Molecular Assays for epidemiology studies

流行病学研究的分子测定

• 批准号: 8429277
• 负责人: DAVID HEIMBROOK
• 金额: $ 334.25万
• 依托单位: LEIDOS BIOMEDICAL RESEARCH, INC.
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-26 至 2018-09-25
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8429277
• 关键词: Area; Biological; Biological Assay; Biological Process; Blood; Blood specimen; Cell Line; Cells; Cervical; Clinical Research; Cryopreservation; Cytology; DNA; Detection; Diagnostic; Division of Cancer Epidemiology and Genetics; Electronics; Epidemiologic Studies; Epidemiology; Erythrocytes; Eye diseases; Feces; Fibroblasts; Flow Cytometry; Fluorescence-Activated Cell Sorting; Freeze Drying; Freezing; Hodgkin Disease; Human; Human Herpesvirus 4; Immunologic Monitoring; Immunologic Tests; In Vitro; Kaposi Sarcoma; Laboratories; Liquid substance; Malignant Neoplasms; Malignant Vaginal Neoplasm; Malignant neoplasm of brain; Malignant neoplasm of cervix uteri; Malignant neoplasm of esophagus; Malignant neoplasm of larynx; Malignant neoplasm of liver; Malignant neoplasm of lung; Malignant neoplasm of ovary; Malignant neoplasm of pancreas; Malignant neoplasm of pharynx; Malignant neoplasm of prostate; Malignant neoplasm of thyroid; Malignant neoplasm of urinary bladder; Mass Spectrum Analysis; Measurement; Molecular; Molecular Epidemiology; Multiple Myeloma; Mutation Detection; Nitrogen; Non-Hodgkin' s Lymphoma; Nucleic Acids; Paraffin Embedding; Pathogen detection; Pathogenesis; Peripheral Blood Mononuclear Cell; Phenotype; Plasma; Polymorphism Analysis; Preparation; Prevention; Process; Quality Control; RNA; Rectal Cancer; Renal carcinoma; Reporting; Research Infrastructure; Research Personnel; Scientist; Serum; Services; Single Nucleotide Polymorphism; Skin Cancer; Specimen; Specimen Handling; Spectrophotometry; Structure; Techniques; Technology; Tissues; Urine; Urologic Diseases; Uterine Cancer; Virus; Whole Blood; anticancer research; biobank; bioprocess; bone; cell immortalization; cold temperature; epidemiology study; instrumentation; leukemia; malignant breast neoplasm; malignant mouth neoplasm; malignant stomach neoplasm; melanoma; programs; quality assurance; repository; small molecule; tumor

