Portable Lab-on-a-chip Flow Cytometer: Prototype and Application Development

便携式芯片实验室流式细胞仪：原型和应用开发

基本信息

批准号：
8252284
负责人：
Yu-Hwa Lo
金额：
$ 49.86万
依托单位：
NANOCELLECT BIOMEDICAL, INC.
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-09-01 至 2014-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8252284
关键词：
Achievement Adopted Algorithms Architecture Biomedical Research Calculi Cell Line Cell Separation Cell surface Cells Clinical Code Color Consultations Databases Detection Development Devices Diagnostic Digital Signal Processing Discrimination Electronics Engineering Evaluation Event Eye Flow Cytometry Fluorescence Funding Future Goals Human Improve Access Injection of therapeutic agent Institutes Left Legal patent Life Liquid substance Medicine Methods Metric Microfluidics Modeling Molds Noise Performance Phase Photoreceptors Population Price Process Production Property Research Research Institute Research Personnel Running Side Signal Transduction Sorting - Cell Movement Speed System Techniques Technology Testing Time Travel Trees Tube United States National Institutes of Health Weight Work anticancer research base cell behavior cell type clinical application color detection commercialization computerized data processing cost design detector improved innovation instrument manufacturing process meetings micro-total analysis system operation outcome forecast particle photomultiplier point of care programs prototype research study shear stress success wasting

项目摘要

DESCRIPTION (provided by applicant): Portable Lab-on-a-chip Flow Cytometer: Prototype and Application Development NanoSort RESEARCH & RELATED Other Project Information 7. PROJECT SUMMARY The lab-on-a-chip NanoSort device proposed here advances the achievements of the Phase I Beta Prototype project. This device will be appropriate for commercial success by improving access to and reducing the cost and complexity of sorting flow cytometry. Furthermore, it will expand the applications that this device can serve, including translational and clinical point-of-care flow cytometry applications for diagnostics, prognosis, and personalized medicine. Having completed key milestones in the Phase I program, such as mas- producible injection molded chips, advanced signal detection and processing and key performance metrics, we propose to extend the capabilities to compete with best-in-class devices. Our Aims for this project are to create a device (NanoSort-1 or NS-1) that will have improved performance parameters of 6-color detection with a single photomultiplier tube (PMT), 4-way sorting using low shear stress piezoelectric (PZT) actuators, and a robust digital signal processing algorithm (Color-Space-Time (CoST) implementation that allows 10,000 events per second detection and sorting. In addition to these innovations, we will construct the device using current good manufacturing processes (cGMP) to assure that this device will be UL and CE certified and scalable to 10,000 units per year. We will validate this device with consultation from established researchers at UCSD and device manufactures (Becton-Dickinson and Life Technologies). PUBLIC HEALTH RELEVANCE: Portable Lab-on-a-chip Flow Cytometer: Prototype and Application Development NanoSort RESEARCH & RELATED Other Project Information 8. PROJECT NARRATIVE Important techniques in biomedical research include the counting (flow cytometry) and separation (cell sorting) of cells or particles based on their innate properties, currently performed by very large (~800lbs) and expensive (~$500,000) machines that require dedicated space and staff for their operation. We have invented and patented three techniques that enable the development of a sorting flow cytometer at a fraction of the cost and size of conventional instruments, using microfluidics to channel cells, piezoelectric actuators to sort cells, and a technique called Color-Space-Time coding to detect several colors with a single detector (as opposed to one detector per color in conventional instruments). Reductions in costs and complexity make this new nanosorting device accessible to any researcher, advancing biomedical discovery.

描述（由申请人提供）：便携式实验室芯片流式细胞仪：原型和应用程序开发Nanosort Research及相关其他项目信息7。通过改善对流式细胞术的成本和复杂性，该设备将适合商业成功。此外，它将扩大该设备可以使用的应用，包括用于诊断，预后和个性化医学的流式细胞流式细胞术应用。在I阶段计划中完成了关键里程碑，例如可生产的注入模制芯片，高级信号检测和处理以及关键性能指标，我们建议扩展功能以与一流的设备竞争。我们对该项目的目的是创建一种设备（Nanosort-1或NS-1），该设备将通过单个光层型管（PMT）（PMT）（使用低剪切应力压力电动（PZT）执行器（PZT）执行器，以及一个可靠的数字信号处理ALGORITHM（成本）实施，使用低剪切应力打电话（PZT），使用低剪切应力打电话（PZT）进行了4-路分类，以提高了6色检测的性能参数（PMT）。创新，我们将使用当前良好的制造工艺（CGMP）构建该设备，以确保该设备将获得UL和CE认证，并可扩展到每年10,000个单位，我们将通过UCSD和设备制造公司（Becton-Dickinson and Life Technologies and Device-Device and Techneries and Devisioners of Andosities consection condection and consities and consition and consition and consition and consition and consition and copsion and consities consity of Alection and cop of。公共卫生相关性：便携式实验室流式细胞仪：原型和应用开发纳米园研究及相关其他项目信息8。生物医学研究的项目叙事重要技术包括计数（流式细胞仪）和基于其天生的单元格和颗粒的分离（细胞分类），目前是由他们的天生而执行的，并要求他们的大型（〜800lbs）（〜800lbs）（〜800lbs）（〜800lbs）（〜800LBS）手术。我们已经发明并获得了专利的三种技术，这些技术可以以一小部分的成本和规模来开发传统仪器的成本和大小的一小部分，使用微流体来通道细胞，对电池进行分类细胞，以及一种称为Color Space时间编码的技术，以一种与单个检测器相反的颜色（根据一种探测器的颜色，可以在公会中检测几种颜色）。成本和复杂性的降低使任何研究人员都可以使用这种新的纳米分层设备，从而推进生物医学发现。