Mapping gene expression in the developing mouse brain

绘制小鼠大脑发育中的基因表达图谱

• 批准号: 8304264
• 负责人: JIANGYANG ZHANG
• 金额: $ 35.16万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2015-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8304264
• 关键词: Anatomy; Area; Atlases; Base of the Brain; Brain; Brain region; Cell Lineage; Cells; Computational Technique; Computer software; Data; Databases; Development; Disease; Embryo; Ensure; Exhibits; Foundations; Gene Expression; Gene Expression Profile; Genes; Genetic; Genetic Code; Goals; Grant; Hour; Human; Image; Imagery; Imaging Techniques; In Situ Hybridization; Laboratory mice; Lead; Magnetic Resonance; Magnetic Resonance Imaging; Maps; Measurement; Morus; Mus; Neonatal; Neuraxis; Neuroanatomy; Pattern; Phenotype; Play; Population; Process; Public Domains; Reference Values; Research; Resolution; Resources; Role; Series; Specificity; Staging; Structure; System; Techniques; Tissue Differentiation; Tissues; base; cell motility; cell type; mind control; postnatal; public health relevance; tool

DESCRIPTION (provided by applicant): Gene expression in the brain is important for understanding the genetic control of brain development. Because the laboratory mouse shares many genes and disease loci with human, its gene expression patterns are important for research in this area. With modern high throughput techniques, large scale gene expression data in the mouse brain are becoming available, and several mouse gene expression databases are being established. To integrate and analyze gene expression data with neuroanatomy in the embryonic and neonatal mouse brains, it is necessary to create three dimensional reference atlases of the developing mouse brain and tools that can map gene expression data into the atlas and perform analysis and visualization. The proposed study will establish a series of three dimensional reference atlases of the developing mouse brain based on high spatial resolution and rich tissue contrast provided by modern magnetic resonance imaging techniques. The atlases will contains structural annotation/segmentation, and more importantly, information on morphological changes during development obtained using advanced computational techniques. The proposed study will also develop tools that mapping gene expression data to the reference atlases. A selected group of gene expressions will be mapped, validated, and studied. The mapped gene expression data will also help us to define more detailed structures in the developing mouse brain atlases. PUBLIC HEALTH RELEVANCE: In this project, three dimensional atlases of the developing mouse brain will be developed to serve as the reference for mapping high resolution gene expression data. The atlases will consist of high resolution, rich contrast, and group average data that cover the key embryonic to neonatal stages during brain development. Techniques and software for mapping gene expression data to the atlases will also be developed, validated and used to investigate the relationship between gene expression and anatomical phenotypes as well as further enrich the structural annotation in the atlases.

描述（由申请人提供）：大脑中的基因表达对于理解大脑发育的遗传控制非常重要。由于实验室小鼠与人类共享许多基因和疾病位点，其基因表达模式对于该领域的研究非常重要。利用现代高通量技术，小鼠大脑中的大规模基因表达数据正在变得可用，并且正在建立多个小鼠基因表达数据库。为了将胚胎和新生小鼠大脑中的基因表达数据与神经解剖学进行整合和分析，有必要创建发育中的小鼠大脑的三维参考图谱以及可以将基因表达数据映射到图谱中并进行分析和可视化的工具。 拟议的研究将基于现代磁共振成像技术提供的高空间分辨率和丰富的组织对比度，建立一系列发育中小鼠大脑的三维参考图谱。该图集将包含结构注释/分割，更重要的是，包含使用先进计算技术获得的开发过程中形态变化的信息。拟议的研究还将开发将基因表达数据映射到参考图谱的工具。将绘制、验证和研究一组选定的基因表达。绘制的基因表达数据还将帮助我们在发育中的小鼠大脑图谱中定义更详细的结构。 公共健康相关性：在该项目中，将开发发育中的小鼠大脑的三维图谱，作为绘制高分辨率基因表达数据的参考。该图集将包含高分辨率、丰富的对比度和群体平均数据，涵盖大脑发育过程中胚胎到新生儿的关键阶段。还将开发、验证将基因表达数据映射到图谱的技术和软件，并用于研究基因表达和解剖表型之间的关系，并进一步丰富图谱中的结构注释。