Activities performed within this project provide DNA extraction, alliquotting, bioprocessing of human epidemiology specimcens for cancer research. Several facilities are utilized to process human specimens and to provide the necessary infrastructure to support human specimen processing and storage in support of epidemiology cancer research. The Mass Spectrometry Center is equipped with the latest in mass spectrometry (MS) instrumentation for analyzing both small and large biomolecules. The Center offers corresponding expertise in detection, quantitation, structure confirmation, and mass measurements. The Center also educates scientists on the use, interpretation, and applications of MS technologies. The CLIA-certified Molecular Diagnostics Laboratory provides state-of-the-art clinical and research diagnostics, including mutation detection, single nucleotide polymorphism analysis, and pathogen detection. The Human Immunological Monitoring Laboratory supports cervical cancer research by examining human host immunological parameters potentially important in the pathogenesis or prevention of virus-related tumors. The laboratory's capabilities include: receipt and processing of human specimens, including whole blood and cervical specimens; cryopreservation, DNA extraction and Epstein-Barr virus transformation of peripheral blood mononuclear cells (PBMC); immunological testing, including a variety of in vitro PBMC functional assays; HLA and PBMC phenotyping by flow cytometry (fluorescence activated cell sorting ¿ FACS); temporary low temperature storage of biological specimens and transfer to the DCEG biorepository; electronic tracking of biospecimens and reporting of assay results to DCEG investigators; maintain quality control/quality assurance programs; apply state-of-the-art techniques, as they become available. The Bioprocessing Laboratory processes biological specimens prior to their storage in the DCEG repository or dispersal to collaborators. The processing laboratory provides: processing and freezing of biological specimens, including whole blood, serum, plasma, urine, and feces; lyophilization of specimens; flash-freezing of tissue in liquid nitrogen; separation and viable cryopreservation of cell lines, including the routine assessment of viability; cryopreservation of red blood cells to maintain enzymatic activity; establishment of cell lines; large scale propagation of cell lines; nucleic acid extraction; distribution of specimens. The DNA Extraction Laboratory extracts DNA from whole blood specimens, blood fractions, transformed lymphoblastoid lines, cultured fibroblasts, tumor specimens, buccal cells, paraffin-embedded tissue, Guthrie cards, cytology preparations, or other biological specimens. Activities performed within this project provide DNA extraction, alliquotting, bioprocessing of human epidemiology specimens for cancer research. Human molecular epidemiology research is focused in the following areas: Bone Bladder cancer Brain cancer Breast cancer Cervical cancer Colo-rectal cancer Esophageal cancer Eye disease Kaposi's sarcoma Kidney cancer Hodgkin's disease Laryngeal cancer Leukemia Liver cancer Lung cancer Melanoma Multiple Myeloma Non-Hodgkin Lymphoma Pancreatic cancer Pharyngeal cancer Prostate cancer Oral cancer Ovarian cancer Skin cancer Stomach cancer Testicluar cancer Thyroid cancer Uterine cancer Urologic disorders Vaginal cancer

该项目开展的活动为癌症研究提供人类流行病学样本的 DNA 提取、等分和生物处理，并利用多个设施来处理人类样本，并提供必要的基础设施来支持人类样本处理和存储，以支持流行病学癌症研究。光谱测量中心配备了最新的质谱 (MS) 仪器，用于分析小和大生物分子。该中心提供检测、定量、结构确认和质量测量方面的相应专业知识。还对科学家进行 MS 技术的使用、解释和应用进行培训。经 CLIA 认证的分子诊断实验室提供最先进的临床和研究诊断，包括突变检测、单核苷酸多态性分析和人类病原体检测。免疫监测实验室通过检查在病毒相关肿瘤的发病机制或预防中可能重要的人类宿主免疫参数来支持宫颈癌研究。该实验室的能力包括：接收和处理人类标本，包括全血和宫颈标本；外周血单核细胞 (PBMC) 的冷冻保存、DNA 提取和 Epstein-Barr 病毒转化，包括通过流式细胞术进行的各种体外 PBMC 功能测定（荧光激活细胞分选 ¿ FACS）；生物样本的临时低温储存并转移至 DCEG 生物样本库；向 DCEG 研究人员报告检测结果；应用最先进的技术；生物处理实验室在将生物样本存储在 DCEG 存储库或分发给合作者之前对其进行处理。处理实验室提供：生物样本的处理和冷冻，包括全血、血清、血浆、尿液和血液。粪便；液氮中的组织快速冷冻；细胞系的分离和存活性冷冻保存；红细胞的冷冻保存以维持细胞系的大规模增殖；核酸提取；样本分配 DNA 提取实验室从全血样本、血液成分、转化的淋巴母细胞系、培养的成纤维细胞、肿瘤样本中提取 DNA。口腔细胞、石蜡包埋组织、Guthrie 卡、细胞学制剂或其他生物标本。该项目中进行的活动为癌症研究提供人类流行病学标本的 DNA 提取、等分和生物处理。骨癌 膀胱癌 脑癌 乳腺癌 宫颈癌 结直肠癌 食道癌 眼病 卡波西肉瘤 肾癌霍奇金病 喉癌 白血病 肝癌 肺癌 黑色素瘤 多发性骨髓瘤 非霍奇金淋巴瘤 胰腺癌 咽癌 前列腺癌 口腔癌 卵巢癌 皮肤癌 胃癌 睾丸癌 甲状腺癌 子宫癌 泌尿系统疾病 阴道癌