Circular Dichroism Spectrometer

圆二色性光谱仪

• 批准号: 7388703
• 负责人: DENIS L. ROUSSEAU
• 金额: $ 15.02万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7388703
• 关键词: 18 year old; Antineoplastic Agents; Area; Arts; Autoimmune Diseases; Blood Substitutes; Campylobacter jejuni; Circular Dichroism; Clinical Research; Clinical Trials; Clinical Trials Design; Communities; Condition; Development; Disease; Enzymes; Equilibrium; Fiber; Fluorescence Spectroscopy; Future; Goals; Host resistance; Human; Internet; Mails; Medicine; Mycobacterium tuberculosis; Neurofibrillary Tangles; Optics; Parkinson Disease; Pharmaceutical Preparations; Pharmacologic Substance; Process; Proteins; Public Health; Schedule; Site; T-Cell Leukemia; Temperature; Titrations; United States National Institutes of Health; absorption; antimicrobial; college; inhibitor/antagonist; instrument; instrumentation; member; pathogen; relating to nervous system; synuclein

DESCRIPTION (provided by applicant): The goal of this project is to purchase a state-of-the-art high sensitivity Circular Dichroism Spectrometer (JASCO J-815) and to make it available to the entire Albert Einstein College of Medicine scientific community. This instrument will replace an 18-year old CD spectrometer that has much lower sensitivity, poor reliability and limited functions. The capabilities of the new JASCO instrument will extend well beyond those of any instrument currently available on the campus, as it will combine circular dichroism, optical absorption and fluorescence spectroscopies that can be made under equilibrium, titration, temperature-controlled and stopped-flow conditions. There are several users, with National Institutes of Health support, who have an essential need of the instrumentation to fulfill the aims of their projects. Once on campus it is anticipated that many other members of the scientific community will take advantage of this instrumentation to carry out their projects as well. The availability of the instrument will be announced by e-mail and a full description of its capabilities, along with scheduling information and technical documents, will be permanently available on a new web site. The planned projects with the instrumentation span a wide spectrum of biomedical areas including mechanistic studies of several enzymatic processes in physiologically important enzymes, studies of proteins that are believed to be involved in conferring drug and/or host resistance in the human pathogens, M. tuberculosis and C. jejuni, characterization of blood substitutes, which are in clinical trials, design and characterization of transition state inhibitors, which are in clinical trials for leukemia T-cell autoimmune disease, and determination of the fiber formation processes in 1-synuclein, the principle component of the neural tangle aggregates in Parkinson's disease. This instrumentation will be very relevant to public health in both the near and long term. It will immediately yield better understanding and advances in the development of blood substitutes and anti-cancer drugs. In the longer term, it will give new information on critical proteins from human pathogens, which may allow for new anti-microbial strategies, and finally, new mechanistic information on several human enzymes will be valuable for future clinical studies including the understanding of diseases and the development of new pharmaceutical agents.

描述（由申请人提供）：该项目的目标是购买一台最先进的高灵敏度圆二色性光谱仪 (JASCO J-815) 并将其提供给整个阿尔伯特·爱因斯坦医学院科学界。该仪器将取代已有 18 年历史的 CD 光谱仪，后者灵敏度低得多，可靠性差且功能有限。新的 JASCO 仪器的功能将远远超出校园中现有的任何仪器，因为它将结合圆二色性、光学吸收和荧光光谱，可以在平衡、滴定、温度控制和停流条件下进行。在美国国立卫生研究院的支持下，有一些用户迫切需要仪器来实现其项目目标。一旦进入校园，预计科学界的许多其他成员也将利用该仪器来开展他们的项目。该仪器的上市将通过电子邮件公布，其功能的完整描述以及日程安排信息和技术文件将在新网站上永久提供。 计划使用该仪器的项目涵盖广泛的生物医学领域，包括对生理上重要的酶中的几种酶促过程的机制研究，对被认为与人类病原体、结核分枝杆菌中的药物和/或宿主耐药性有关的蛋白质的研究空肠弯曲菌，血液替代品的表征（正在进行临床试验），过渡态抑制剂的设计和表征（正在进行白血病 T 细胞自身免疫性疾病的临床试验），以及测定 1-突触核蛋白（帕金森病神经缠结聚集体的主要成分）中的纤维形成过程。 该仪器在短期和长期内都与公共卫生非常相关。它将立即带来对血液替代品和抗癌药物开发的更好理解和进步。从长远来看，它将提供有关人类病原体关键蛋白质的新信息，这可能会允许新的抗微生物策略，最​​后，有关几种人类酶的新机制信息将对未来的临床研究有价值，包括了解疾病和新药剂的开发。