Molecular Assays for epidemiology studies

流行病学研究的分子测定

• 批准号: 8429277
• 负责人: DAVID HEIMBROOK
• 金额: $ 334.25万
• 依托单位: LEIDOS BIOMEDICAL RESEARCH, INC.
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-26 至 2018-09-25
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8429277
• 关键词: Area; Biological; Biological Assay; Biological Process; Blood; Blood specimen; Cell Line; Cells; Cervical; Clinical Research; Cryopreservation; Cytology; DNA; Detection; Diagnostic; Division of Cancer Epidemiology and Genetics; Electronics; Epidemiologic Studies; Epidemiology; Erythrocytes; Eye diseases; Feces; Fibroblasts; Flow Cytometry; Fluorescence-Activated Cell Sorting; Freeze Drying; Freezing; Hodgkin Disease; Human; Human Herpesvirus 4; Immunologic Monitoring; Immunologic Tests; In Vitro; Kaposi Sarcoma; Laboratories; Liquid substance; Malignant Neoplasms; Malignant Vaginal Neoplasm; Malignant neoplasm of brain; Malignant neoplasm of cervix uteri; Malignant neoplasm of esophagus; Malignant neoplasm of larynx; Malignant neoplasm of liver; Malignant neoplasm of lung; Malignant neoplasm of ovary; Malignant neoplasm of pancreas; Malignant neoplasm of pharynx; Malignant neoplasm of prostate; Malignant neoplasm of thyroid; Malignant neoplasm of urinary bladder; Mass Spectrum Analysis; Measurement; Molecular; Molecular Epidemiology; Multiple Myeloma; Mutation Detection; Nitrogen; Non-Hodgkin' s Lymphoma; Nucleic Acids; Paraffin Embedding; Pathogen detection; Pathogenesis; Peripheral Blood Mononuclear Cell; Phenotype; Plasma; Polymorphism Analysis; Preparation; Prevention; Process; Quality Control; RNA; Rectal Cancer; Renal carcinoma; Reporting; Research Infrastructure; Research Personnel; Scientist; Serum; Services; Single Nucleotide Polymorphism; Skin Cancer; Specimen; Specimen Handling; Spectrophotometry; Structure; Techniques; Technology; Tissues; Urine; Urologic Diseases; Uterine Cancer; Virus; Whole Blood; anticancer research; biobank; bioprocess; bone; cell immortalization; cold temperature; epidemiology study; instrumentation; leukemia; malignant breast neoplasm; malignant mouth neoplasm; malignant stomach neoplasm; melanoma; programs; quality assurance; repository; small molecule; tumor

