Molecular Resources Core

分子资源核心

• 批准号: 8059136
• 负责人: Randal A Skidgel
• 金额: $ 31.43万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2016-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8059136
• 关键词: Blood Vessels; Communication; Endothelial Cells; Generations; Genotype; Human Resources; Knockout Mice; Lung; Mass Spectrum Analysis; Measurement; Methods; Molecular; Molecular Biology; Oxidants; Permeability; Peroxonitrite; Principal Investigator; Proteomics; Quality Control; Reagent; Research; Research Personnel; Resources; Role; Services; Signal Transduction; Site; Small Interfering RNA; Specialist; Speed; Training; Transfection; base; design; expression vector; instrumentation; lung vascular injury; meetings; mutant; professor; programs

Summary: Core C, the Molecular Resources Core, will provide molecular reagents and services to all four projects and will be crucial to the completion of the specific aims of each proposal in the Program Project. The proposal for a Molecular Resources Core is a continuation of the original Core C. The overall function of Core C is to provide molecular reagents and expertise, including generation of deletion and site-directed mutants and incorporation into the desired expression vectors, generation of adenoviral and retroviral constructs for transducfion of primary endothelial cells, genotyping of knockout mice, designing and generating appropriate siRNA based constructs, sequencing and verification of constructs obtained from other investigators, molecular biology training to project personnel, and overseeing and maintaining instrumentation for efficient transfection of endothelial cells. Core C will also provide expertise for proteomic analysis and oxidant measurements. This core will be directed by Dr. Randal A. Skidgel and molecular reagents and services will be provided by a Senior Research Specialist (Dr. Tiffany Sharma), Research Specialist (Debra Salvi) and Research Assistant Professor (Dr. Fulong Tan). Dr. Neil Kelleher, Professor and Director of the Proteomics Center of Excellence, will advise, oversee and carry out proteomic analysis by mass spectrometry. Dr. Marcelo Bonini, Assistant Professor, will provide expertise, advice and will carry out measurement of peroxynitrite and other oxidants to dissect their roles in regulating lung vascular endothelial barrier barrier function. Consolidating these efforts in Core C will result in increased speed and efficiency in accomplishing the research tasks outlined in each of the projects and uniformity of approaches and methods so that results from each project can be compared. In addition, it will allow strict quality control and consistency of molecular reagents to be used by the various projects. Furthermore, an added and important benefit of the centralized Core C will be to markedly decrease expenses compared to a scenario in which each project would generate its own molecular resources. Regular meetings of the Core Leader with the Senior Research Specialist and project leaders will assure coordination and prioritization of the generation of molecular reagents and assure that the desired reagents are provided in a timely manner to the component projects.

概括： 核心 C，即分子资源核心，将为所有四个项目提供分子试剂和服务，对于完成计划项目中每个提案的具体目标至关重要。分子资源核心的提案是原始核心 C 的延续。核心 C 的总体功能是 提供分子试剂和专业知识，包括生成缺失和定点突变体并掺入所需的表达载体、生成用于转导原代内皮细胞的腺病毒和逆转录病毒构建体、敲除小鼠的基因分型、设计和生成适当的基于 siRNA 的构建体、测序和验证从其他研究人员获得的构建体，对项目人员进行分子生物学培训，以及监督和维护有效转染内皮细胞的仪器。 Core C 还将提供蛋白质组分析和氧化剂测量方面的专业知识。这 核心将由 Randal A. Skidgel 博士指导，分子试剂和服务将由高级研究专家 (Tiffany Sharma 博士)、研究专家 (Debra Salvi) 和研究助理教授 (Fulong Tan 博士) 提供。 Neil Kelleher 博士，蛋白质组学卓越中心教授兼主任， 将通过质谱法提供建议、监督和进行蛋白质组分析。助理教授 Marcelo Bonini 博士将提供专业知识和建议，并将对过氧亚硝酸盐和其他氧化剂进行测量，以剖析它们在调节肺血管内皮屏障功能中的作用。巩固这些努力 核心 C 将提高完成每个项目中概述的研究任务的速度和效率，以及方法和方法的统一性，以便可以比较每个项目的结果。此外，它将允许各个领域使用的分子试剂进行严格的质量控制和一致性。 项目。此外，与每个项目生成自己的分子资源的情况相比，集中式 Core C 的另一个重要好处是显着降低费用。核心领导者与高级研究专家和项目负责人的定期会议将确保协调 确定分子试剂生成的优先顺序，并确保及时向组成项目提供所需的试剂。