CORE--TRANSGENIC MICE

核心——转基因小鼠

• 批准号: 7470546
• 负责人: Louis French Reichardt
• 金额: $ 19.49万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7470546
• 关键词: Animals; Core Facility; Cultured Cells; Equipment; Female; Genotype; Injection of therapeutic agent; Knock-out; Laboratories; Mus; Mutation; Persons; Postdoctoral Fellow; Principal Investigator; Program Research Project Grants; Specialist; Students; Supervision; Technology; Training; Transgenic Animals; Transgenic Mice; Transgenic Organisms; blastocyst; embryo cell; embryonic stem cell; experience; male; member; programs; size

The majority of projects in this program project grant will rely heavily on transgenic mice, some of which have already been generated and others of which need to be generated. During the past five years, existence of this core has greatly facilitated the ability of the laboratories directed by Rob Edwards, David Copenhagen, Louis Reichardt and Michael Stryker to exploit this powerful technology. During the next five years, we anticipate expanded use of this core that will almost certainly be used by all members of our new program project team. The existence of a core has made it possible to hire a senior specialist with more than 15 years of relevant experience in preparing transgenic mice plus a junior technician able to manage the mouse colonies and perform routine genotyping, etc. The former person has helped our laboratories generate more than a dozen transgenic lines and half a dozen knockout strains. The more junior technician has been particularly valuable in helping the labs entering this field. All laboratories will continue to benefit from a transgenic core with a person dedicated to performing well certain tasks of critical importance in generating such animals. To help culture and screen ES cells, provide transgenic animals with targeted mutations and provide help in training postdoctoral fellows and graduate students in this important technology, we propose to maintain a modestly-sized and staffed core facility with a cell culture facility for embryonic stem cells, equipment for injection of single cell embryos and blastocysts, and a mouse colony with fertile and vasectomized males and fertile females for use as single cell embryo or blastocyst donors and pseudopregnant recipients. The Reichardt laboratory will provide the supervision and expertise for setting up and managing this facility under the overall guidance of the Principal Investigator.

该计划项目拨款中的大部分项目将严重依赖转基因小鼠，其中一些已经产生，另一些需要产生。在过去的五年里，这个核心的存在极大地促进了罗布·爱德华兹（Rob Edwards）、大卫·哥本哈根（David Copenhagen）、路易斯·雷查特（Louis Reichardt）和迈克尔·史赛克（Michael Stryker）领导的实验室利用这项强大技术的能力。在接下来的五年中，我们预计该核心的使用将得到扩大，几乎肯定会被我们新项目团队的所有成员使用。有了核心的存在，就可以聘用一位具有15年以上转基因小鼠制备相关经验的高级专家，再加上一位能够管理小鼠群体、进行常规基因分型等的初级技术人员。前者为我们实验室提供了帮助产生了十多个转基因品系和六个基因敲除品系。更初级的技师 在帮助实验室进入这一领域特别有价值。所有实验室都将继续受益于转基因核心，并有专人致力于出色地完成某些对产生此类动物至关重要的任务。为了帮助培养和筛选胚胎干细胞，为转基因动物提供有针对性的突变，并为培训博士后研究员和研究生提供这项重要技术的帮助，我们建议维持一个规模适中、配备人员充足的核心设施，其中包括胚胎干细胞的细胞培养设施、注射单细胞胚胎和囊胚的设备，以及具有可育和输精管切除的雄性和可育雌性的小鼠群体，用作单细胞胚胎或囊胚供体和假孕受体。 Reichardt 实验室将在首席研究员的全面指导下为该设施的建立和管理提供监督和专业知识。