GENE EXPRESSION

基因表达

• 批准号: 7414806
• 负责人: PETER D. EMANUEL
• 金额: $ 11.11万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7414806
• 关键词: Bioinformatics; Biometry; Cancer Center; Data; Data Analyses; Development; Diagnosis; Experimental Designs; Funding; Gene Expression; Genome; Human; Hybridization Array; Knowledge; Malignant Neoplasms; Microarray Analysis; Polymerase Chain Reaction; RNA purification; Range; Research Personnel; Services; Specialist; Spottings; Technology; Time; Work; data acquisition; design; interest; rapid technique; research study

DESCRIPTION (provided by applicant): Genome-scale gene expression analysis is rapidly changing how we study, diagnose and treat human cancers. In order to meet the scientific needs of Cancer Center investigators for gene expression analyses, the Gene- Expression Shared Facility was established in early 2004. The facility provides support to Cancer Center investigators interested in microarray technology. The facility was recently established using Cancer Center developmental funds and is focused primarily on the study of gene expression analyses as they relate to human malignancy. The facility includes both Affymetrix and spotted array technologies and has incorporated into it a strong bioinformatics network to support the facility. Real-time quantitative PCR analysis has been included in order to provide a rapid method to validate hits from microarray experiments. The Shared Facility has been designed specifically to: 1) provide a comprehensive facility for global-scale gene expression studies with a emphasis on human malignancies; 2) provide a broad range of services in order to meet the scientific needs of a variety of Cancer Center investigators including basic researchers and clinicians; 3) work closely with the Biostatistics and Bioinformatics Shared Facility to provide investigators with experimental design, data analysis and data interpretation support; 4) educate investigators and work to increase knowledge and understanding of microarray technology in order to most appropriately utilize the technologies. The facility provides full support including experimental design, RNA purification, probe synthesis, array hybridizations, data acquisition and storage, and full bioinformatics support. The facility is staffed by two Co-directors, two technical staff and a bioinformatics specialist.

描述（由申请人提供）：基因组规模的基因表达分析正在迅速改变我们研究、诊断和治疗人类癌症的方式。 为了满足癌症中心研究人员对基因表达分析的科学需求，基因表达共享设施于 2004 年初建立。该设施为对微阵列技术感兴趣的癌症中心研究人员提供支持。 该设施最近是利用癌症中心发展资金建立的，主要致力于与人类恶性肿瘤相关的基因表达分析研究。 该设施包括 Affymetrix 和斑点阵列技术，并融入了强大的生物信息学网络来支持该设施。 实时定量 PCR 分析是为了提供一种快速方法来验证微阵列实验的命中结果。 共享设施专门设计用于：1）为全球范围的基因表达研究提供综合设施，重点关注人类恶性肿瘤； 2) 提供广泛的服务，以满足包括基础研究人员和临床医生在内的各种癌症中心研究人员的科学需求； 3）与生物统计学和生物信息学共享设施密切合作 为研究者提供实验设计、数据分析和数据解释支持； 4） 教育研究人员并努力增加对微阵列技术的知识和理解，以便最适当地利用这些技术。 该设施提供全面支持，包括实验设计、RNA 纯化、探针合成、阵列杂交、数据采集和存储以及完整的生物信息学支持。 该设施由两名联合主任、两名技术人员和一名生物信息学专家组成。