REGULATION OF OVARIAN SURFACE EPITHELIAL CELLS

卵巢表面上皮细胞的调节

• 批准号: 7561867
• 负责人: Jay W Wright
• 金额: $ 3.79万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7561867
• 关键词: Cells; Cellular biology; Cessation of life; Computer Retrieval of Information on Scientific Projects Database; Cultured Cells; Data; Detection; Domestic Animals; Epithelial Cells; Epithelium; Event; Family suidae; Funding; Gene Expression; Genes; Goals; Grant; Human; In Vitro; Institution; Laboratories; Life; Macaca; Macaca mulatta; Malignant neoplasm of ovary; Menstrual cycle; Methods; Modeling; Molecular; Mus; Ovarian; Ovarian Mass; Ovary; Ovulation; Phase; Physiological; Prevention; Rattus; Regulation; Research; Research Personnel; Resources; Risk; Sheep; Source; Surface; Sus scrofa; Techniques; Time; Translating; United States National Institutes of Health; Woman; clinical application; comparative; in vivo; monolayer; nonhuman primate

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The ovarian surface epithelium (OSE) is a monolayer surrounding the ovary that comprises less than 1/1,000th of the ovarian mass, yet gives rise to nearly 90% of ovarian cancers in women. With a life-time risk of ovarian cancer almost 1 in 50, OSE cells must be considered among the most highly prone to transformation. This project was developed to consider a nonhuman primate model to investigate rhesus macaque OSE (RhOSE) cell biology in vitro and in vivo. Initial studies established RhOSE cell cultures and performed comparative analysis with human OSE cell cultures. These data showed high similarity between human and rhesus cells, with common gene expression not shared with OSE cells derived from laboratory or domestic animals, such as mouse, rat, sheep, or pig. The long-term goal of this project is to use cell culture, gene microarray, and other molecular techniques to determine how RhOSE proliferation and survival are regulated in vitro. Additional studies have begun to examine gene expression in the OSE in vivo, by collecting macaque ovaries from defined phases of the menstrual cycle. These studies will examine the normal fate of the OSE before and after ovulation and the regulation of OSE proliferation and death in the context of ovarian function. The results of these efforts may indicate which physiological events promote OSE transformation and could translate into clinical applications to develop more effective methods for prevention, detection, and treatment of ovarian cancer.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 卵巢表面上皮 (OSE) 是卵巢周围的单层，占卵巢质量的不到 1/1,000，但却是女性近 90% 卵巢癌的原因。 由于一生中罹患卵巢癌的风险接近五十分之一，OSE 细胞必须被视为最容​​易发生转化的细胞之一。 该项目的开发是为了考虑使用非人类灵长类动物模型来研究恒河猴 OSE (RhOSE) 细胞的体外和体内生物学。 初步研究建立了 RhOSE 细胞培养物，并与人类 OSE 细胞培养物进行了比较分析。 这些数据显示人类和恒河猴细胞之间具有高度相似性，与来自实验室或家畜（例如小鼠、大鼠、绵羊或猪）的 OSE 细胞不具有共同的基因表达。 该项目的长期目标是利用细胞培养、基因微阵列和其他分子技术来确定如何在体外调节 RhOSE 增殖和存活。 其他研究已经开始通过收集月经周期特定阶段的猕猴卵巢来检查体内 OSE 的基因表达。 这些研究将检查排卵前后 OSE 的正常命运以及卵巢功能背景下 OSE 增殖和死亡的调节。 这些努力的结果可能表明哪些生理事件促进 OSE 转化，并可转化为临床应用，以开发更有效的预防、检测和治疗卵巢癌的方法。