STRUCTURE OF A RAD4-RAD23 COMPLEX BOUND TO DNA

与 DNA 结合的 RAD4-RAD23 复合物的结构

• 批准号: 7721240
• 负责人: NIKOLA P PAVLETICH
• 金额: $ 0.97万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-15 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721240
• 关键词: Address; Binding; Complex; Computer Retrieval of Information on Scientific Projects Database; DNA; DNA lesion; Funding; Genome; Grant; Hand; Homologous Gene; Human; Institution; Lesion; Pathway interactions; Research; Research Personnel; Resources; Rest; Source; Structure; United States National Institutes of Health; Xeroderma Pigmentosum; Yeasts; adduct; base; protein structure; repaired

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The RAD4 (yeast homolog of human Xeroderma Pigmentosum C)-RAD23 complex acts early on in the global genome NER pathway, in parallel to DDB1-DDB2, to recognize DNA lesions such as 6-4 photoproduct and bulky base adducts. How a variety of chemically diverse lesions is recognized and handed to the rest of NER repair machinery are the major questions we are attempting to address by solving crystal structure of the protein complex bound to its cognate DNA.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 RAD4（人类色素干皮病 C 的酵母同源物）-RAD23 复合物在整体基因组 NER 通路的早期发挥作用，与 DDB1-DDB2 并行，识别 DNA 损伤，例如 6-4 光产物和大碱基加合物。 如何识别各种化学上不同的损伤并将其交给 NER 修复机制的其余部分是我们试图通过解决与其同源 DNA 结合的蛋白质复合物的晶体结构来解决的主要问题。