SWI2/SNF2 CHROMATIN-REMODELING DOMAIN OF EUKARYOTIC RAD54

真核 RAD54 的 SWI2/SNF2 染色质重塑结构域

• 批准号: 7721215
• 负责人: NIKOLA P PAVLETICH
• 金额: $ 0.97万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-15 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721215
• 关键词: ATP Hydrolysis; Amino Acid Motifs; Characteristics; Cleaved cell; Computer Retrieval of Information on Scientific Projects Database; DNA; DNA Binding; Deposition; Enzymes; Funding; Generations; Grant; Institution; Lobe; Mediating; Molecular Biology; Nature; Nucleosomes; Proteins; Publishing; Research; Research Personnel; Resources; SWI2/SNF2; Source; Structure; Torsion; United States National Institutes of Health; Work; Zebrafish; chromatin remodeling; helicase; homologous recombination

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. SWI2/SNF2 chromatin-remodeling proteins mediate the mobilization of nucleosomes and other DNA-associated proteins. SWI2/SNF2 proteins contain sequence motifs characteristic of SF2 helicases but do not have helicase activity. Instead, they couple ATP hydrolysis with the generation of superhelical torsion in DNA. The structure of the nucleosome-remodeling domain of zebrafish Rad54, a protein involved in Rad51-mediated homologous recombination, reveals that the core of the SWI2/SNF2 enzymes consist of two alpha/beta-lobes similar to SF2 helicases. The Rad54 helicase lobes contain insertions that form two helical domains, one within each lobe. These insertions contain SWI2/SNF2-specific sequence motifs likely to be central to SWI2/SNF2 function. A broad cleft formed by the two lobes and flanked by the helical insertions contains residues conserved in SWI2/SNF2 proteins and motifs implicated in DNA-binding by SF2 helicases. The Rad54 structure suggests that SWI2/SNF2 proteins use a mechanism analogous to helicases to translocate on dsDNA. This work was published in Nature Structural Molecular Biology and the structure deposited in the PDB (1Z3I).

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 SWI2/SNF2 染色质重塑蛋白介导核小体和其他 DNA 相关蛋白的动员。 SWI2/SNF2 蛋白含有 SF2 解旋酶特征的序列基序，但不具有解旋酶活性。相反，他们将 ATP 水解与 DNA 中超螺旋扭转的产生结合起来。斑马鱼 Rad54（一种参与 Rad51 介导的同源重组的蛋白质）的核小体重塑结构域的结构揭示了 SWI2/SNF2 酶的核心由两个类似于 SF2 解旋酶的 α/β 叶组成。 Rad54 解旋酶叶包含形成两个螺旋结构域的插入，每个叶内都有一个螺旋结构域。这些插入包含 SWI2/SNF2 特异性序列基序，可能是 SWI2/SNF2 功能的核心。由两个叶形成的宽裂口以及两侧的螺旋插入物包含 SWI2/SNF2 蛋白中保守的残基和与 SF2 解旋酶的 DNA 结合有关的基序。 Rad54 结构表明 SWI2/SNF2 蛋白使用类似于解旋酶的机制在 dsDNA 上易位。 该工作发表在《自然结构分子生物学》上，结构存入PDB（1Z3I）。