Activities performed within this project provide DNA extraction, alliquotting, bioprocessing of human epidemiology specimcens for cancer research. Several facilities are utilized to process human specimens and to provide the necessary infrastructure to support human specimen processing and storage in support of epidemiology cancer research. The Mass Spectrometry Center is equipped with the latest in mass spectrometry (MS) instrumentation for analyzing both small and large biomolecules. The Center offers corresponding expertise in detection, quantitation, structure confirmation, and mass measurements. The Center also educates scientists on the use, interpretation, and applications of MS technologies. The CLIA-certified Molecular Diagnostics Laboratory provides state-of-the-art clinical and research diagnostics, including mutation detection, single nucleotide polymorphism analysis, and pathogen detection. The Human Immunological Monitoring Laboratory supports cervical cancer research by examining human host immunological parameters potentially important in the pathogenesis or prevention of virus-related tumors. The laboratory's capabilities include: receipt and processing of human specimens, including whole blood and cervical specimens; cryopreservation, DNA extraction and Epstein-Barr virus transformation of peripheral blood mononuclear cells (PBMC); immunological testing, including a variety of in vitro PBMC functional assays; HLA and PBMC phenotyping by flow cytometry (fluorescence activated cell sorting ¿ FACS); temporary low temperature storage of biological specimens and transfer to the DCEG biorepository; electronic tracking of biospecimens and reporting of assay results to DCEG investigators; maintain quality control/quality assurance programs; apply state-of-the-art techniques, as they become available. The Bioprocessing Laboratory processes biological specimens prior to their storage in the DCEG repository or dispersal to collaborators. The processing laboratory provides: processing and freezing of biological specimens, including whole blood, serum, plasma, urine, and feces; lyophilization of specimens; flash-freezing of tissue in liquid nitrogen; separation and viable cryopreservation of cell lines, including the routine assessment of viability; cryopreservation of red blood cells to maintain enzymatic activity; establishment of cell lines; large scale propagation of cell lines; nucleic acid extraction; distribution of specimens. The DNA Extraction Laboratory extracts DNA from whole blood specimens, blood fractions, transformed lymphoblastoid lines, cultured fibroblasts, tumor specimens, buccal cells, paraffin-embedded tissue, Guthrie cards, cytology preparations, or other biological specimens. Activities performed within this project provide DNA extraction, alliquotting, bioprocessing of human epidemiology specimens for cancer research. Human molecular epidemiology research is focused in the following areas: Bone Bladder cancer Brain cancer Breast cancer Cervical cancer Colo-rectal cancer Esophageal cancer Eye disease Kaposi's sarcoma Kidney cancer Hodgkin's disease Laryngeal cancer Leukemia Liver cancer Lung cancer Melanoma Multiple Myeloma Non-Hodgkin Lymphoma Pancreatic cancer Pharyngeal cancer Prostate cancer Oral cancer Ovarian cancer Skin cancer Stomach cancer Testicluar cancer Thyroid cancer Uterine cancer Urologic disorders Vaginal cancer

该项目中执行的活动提供了DNA提取，分析，对癌症研究的人类流行病学标本的生物处理。几种设施用于处理人类标本，并提供必要的基础设施，以支持人类标本的加工和存储以支持流行病学癌症研究。质谱中心配备了最新的质谱（MS）仪器，用于分析小生物分子和大型生物分子。中心提供了相应的检测，定量，结构确认和质量测量方面的专业知识。该中心还对科学家进行了MS技术的使用，解释和应用。经CLIA认证的分子诊断实验室提供了最先进的临床和研究诊断，包括突变检测，单核苷酸多态性分析和病原体检测。人类免疫监测实验室通过检查人类宿主免疫学参数可能在病毒相关肿瘤的发病机理或预防中可能重要的人类免疫学参数来支持宫颈癌研究。实验室的功能包括：人类标本的收据和加工，包括全血和宫颈标本；周围血液单核细胞的冷冻保存，DNA提取和爱泼斯坦 - 巴尔病毒转化（PBMC）；免疫学测试，包括各种体外PBMC功能测定；通过流式细胞仪（荧光激活的细胞排序„ FACS）HLA和PBMC表型；生物测量的暂时低温存储并转移到DCEG生物座位上；对生物测量的电子跟踪和对DCEG研究者的测定结果报告；维护质量控制/质量保证计划；应用最新技术，因为它们可用。生物处理实验室工艺在存储DCEG存储库之前或分散到合作者之前将其生物标本。加工实验室提供：生物标本的加工和冻结，包括全血，血清，血浆，尿液和粪便；标本的冻干；液氮液体中组织的闪光冻结；细胞系的分离和生存的冷冻保存，包括常规的生存能力评估；红细胞的冷冻保存以维持酶活性；建立细胞系；细胞系的大规模传播；核酸提取；标本的分布。 DNA提取实验室从全血标本，血液部分，转化的淋巴生物细胞系，培养的成纤维细胞，肿瘤标本，颊细胞，石蜡包裹的组织，Guthrie Cards，细胞学制剂制剂或其他生物学标本中提取DNA。该项目中执行的活动提供了DNA提取，分析，对癌症研究的人类流行病学标本的生物处理。人分子流行病学研究集中在以下领域：骨膀胱癌脑癌乳腺癌乳腺癌宫颈癌共结肠癌直肠癌食管癌眼科癌症Kaposi的肉瘤肾脏癌Hodgkin病Laryngeal Laryngeal白细胞白血病癌症胃癌睾丸癌甲状腺癌子宫癌泌尿科疾病阴道癌