Using Arabidopsis to uncover interactions between phytohormone signaling pathways

利用拟南芥揭示植物激素信号通路之间的相互作用

• 批准号: 8306724
• 负责人: Lucia Strader
• 金额: $ 24.57万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-15 至 2014-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8306724
• 关键词: Abscisic Acid; Affinity Chromatography; Agriculture; Arabidopsis; Area; Auxins; Binding; Biochemical; Biochemical Genetics; Blood Vessels; Cell division; Characteristics; Complement; Development; Dissection; Embryonic Development; Environment; Ethylenes; Excision; Exhibits; Fruit; Genes; Genetic Research; Genetic Transcription; Germination; Goals; Growth; Growth and Development function; Hormones; Knowledge; Learning; Lesion; MAPK Signaling Pathway Pathway; Mentors; Mitogen-Activated Protein Kinases; Molecular; Molecular Genetics; Mouse-ear Cress; Mutation; Nature; Pathway interactions; Perception; Phenotype; Phosphoric Monoester Hydrolases; Plant Growth Regulators; Plant Leaves; Plant Model; Plant Roots; Plants; Postdoctoral Fellow; Principal Investigator; Protein Biosynthesis; Protein phosphatase; Proteins; Regulation; Reporter; Repressor Proteins; Research; Resistance; Resources; Rice; Role; Saline; Seeds; Signal Pathway; Signal Transduction; Site; Soil; Specificity; Stress; Students; Testing; Time; Training; Universities; base; biological adaptation to stress; career; indoleacetic acid; insight; mutant; novel; positional cloning; research study; response; senescence; stress tolerance; tool

PROJECT SUMMARY - INTERACTION OF PHYTOHORMONE SIGNALING PATHWAYS Candidate: The candidate's long-term career goal is to become a principal investigator in an academic setting, studying the interaction networks involved in phytohormone response. During the training period, she will learn biochemical research approaches to complement her graduate applied agricultural and post- doctoral molecular genetic research. Training Environment: Rice University provides an ideal training environment because of its quality research and intimate setting. Dr. Bonnie Bartel, the mentor for this project, is a leader in Arabidopsis research. Further, she is an active mentor who devotes the majority of her time to her research and her postdocs and students. Research: The long-term goal of this project is to enhance understanding of the signaling network that connects the phytohormones auxin, abscisic acid (ABA), and ethylene. The dual-specificity protein phosphatase, IBR5, the subject of this proposal, is a novel point of interaction between auxin, ABA, and ethylene signal transduction networks. Several interconnected approaches will use IBR5 to gain greater understanding of the relationship among these phytohormones. Firstly, IBR5-interacting proteins will be identified (Aim 1); these may include substrates and regulators of the IBR5 phosphatase. Additionally, second-site ibr5 modifiers will be analyzed (Aim 2) to determine whether sensitivity to auxin, ABA, and ethylene can be separated, or if they are interconnected in inseparable ways. Identification of defective genes in these modifiers (Aim 3) will allow molecular elucidation of interactions between these phytohormone pathways. These studies will contribute to understanding of which signaling components are shared among these three pathways and which are not shared, and will identify additional nodes in the phytohormone signaling network. In addition to expanding our basic knowledge, a detailed understanding of these three phytohormones, involved in growth responses, stress tolerance, and fruit ripening, may provide insight for the eventual improvement of plants of agricultural or medicinal importance. For instance, determining how to increase stress tolerance (ABA perception) without altering growth characteristics (auxin perception) or ripening (ethylene perception) could be key to adapting plants to high stress areas (i.e., areas of low rainfall or saline soils).

项目摘要 - 植物激素信号通路的相互作用 候选人：候选人的长期职业目标是成为学术领域的首席研究员 设置，研究涉及植物激素反应的相互作用网络。培训期间， 她将学习生物化学研究方法，以补充她的研究生应用农业和后 博士分子遗传学研究。 培训环境：莱斯大学以其优质的品质提供了理想的培训环境 研究和亲密的环境。该项目的导师 Bonnie Bartel 博士是拟南芥领域的领军人物 研究。此外，她是一位积极的导师，将大部分时间投入到她的研究和她的研究中。 博士后和学生。 研究：该项目的长期目标是增强对信号网络的理解 连接植物激素生长素、脱落酸 (ABA) 和乙烯。双特异性蛋白 磷酸酶 IBR5 是本提案的主题，是生长素、ABA 和 乙烯信号转导网络。几种相互关联的方法将使用 IBR5 来获得更大的收益 了解这些植物激素之间的关系。首先，IBR5 相互作用蛋白将 确定（目标 1）；这些可能包括 IBR5 磷酸酶的底物和调节剂。此外， 将分析第二位点 ibr5 修饰符（目标 2）以确定是否对生长素、ABA 和 乙烯可以分离，或者如果它们以不可分离的方式相互连接。缺陷识别 这些修饰物中的基因（目标 3）将允许分子阐明这些修饰物之间的相互作用 植物激素途径。这些研究将有助于了解哪些信号成分是 在这三个路径之间共享以及不共享的路径，并将识别路径中的其他节点 植物激素信号网络。除了拓展我们的基础知识之外，详细的了解 这三种植物激素与生长反应、应激耐受性和果实成熟有关，可能 为最终改良具有农业或药用价值的植物提供见解。例如， 确定如何在不改变生长特性（生长素 感知）或成熟（乙烯感知）可能是使植物适应高胁迫地区（即 降雨量少或盐碱地）。