CELL TO CELL COMMUNICATION IN MYCOBACTERIA

分枝杆菌中的细胞间通讯

• 批准号: 7725201
• 负责人: ANGELA MCKINNEY-WILLIAMS
• 金额: $ 2.42万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7725201
• 关键词: B-Lymphocytes; Body cavities; Cell Communication; Cells; Computer Retrieval of Information on Scientific Projects Database; DNA; Dominant-Negative Mutation; Epstein-Barr virus LMP-1 protein; Funding; Genus Mycobacterium; Grant; Herpesviridae; Human; Human Herpesvirus 4; Human Herpesvirus 8; Immunoprecipitation; In Vitro; Institution; Malignant Neoplasms; Mammalian Cell; Membrane Proteins; Play; Process; Proteins; Purpose; Research; Research Personnel; Resources; Role; Signal Transduction; Signaling Molecule; Simplexvirus; Source; Testing; Transfection; United States National Institutes of Health; Work; body cavity; cell transformation; in vivo; research study; tumor; tumorigenesis; vector

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In Dr. Zhang's lab I have continued researching the relationship between the Epstein Barr Virus and cancer. Epstein-Barr virus (EBV), a human herpesvirus, plays a critical role in oncogenesis of mammalian cells. The transformation process is primarily caused by the EBV latent membrane protein 1 (LMP-1). In vitro, EBV cannot transform primary B-cells without the expression of LMP-1. A dominant negative mutant of LMP-1 referred to as LMP-DM has been generated. The LMP-DM protein has the ability to inhibit cell signaling molecules which are normally regulated by LMP-1, specifically those involved in cancer formation . The purpose of this research was to test if LMP-DM has the ability to block the transformation function of LMP-1 in EBV transformed cells. A second project (May 2004-Dec2004) that I have worked on is looking at the relationship between the Human Herpesvirus 8 (HHV-8), Epstein Barr Virus (EBV), and body cavity tumor formation. Co-transfection experiments using vectors containing DNA from HHV-8 and EBV along with immunoprecipitation experiments were done to determine if HHV-8 and EBV cause tumor formation, specifically by causing the expression of tumor specific proteins in vivo.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 在张博士的实验室里，我继续研究EB病毒与癌症之间的关系。 Epstein-Barr 病毒 (EBV) 是一种人类疱疹病毒，在哺乳动物细胞的肿瘤发生中发挥着关键作用。 该转化过程主要由 EBV 潜伏膜蛋白 1 (LMP-1) 引起。在体外，如果没有 LMP-1 的表达，EBV 就无法转化原代 B 细胞。 LMP-1 的显性失活突变体（称为 LMP-DM）已产生。 LMP-DM 蛋白能够抑制通常由 LMP-1 调节的细胞信号分子，特别是那些参与癌症形成的信号分子。 本研究的目的是测试LMP-DM是否具有阻断EBV转化细胞中LMP-1的转化功能的能力。我从事的第二个项目（2004 年 5 月至 2004 年 12 月）是研究人类疱疹病毒 8 (HHV-8)、EB 病毒 (EBV) 和体腔肿瘤形成之间的关系。 使用含有 HHV-8 和 EBV DNA 的载体进行共转染实验以及免疫沉淀实验，以确定 HHV-8 和 EBV 是否导致肿瘤形成，特别是通过引起体内肿瘤特异性蛋白的表达。