CUMC: IMAGING CORE

CUMC：成像核心

• 批准号: 8360029
• 负责人: RICHARD John HALLWORTH
• 金额: $ 2.52万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360029
• 关键词: Argon; Cells; Fluorescence; Funding; Grant; Image; Lasers; Life; Microscope; National Center for Research Resources; Nebraska; Oils; Photons; Principal Investigator; Research; Research Infrastructure; Resolution; Resources; Scanning; Source; Spectrum Analysis; System; United States National Institutes of Health; Water; Work; base; cost; fluorophore; functional genomics; multi-photon; research study; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This imaging facility is a new multiuser core that is closely related to the scientific focus in structural biology. The core is based upon the acquisition of LSM 510 METANLO confocal scanning system with three lasers (Argon 458/477/488/514 nm, Green HeNe 543 nm, Red HeNe 633 nm. The confocal is built on a Zeiss AxioPlan 2IE MOT motorized upright microscope, with the following objectives: Plan Neo 40x oil 1.3 numerical aperture (n.a.) with differential interference contrast (DIC); Plan 63x 1.4 n.a. DIC; Achroplan 40x 0.8 n.a. water DIC, for live cell work; C-Apochromat 40x 1.2 n.a. DIC ,for fluorescence correlation spectroscopy; Plan Apo 10x; Plan Neo 20x 0.75 n.a. DIC. Initial funding for the core was through an NSF EPSCoR grant. The Core can image with most fluorophores, in either single-photon or multi-photon modes, with resolution approaching the theoretical limit (222 nm) The META multi-channel system enables separation of fluorophores with closely-spaced emission peaks by linear un-mixing. The types of experiments available far surpass those of conventional high resolution confocal imaging.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 该成像设施是一种新的多源核心，与结构生物学的科学重点密切相关。 核心是基于与三个激光器的LSM 510 Metanlo共聚焦扫描系统的获取（Argon 458/477/488/514 NM，Green Hene 543 nm，Red Hene 633 nm。共聚焦构建在Zeiss Axioplan 2ie Motization Numoirial Inmorial Inmocive上，构建了下面的NAIN NAIN OBER 1. （N.A）的差异（DIC）; EPSCOR授予的核心可以在大多数荧光团中，以单光子或多光子模式形象，分辨率接近理论限制（222 nm），元多通道系统可以通过线性固定的类型的实验来使荧光团分离具有紧密间隔的发射峰。