Using Arabidopsis to uncover interactions between phytohormone signaling pathways

利用拟南芥揭示植物激素信号通路之间的相互作用

• 批准号: 7772531
• 负责人: Lucia Strader
• 金额: $ 8.71万
• 依托单位: RICE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-15 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7772531
• 关键词: Abscisic Acid; Affinity Chromatography; Agriculture; Arabidopsis; Area; Auxins; Binding; Biochemical; Biochemical Genetics; Blood Vessels; Cell division; Characteristics; Complement; Development; Dissection; Embryonic Development; Environment; Ethylenes; Excision; Exhibits; Fruit; Genes; Genetic Research; Genetic Transcription; Germination; Goals; Growth; Growth and Development function; Hormones; Knowledge; Learning; Lesion; MAPK Signaling Pathway Pathway; Mentors; Mitogen-Activated Protein Kinases; Molecular; Molecular Genetics; Mouse-ear Cress; Mutation; Nature; Pathway interactions; Perception; Phenotype; Phosphoric Monoester Hydrolases; Plant Growth Regulators; Plant Leaves; Plant Model; Plant Roots; Plants; Postdoctoral Fellow; Principal Investigator; Protein Biosynthesis; Protein phosphatase; Proteins; Regulation; Reporter; Repressor Proteins; Research; Research Training; Resistance; Resources; Rice; Role; Saline; Seeds; Signal Pathway; Signal Transduction; Site; Soil; Specificity; Stress; Students; Testing; Time; Training; Universities; base; biological adaptation to stress; career; indoleacetic acid; insight; mutant; novel; positional cloning; public health relevance; research study; response; senescence; stress tolerance; tool

DESCRIPTION (provided by applicant): Candidate: The candidate's long-term career goal is to become a principal investigator in an academic setting, studying the interaction networks involved in phytohormone response. During the training period, she will learn biochemical research approaches to complement her graduate applied agricultural and post- doctoral molecular genetic research. Training Environment: Rice University provides an ideal training environment because of its quality research and intimate setting. Dr. Bonnie Bartel, the mentor for this project, is a leader in Arabidopsis research. Further, she is an active mentor who devotes the majority of her time to her research and her postdocs and students. Research: The long-term goal of this project is to enhance understanding of the signaling network that connects the phytohormones auxin, abscisic acid (ABA), and ethylene. The dual-specificity protein phosphatase, IBR5, the subject of this proposal, is a novel point of interaction between auxin, ABA, and ethylene signal transduction networks. Several interconnected approaches will use IBR5 to gain greater understanding of the relationship among these phytohormones. Firstly, IBR5-interacting proteins will be identified (Aim 1); these may include substrates and regulators of the IBR5 phosphatase. Additionally, second-site ibr5 modifiers will be analyzed (Aim 2) to determine whether sensitivity to auxin, ABA, and ethylene can be separated, or if they are interconnected in inseparable ways. Identification of defective genes in these modifiers (Aim 3) will allow molecular elucidation of interactions between these phytohormone pathways. These studies will contribute to understanding of which signaling components are shared among these three pathways and which are not shared, and will identify additional nodes in the phytohormone signaling network. In addition to expanding our basic knowledge, a detailed understanding of these three phytohormones, involved in growth responses, stress tolerance, and fruit ripening, may provide insight for the eventual improvement of plants of agricultural or medicinal importance. For instance, determining how to increase stress tolerance (ABA perception) without altering growth characteristics (auxin perception) or ripening (ethylene perception) could be key to adapting plants to high stress areas (i.e., areas of low rainfall or saline soils). Public Health Relevance: Auxin, abscisic acid, and ethylene are three plant hormones controlling many aspects of growth and development, such as growth responses, stress tolerance, and fruit ripening. Understanding of the signaling interactions between theses phytohormones, in addition to contributing to the general knowledge of how these hormones act, may provide insight for the eventual improvement of agriculturally- and medicinally-important plants. For instance, determining how to increase stress tolerance (ABA perception) without altering growth characteristics (auxin perception) or ripening (ethylene perception) could be key to adapting crops to high stress areas (i.e., areas of low rainfall or saline soils).

描述（由申请人提供）： 候选人：候选人的长期职业目标是成为学术环境中的首席研究员，研究植物激素反应中涉及的相互作用网络。在培训期间，她将学习生物化学研究方法，以补充她的研究生应用农业和博士后分子遗传学研究。 培训环境：莱斯大学因其高质量的研究和温馨的环境而提供了理想的培训环境。该项目的导师 Bonnie Bartel 博士是拟南芥研究领域的领军人物。此外，她还是一位积极的导师，将大部分时间投入到她的研究以及博士后和学生上。 研究：该项目的长期目标是增强对连接植物激素生长素、脱落酸 (ABA) 和乙烯的信号网络的了解。双特异性蛋白磷酸酶 IBR5 是本提案的主题，是生长素、ABA 和乙烯信号转导网络之间的一个新的相互作用点。几种相互关联的方法将使用 IBR5 来更好地了解这些植物激素之间的关系。首先，将鉴定 IBR5 相互作用蛋白（目标 1）；这些可能包括 IBR5 磷酸酶的底物和调节剂。此外，还将分析第二位点 ibr5 修饰符（目标 2），以确定对生长素、ABA 和乙烯的敏感性是否可以分开，或者它们是否以不可分割的方式相互关联。鉴定这些修饰因子中的缺陷基因（目标 3）将有助于从分子角度阐明这些植物激素途径之间的相互作用。这些研究将有助于了解这三种途径之间共享哪些信号成分，哪些不共享，并将确定植物激素信号网络中的其他节点。除了扩展我们的基础知识之外，对这三种涉及生长反应、胁迫耐受性和果实成熟的植物激素的详细了解，可以为最终改良具有农业或药用价值的植物提供见解。例如，确定如何在不改变生长特性（生长素感知）或成熟（乙烯感知）的情况下提高胁迫耐受性（ABA感知）可能是使植物适应高胁迫地区（即降雨量少或盐渍土地区）的关键。 公共健康相关性：生长素、脱落酸和乙烯是三种植物激素，控制生长和发育的许多方面，例如生长反应、胁迫耐受性和果实成熟。了解这些植物激素之间的信号相互作用，除了有助于了解这些激素如何发挥作用之外，还可以为农业和药用重要植物的最终改良提供见解。例如，确定如何在不改变生长特性（生长素感知）或成熟（乙烯感知）的情况下提高胁迫耐受性（ABA感知）可能是使作物适应高压力地区（即降雨量少或盐碱地地区）的关键。