Research Support Core D: Molecular Pathology Core

研究支持核心 D：分子病理学核心

• 批准号: 8375138
• 负责人: MARY L HIXON
• 金额: $ 13.14万
• 依托单位: BROWN UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8375138
• 关键词: Address; Algorithms; Apoptotic; Basic Science; Biomedical Engineering; Bromodeoxyuridine; Cells; Chemical Exposure; Chemicals; Collaborations; Communities; Complex; Computer software; Consultations; DNA Repair; Diagnosis; Educational Intervention; Electron Microscopy; Embryo; Equipment; Exposure to; Freezing; Frozen Sections; Goals; Hand; Health; Hematoxylin and Eosin Staining Method; Histopathology; Image Analysis; Imagery; Immune; In Situ Nick-End Labeling; Individual; Laboratories; Lectin; Light; Methodology; Methods; Microscope; Microscopic; Microtomy; Modeling; Morphology; Paraffin; Paraffin Embedding; Pathology; Periodic acid Schiff stain method; Plastics; Procedures; Process; Research; Research Personnel; Research Support; Resources; Rhode Island; Sampling; Scanning; Specimen; Staining method; Stains; Structure; Students; Superfund; System; Technical Expertise; Techniques; Technology; Tissues; Toxicant exposure; Training; Transmission Electron Microscopy; Universities; base; cellular engineering; design; image processing; insight; instrumentation; investigator training; light microscopy; medical specialties; member; molecular pathology; nano; nanomaterials; novel; programs; remediation; soft tissue; virtual

The Molecular Pathology Core provides both biomedical and engineering researchers within the SBRP at Brown University the technical expertise and scientific equipment necessary to evaluate and diagnose pathological alterations from the nano to the organismal level following exposure paradigms ranging from the simple chemical exposure to the more complex chemical mixture. Core D enables investigators to apply histopathological, immunohistochemical and immunocytological methods in order to visualize morphology through various microscopic techniques. The strengths of Core D include specialized techniques for whole embryo embedding including serial and/or step sectioning. Core D provides thin sectioning capability for electron and transmission microscopy for the visualization of interactions between nanomaterials (engineering) and cells/tissue (biomedical). Routine procedures include the processing and embedding of specimens for paraffin, plastic and frozen sectioning for light microscopy. Routine staining procedures such as Periodic Schiff's Staining and Hematoxylin and Eosin stains. The methodology for specialty staining procedures such as immunostaining for apoptotic cells (TUNEL), damaged induced foci (yH2AX), unscheduled DNA synthesis (bromodeoxyuridine/Ki67), and staining for immune cells (lectin and GR1) following toxicant exposure are available. Core D also has a newly acquired vibratome for sectioning soft tissue an alternative for freezing and/or paraffin embedding of tissue. Another unique aspect of Core D is that it provides educational and hands on training for its instrumentation. The ScanScope CS system from Aperio Technologies is a virtual microscope which enables various SBRP laboratories to collect detailed scanning for image analysis of tissue specimens. The advanced algorithm software allows researchers to process images in order to identify and quantify morphological structures, thus reducing variability between individuals grading histopathology sections. In summary, Core D will continue to provide these resources through the following aims: 1. To provide expertise and consultation in the assessment of histological samples and assistance to Superfund project members and members of the Brown University community in the use of techniques in molecular pathology. 2. To provide training for investigators, students and technical staff in various technologies and interpretation of tissue morphology. 3. To anticipate the need for emerging pathology methodologies and establish expertise in their use. 4. To facilitate collaboration between SBRP investigators and the research community at large.

分子病理学核心提供了生物医学和工程研究人员 布朗大学SBRP评估和 暴露范式后，诊断从纳米到生物水平的病理改变 从简单的化学暴露到更复杂的化学混合物。核心D启用 研究人员采用组织病理学，免疫组织化学和免疫细胞学方法，以便 通过各种微观技术可视化形态。核心D的优势包括专业 整个胚胎嵌入的技术，包括串行和/或步骤切片。核心D提供薄 电子和传输显微镜的分段能力可视化相互作用 纳米材料（工程）和细胞/组织（生物医学）。常规程序包括处理和 用于光学显微镜的石蜡，塑料和冷冻切片的样品的嵌入。常规染色 诸如周期性席夫染色，苏木精和曙红染色之类的程序。方法 专业染色程序，例如凋亡细胞免疫染色（TUNEL），受损的诱导灶 （YH2AX），外生的DNA合成（溴脱氧尿苷/Ki67）和免疫细胞（凝集素和 gr1）可在有毒暴露量下进行。核心D还具有新获得的纤维状组件用于切片 软组织是组织冷冻和/或石蜡嵌入组织的替代方法。核心D的另一个独特方面是 它为其仪器提供了教育和动手培训。来自 Aperio Technologies是一种虚拟显微镜，使各种SBRP实验室能够收集详细的 扫描以进行组织样品的图像分析。高级算法软件允许研究人员 处理图像以识别和量化形态结构，从而降低 个体对组织病理学的分级分级。总而言之，核心D将继续提供这些资源 通过以下目的：1。在组织学评估中提供专业知识和咨询 对超级基金项目成员和布朗大学社区成员的样本和协助 在分子病理学中使用技术。 2。为调查人员，学生和技术提供培训 各种技术的人员和组织形态的解释。 3。预计需要出现 病理学方法并在其使用方面建立专业知识。 4。促进SBRP之间的合作 调查人员和整个研究社区。