Human and Mouse Cell Isolation and Phenotyping

人类和小鼠细胞分离和表型分析

• 批准号: 8391963
• 负责人: Andrew S Weyrich
• 金额: $ 53.22万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-01 至 2017-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8391963
• 关键词: Biological Assay; Blood; Blood Clot; Blood Platelets; Blood coagulation; Breeding; Cell Separation; Cells; Center for Translational Science Activities; Diabetes Mellitus; Ensure; Equipment; Generations; Glucose; Housing; Human; In Vitro; Incubators; Instruction; Lipids; Maintenance; Medicine; Megakaryocytes; Metabolic; Metabolic syndrome; Molecular Medicine; Mus; Non-Insulin-Dependent Diabetes Mellitus; Obesity; Patients; Phenotype; Platelet Function Tests; Population; Research Personnel; Research Project Grants; Resources; Risk; Services; Sterility; Thrombosis; Tissues; Universities; Utah; Work; comparative; diabetes management; in vivo; insight; mouse model; programs

PROJECT SUMMARY (See instructions): The Human and Mouse Cell Isolation and Phenotyping Core (Core C) will provide services for all four research projects in the University of Utah Molecular Medicine Translational Research Center in Thrombosis (U2M2-TRCT) program. Core C is centrally located and immediately available to all U2M2-TRCT investigators. The core will isolate human and mouse megakaryocytes and platelets for U2M2-TRCT investigators, perform standard in vitro and in vivo platelet function assays, assist with generation, breeding, and maintenance of genetically manipulated mice proposed in projects 1 and 2, and manage mouse models of obesity and diabetes. Core C is led by a group of investigators with strengths and expertise in all of the essential core activities. In order to standardize populations of megakaryocytes and platelets among studies, Core C will isolate and culture megakaryocytes and platelets for all U2M2-TRCT investigators. In this regard. Core C will communicate directly with Core B to ensure seamless isolation of platelets from patients with obesity and diabetes. The Core will also directly communicate with projects 1 and 2 for cell isolation, metabolic phenotyping, and related functional analyses. The Core is equipped with a cell sorter and sterile incubators for isolation and culture of purified human and mouse megakaryocytes and platelets. In addition, the core has equipment for in vitro and in vivo analyses of platelet function and metabolic phenotyping of mice that will be used in projects 1 and 2. Core investigators will also work together to generate, breed, and maintain colonies of mice that are proposed in projects 1 and 2. The U2M2-TRCT program will use the expertise and resources provided by Core C to guide and direct studies proposed in projects 1-4.

项目摘要（参见说明）： 人类和小鼠细胞分离和表型分析核心（核心 C）将为犹他大学血栓形成分子医学转化研究中心 (U2M2-TRCT) 项目的所有四个研究项目提供服务。 Core C 位于中心位置，所有 U2M2-TRCT 研究人员均可立即使用。该核心将分离人类和小鼠巨核细胞和血小板，用于 U2M2-TRCT 研究人员进行标准的体外和体内血小板功能测定，协助项目 1 和 2 中提出的基因操纵小鼠的生成、繁殖和维护，并管理肥胖和糖尿病小鼠模型。核心 C 由一组在所有重要核心活动方面具有优势和专业知识的研究人员领导。为了标准化研究中的巨核细胞和血小板群体，Core C 将为所有 U2M2-TRCT 研究人员分离和培养巨核细胞和血小板。对此。 Core C 将直接与 Core B 通信，以确保从肥胖和糖尿病患者中无缝分离血小板。核心还将直接与项目 1 和 2 进行沟通，以进行细胞分离、代谢表型分析和相关功能分析。 Core 配备了细胞分选仪和无菌 用于分离和培养纯化的人和小鼠巨核细胞和血小板的培养箱。此外，核心还拥有用于体外和体内分析小鼠血小板功能和代谢表型的设备，这些设备将用于项目 1 和 2。核心研究人员还将共同努力生成、繁殖和维持小鼠群体，这些小鼠项目 1 和 2 中提出。U2M2-TRCT 计划将使用 核心 C 提供的专业知识和资源来指导和指导项目 1-4 中提出的研究。