Long-term function of vocal fold replacement in swine

猪声带置换的长期功能

• 批准号: 10537089
• 负责人: Jennifer L Long
• 金额: --
• 依托单位: VA GREATER LOS ANGELES HEALTHCARE SYSTEM
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-12-01 至 2026-11-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10537089
• 关键词: 3-Dimensional; Acoustics; Adipose tissue; Adult; Adverse event; Animal Model; Animals; Autopsy; Bilateral; Cell Separation; Cells; Chemicals; Chronic; Cicatrix; Collagen Fiber; Communication impairment; Data; Data Collection; Deposition; Desmosine; Development; Elastic Fiber; Emotional; Excision; Extracellular Matrix; Family suidae; Fatigue; Female; Fibrin; Fibrosis; Frequencies; Gastroesophageal reflux disease; Goals; Head and Neck Cancer; Histologic; Human; Hydrogels; Hydroxyproline; Immunocompetent; Impairment; Implant; Implantation procedure; Inhalation; Injury; Laboratories; Laryngeal Diseases; Larynx; Laser Scanning Microscopy; Link; Malignant neoplasm of larynx; Mammals; Measurement; Measures; Mechanics; Methods; Miniature Swine; Modeling; Morphology; Occupational; Occupations; Operative Surgical Procedures; Oryctolagus cuniculus; Outcome; Periodicals; Phonation; Pilot Projects; Postoperative Period; Proteins; Proteomics; Radiation therapy; Recovery; Regenerative Medicine; Research; Safety; Sampling; Serum; Smoke; Social Interaction; Speed; Structure; Testing; Tissue Engineering; Tissues; Translations; Trauma; Urine; Veterans; Voice; Voice Disturbances; Voice Quality; Work; efficacy testing; experience; functional restoration; healing; implantation; improved; in vivo; injured; male; mechanical behavior; mechanical properties; mesenchymal stromal cell; multipotent cell; repaired; replacement tissue; restoration; scaffold; smoke inhalation; social; sound; tissue culture; vibration; vocal cord; wound healing

Veterans suffering loss of the larynx's vocal fold cover due to trauma or laryngeal cancer can suffer disabling voice difficulties, and treatment options are limited. A vibrating replacement tissue would revolutionize the treatment of laryngeal disorders. The work described in this proposal determines the long- term safety and efficacy of a tissue-engineered Cell-based Outer Vocal cord Replacement (COVR). Adult human multipotent cells isolated from adipose tissue are cultured within fibrin hydrogel to produce a three-dimensional tissue substitute. Upon implantation in rabbits, this construct healed well and produced normal short-term vibration for voicing. A large animal model is needed before human translation. Previous work has developed the implantation method in pigs. This proposed research will assess the long-term safety of the tissue-engineered implant up to 1 year after implant. Voice function will be studied to determine if the implant can restore normal voice quality after vocal cord injury. Completion of the proposed research will determine whether the COVR meets safety and efficacy requirements to proceed with FDA application for a human trial.

由于创伤或 喉癌可能会遇到残疾的语音困难，治疗选择是 有限的。振动的替换组织将彻底改变 喉疾病。该提案中描述的工作决定了长期 组织工程细胞的外声线的术语安全性和功效 替换（COVR）。从脂肪中分离出的成年人类多能细胞 在纤维蛋白水凝胶中培养组织以产生三维组织 代替。植入兔子后，该结构愈合得很好并产生 正常的短期振动。 在人类翻译之前需要大型动物模型。以前的工作 已经在猪中开发了植入方法。这项拟议的研究将 最多可在1年后评估组织工程植入物的长期安全性 注入。语音功能将被研究以确定植入物是否可以还原 声带受伤后的正常语音质量。拟议研究的完成 将确定COVR是否符合安全性和功效要求 继续进行FDA申请进行人体试验。