KINETIC STUDIES OF ENZYME MECHANISMS

酶机制的动力学研究

• 批准号: 7721623
• 负责人: WILLIAM Wallace CLELAND
• 金额: --
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721623
• 关键词: Carbon Dioxide; Computer Retrieval of Information on Scientific Projects Database; Diazomethane; Enzymes; Funding; Grant; Institution; Kinetics; Purpose; Research; Research Personnel; Resources; Source; United States National Institutes of Health; carbonic acid, monoanhydride with phosphoric acid, ion(2-); enzyme mechanism

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this project is to seek experimental evidence to support the hypothesis that some enzymes use carboxyphosphate as a CO2 source. Carboxyphosphate will be made synthetically and derivatized with diazomethane. The product will be characterized by 31P and 13C NMR.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 该项目的目的是寻找实验证据来支持某些酶使用羧磷酸盐作为二氧化碳来源的假设。 羧磷酸盐将通过合成方法制备并用重氮甲烷衍生化。 该产物将通过 31P 和 13C NMR 进行表征。