镜核心

• 批准号: 7529369
• 负责人: ANNA-LIISA NIEMINEN
• 金额: $ 7.87万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-06 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7529369
• 关键词: 3-Dimensional; Address; Advisory Committees; Arts; Cancer Center; Cells; Collaborations; Communities; Confocal Microscopy; Consultations; Core Facility; Educational process of instructing; Equipment; Fluorescence; Fostering; Hand; Housing; Image; Individual; Lasers; Life; Microscope; Microscopy; Operative Surgical Procedures; Optics; Phase; Photons; Physiologic pulse; Policies; Pulse taking; Reporting; Research Personnel; Reservations; Resolution; Scanning; Students; Supervision; Time; Tissues; Training; Universities; Viola; Visible Radiation; Week; day; experience; fluorescence microscope; fluorophore; instructor; instrument; instrumentation; member; new technology; optical imaging; programs; submicron; technology development

The objective of the Confocal Core Facility is to provide state-of-the-art instrumentation for examination of single living cells and tissues with submicron 3-dimensional resolution. The facility houses a Zeiss LSM 510 laser scanning confocal microscope equipped with multiple lasers to provide a variety of excitation lines. A tunable pulsed ThSapphire laser (700-900 nm) provides 200 femtosecond pulses for two and three-photon excitation of a variety of fluorophores whose one-photon excitation cross sections are in the ultra violet and visible light spectrum. An inverted Nikon Eclipse TE2000-S fluorescence microscope controlled by MetaMorph allows acquisition of wide-field fluorescence, bright field, and phase contrast images. The facility serves members of the Cancer Center, as well as the broader Case Western Reserve University community, and is open 7 days per week. Time on the instrument is by reservation only, and Cancer Center members will always have priority over nonmembers. The facility director has overall responsibility for the operations of the facility and provides expertise and consultation to core users in live cell confocal and multiphoton imaging. The facility manager has day-to-day responsibility for the microscopy component of the facility under the supervision of the director. An advisory committee meets annually to address any issues with facility operations, new technology development, and setting of policy. The advisory committee reports to the Senior Leaders Committee of the Cancer Center. The core is engaged in graduate teaching by organizing a 2-credit graduate course, Optical Microscopy and Imaging for Biologists. Students gain extensive hands-on training with state-of-the-art equipment for optical imaging guided by experienced academic instructors. Collectively, the Confocal Microscopy Core will have a large and positive impact on the Cancer Center's scientific programs. In addition, the use of the facility among different departments and centers will foster productive interactions between individual investigators to promote new collaborations.

共聚焦核心设施的目标是提供最先进的仪器来检查 具有亚微米 3 维分辨率的单个活细胞和组织。该设施配备一台 Zeiss LSM 510 激光扫描共焦显微镜配备多个激光器，提供多种激发线。一个 可调谐脉冲 ThSapphire 激光器 (700-900 nm) 为二光子和三光子提供 200 飞秒脉冲 激发各种荧光团，其单光子激发截面在紫外和 可见光谱。倒置 Nikon Eclipse TE2000-S 荧光显微镜由 MetaMorph 可以采集宽视场荧光、明视场和相差图像。设施 为癌症中心的成员以及更广泛的凯斯西储大学社区提供服务， 每周 7 天开放。仪器使用时间仅限预约，癌症中心会员 始终优先于非会员。设施总监全面负责运营 设施的负责人，并为活细胞共聚焦和多光子领域的核心用户提供专业知识和咨询 成像。设施经理对设施的显微镜组件负责日常工作 在导演的监督下。咨询委员会每年召开一次会议，以解决任何问题 设施运营、新技术开发和政策制定。咨询委员会向 癌症中心高级领导委员会。核心从事研究生教学工作的是 为生物学家组织 2 学分的研究生课程“光学显微镜和成像”。学生收获 由经验丰富的人员指导，使用最先进的光学成像设备进行广泛的实践培训 学术导师。总的来说，共焦显微镜核心将对 癌症中心的科学计划。此外，不同部门之间对该设施的使用以及 中心将促进各个研究人员之间富有成效的互动，以促进新的合作。