Optopathology by Light Microscopy and Molecular Techniques

光学显微镜和分子技术的光病理学

• 批准号: 8195316
• 负责人: MICHAEL J. MCKENNA
• 金额: $ 56.75万
• 依托单位: MASSACHUSETTS EYE AND EAR INFIRMARY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2016-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8195316
• 关键词: Acoustic Neuroma; Acoustics; Adipose tissue; Affect; Animal Model; Atrophic; Audiometry; Auditory; Auditory Brainstem Responses; Autopsy; Behavior; Belief; Bilateral; Biopsy; Birth; Brain; Cells; Cessation of life; Clinical; Clinical Management; Cochlea; Cochlear Nerve; Collaborations; Communities; DNA; Data; Diagnosis; Disease; Doctor of Philosophy; Ear Diseases; Endosteum; Equilibrium; Excision; Fiber; Functional disorder; Future; Genomics; Goals; Guidelines; Hearing; Heart; Histologic; Human; Hyalin; Individual; Invaded; Knowledge; Laboratories; Labyrinth; Lead; Lesion; Life; Ligaments; Liquid Chromatography; Medicine; Mentorship; Methods; Mitotic; Molecular; Molecular Medicine; Molecular Target; National Institute on Deafness and Other Communication Disorders; Nature; Nerve; Neurilemmoma; Neurofibromatosis 2; Nuclear; Operative Surgical Procedures; Organ; Otolaryngology; Otology; Otosclerosis; Pancreas; Pathogenesis; Pathologic; Pathology; Pharmacotherapy; Physiology; Prevention; Process; Proteomics; Pure-Tone Audiometry; Regenerative Medicine; Registries; Research; Research Personnel; Resources; Scala Tympani; Scanning; Science; Sense Organs; Sensorineural Hearing Loss; Sensory; Severities; Supporting Cell; System; Techniques; Temporal bone structure; Testing; Time; Training; Training Programs; X-Ray Computed Tomography; base; bone; career; cell determination; clinical Diagnosis; clinical decision-making; clinical practice; design; hearing impairment; improved; insight; light microscopy; member; neoplastic cell; planetary Atmosphere; round window; tool; tumor

DESCRIPTION (provided by applicant): Knowledge of the pathologic basis of disease is central to the study of medicine, including disorders affecting the auditory and vestibular systems. Otology is unique in that the inner ear is inaccessible during life and so, conventional techniques of pathologic study such as biopsy and surgical excision are not feasible. Consequently, insight into the pathologic basis of ear disease can only be obtained by postmortem study of temporal bones and by developing credible animal models. An improved understanding of the pathology and pathogenesis of disorders affecting the auditory and vestibular systems should lead to more rational diagnosis and clinical management of these diseases. The procurement, processing and study of human temporal bones is a time-consuming and costly endeavor which lies outside the purview of clinical departments of pathology, and is therefore a research endeavor that is performed in temporal bone laboratories. This proposal was developed in keeping with the guidelines announced in RFA-DC-11-003 that is meant to establish the NIDCD Otopathology Research Collaboration Network. Our goals include the following: 1. Investigate pathology of hearing loss caused by cochleovestibular schwannoma. 2. Investigate radiologic-histologic correlations in otosclerosis, and the pathogenetic mechanism of sensorineural hearing loss when otosclerosis involves the cochlea. 3. Investigate dynamics of sensory and non-sensory cell turnover in the human inner ear. 4. Establish a program for the training of future temporal bone researchers. 5. Cooperate with the NIDCD Otopathology Research Collaboration Network. The proposed research is designed to provide new knowledge and insight into the pathology and pathophysiology of otosclerosis, acoustic tumors and cell turnover in the inner ear, which, in turn, should lead to better methods of diagnosis and therapy. PUBLIC HEALTH RELEVANCE: We are proposing to use light microscopy and tools of molecular medicine to conduct research into the pathology and pathogenesis of common disorders of the ear (otosclerosis, acoustic tumors and cell turnover in the inner ear), which, in turn, should lead to better methods of diagnosis and therapy.

描述（由申请人提供）：疾病病理学基础的知识是医学研究的核心，包括影响听觉和前庭系统的疾病。耳鼻喉科是独一无二的，因为内耳在生活中是无法访问的，因此，诸如活检和手术切除等病理学研究的传统技术是不可行的。因此，只有通过对时间骨骼的尸体研究和开发可靠的动物模型才能获得对耳部疾病的病理基础的见解。对影响听觉和前庭系统的疾病的病理和发病机理的改进理解应导致对这些疾病的更合理诊断和临床管理。人类颞骨的采购，加工和研究是一项耗时且昂贵的努力，位于病理学临床部门的范围之内，因此是在颞骨实验室中进行的一项研究努力。该提案是根据旨在建立NIDCD耳病学研究合作网络的RFA-DC-11-003中宣布的指南制定的。我们的目标包括以下内容：1。研究由耳目骨鱼鞘瘤造成的听力损失的病理。 2。研究耳效硬化中的放射学 - 历史相关性，以及耳鼻喉病涉及耳蜗时感觉到性听力丧失的致病机制。 3。研究人内耳中感觉和非感官细胞转换的动力学。 4.建立一个培训未来颞骨研究人员的计划。 5。与NIDCD局部病理学研究合作网络合作。拟议的研究旨在提供有关耳鼻喉性，声学肿瘤和内耳细胞更新的病理学和病理生理学的新知识和洞察力，这反过来又将导致更好的诊断和治疗方法。 公共卫生相关性：我们建议使用光学显微镜和分子医学的工具来研究耳朵常见疾病的病理和发病机理（耳鼻喉科，声学肿瘤和内耳中细胞更新），反过来，这应该导致更好的诊断和治疗方法。