Mapping gene expression in the developing mouse brain

绘制小鼠大脑发育中的基因表达图谱

• 批准号: 8304264
• 负责人: JIANGYANG ZHANG
• 金额: $ 35.16万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2015-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8304264
• 关键词: Anatomy; Area; Atlases; Base of the Brain; Brain; Brain region; Cell Lineage; Cells; Computational Technique; Computer software; Data; Databases; Development; Disease; Embryo; Ensure; Exhibits; Foundations; Gene Expression; Gene Expression Profile; Genes; Genetic; Genetic Code; Goals; Grant; Hour; Human; Image; Imagery; Imaging Techniques; In Situ Hybridization; Laboratory mice; Lead; Magnetic Resonance; Magnetic Resonance Imaging; Maps; Measurement; Morus; Mus; Neonatal; Neuraxis; Neuroanatomy; Pattern; Phenotype; Play; Population; Process; Public Domains; Reference Values; Research; Resolution; Resources; Role; Series; Specificity; Staging; Structure; System; Techniques; Tissue Differentiation; Tissues; base; cell motility; cell type; mind control; postnatal; public health relevance; tool

DESCRIPTION (provided by applicant): Gene expression in the brain is important for understanding the genetic control of brain development. Because the laboratory mouse shares many genes and disease loci with human, its gene expression patterns are important for research in this area. With modern high throughput techniques, large scale gene expression data in the mouse brain are becoming available, and several mouse gene expression databases are being established. To integrate and analyze gene expression data with neuroanatomy in the embryonic and neonatal mouse brains, it is necessary to create three dimensional reference atlases of the developing mouse brain and tools that can map gene expression data into the atlas and perform analysis and visualization. The proposed study will establish a series of three dimensional reference atlases of the developing mouse brain based on high spatial resolution and rich tissue contrast provided by modern magnetic resonance imaging techniques. The atlases will contains structural annotation/segmentation, and more importantly, information on morphological changes during development obtained using advanced computational techniques. The proposed study will also develop tools that mapping gene expression data to the reference atlases. A selected group of gene expressions will be mapped, validated, and studied. The mapped gene expression data will also help us to define more detailed structures in the developing mouse brain atlases. PUBLIC HEALTH RELEVANCE: In this project, three dimensional atlases of the developing mouse brain will be developed to serve as the reference for mapping high resolution gene expression data. The atlases will consist of high resolution, rich contrast, and group average data that cover the key embryonic to neonatal stages during brain development. Techniques and software for mapping gene expression data to the atlases will also be developed, validated and used to investigate the relationship between gene expression and anatomical phenotypes as well as further enrich the structural annotation in the atlases.

描述（由申请人提供）：大脑中的基因表达对于理解大脑发育的遗传控制很重要。由于实验室小鼠与人类共有许多基因和疾病基因座，因此其基因表达模式对于该领域的研究很重要。使用现代的高吞吐量技术，小鼠脑中的大型基因表达数据正在可用，并且正在建立几种小鼠基因表达数据库。为了将基因表达数据与胚胎和新生小鼠大脑中的神经解剖结合在一起，有必要创建发育中的小鼠脑的三维参考地图集和可以将基因表达数据映射到地图集中并执行分析和可视化的三维参考地图。 拟议的研究将基于高空间分辨率和现代磁共振成像技术提供的高空间分辨率和丰富的组织对比度，建立发育中小鼠大脑的三维参考图形的系列。这些地图集将包含结构注释/分割，更重要的是，使用先进的计算技术获得的发展过程中形态变化的信息。拟议的研究还将开发将基因表达数据映射到参考图像库的工具。一组选定的基因表达式将进行映射，验证和研究。映射的基因表达数据还将帮助我们定义发育中的小鼠脑图中更详细的结构。 公共卫生相关性：在这个项目中，将开发出发育中的小鼠大脑的三维图书馆，以作为绘制高分辨率基因表达数据的参考。这些地图集将包括高分辨率，丰富的对比度和群体平均数据，这些数据涵盖了大脑发育过程中新生儿阶段的关键胚胎。还将开发，验证和用于研究基因表达与解剖表型之间的关系，并进一步丰富Atlases中的结构注释。