CYNOMOLGUS AND VERVET MONKEY DECIDUAL LYMPHOCYTE POPULATIONS

食蟹猴和黑长尾猴的蜕膜淋巴细胞群

• 批准号: 8173104
• 负责人: THADDEUS G GOLOS
• 金额: $ 3.1万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8173104
• 关键词: Animal Experimentation; Animal Model; Ascaridil; Biological Assay; CD8-Positive T-Lymphocytes; Cell Differentiation process; Cells; Centrifugation; Cercopithecus pygerythrus; Cercopithecus tantalus; Cesarean section; Chromium; Color; Computer Retrieval of Information on Scientific Projects Database; Decidua; Embryo; Endometrium; Environment; Evaluation; FCGR3B gene; Fetal Development; Flow Cytometry; Funding; Grant; Human; Immune; Immune response; Immunogenetics; Immunology; Implant; Institution; Leukocytes; Lymphocyte; MHC Class I Genes; Maternal-Fetal Exchange; Monkeys; NCAM1 gene; Natural Killer Cells; Peripheral; Phenotype; Population; Pregnancy; Primates; Proteins; Publications; Reporting; Research; Research Personnel; Resources; Services; Source; Staining method; Stains; Study models; T-Lymphocyte; United States National Institutes of Health; cell mediated lymphocytolysis test; cytotoxic; nonhuman primate; perforin; vervet; virology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: To determine the phenotypic and functional evaluation of decidual immune cells in the cynomolgus and vervet (African green) monkeys. The decidua is a specialized adaptation of the uterine endometrium to support the implanting embryo in primate pregnancy. Non-human primates are the important animal models for the study of maternal immune response within decidua to fetal development. Early pregnancy cynomologus and vervet deciduas were obtained by cesarean section. Lymphocytes were isolated by enzymatic dispersion and gradient centrifugation. Multi-color flow cytometry was used to phenotype cell populations, chromium release assay was used to evaluate cytotoxic activity of NK cells. NK cells were found as the richest lymphocyte population in the monkeys deciduas and the majority were CD56 bright cells, like human decidual NK (dNK) cells. Peripheral NK (pNK) cells in cynomolgus and vervet, in contrast to human, do not express CD56, suggesting the influence of a unique decidual environment to promote NK cell differentiation. While human CD56+ dNK cells are CD16-, more then 70% of monkeys' CD56+ dNK cells remained CD16+. Intracellular staining with anti-perforin mAbs demonstrated that dNK cells contain this protein. However, the cytotoxic potential was not apparent in chromium assay; CD56+ cells from cynomolgus decidua showed strongly reduced cytolytic activity against target cells when compared to pNK cells. Approximately 10% of decidual leukocytes are T cells. Most decidual T cells in cynomolgus and vervet were phenotyped as cytotoxic CD8+ cells (about 70%) and 2-15% co-expressed the NK cell marker CD56 (NKT cells). Together, these results showed that cynomolgus and vervet maternal-fetal interfaces are very rich in immune cells. Lymphocytes populations' diversity in monkeys' deciduas correlate with human studies, confirming that both species are excellent models for study mechanisms of immune recognition and tolerance that support primate pregnancy. This research used WNPRC Animal, Research, Assay, and Immunology & Virology Services. Funding ended before this reporting period began; publications have resulted. PUBLICATIONS: Bondarenko G.I., Dambaeva S.V., Grendell R.L., Hughes A.L., Durning, M., Garthwaite M.A., and Golos T.G. 2009. Characterization of cynomolgus and vervet monkey placental MHC class I expression: Diversity of the nonhuman primate AG locus. Immunogenetics 61:431-442. Dambaeva, S.V., Breburda, E.E., Durning, M., Garthwaite, M.A., Golos, G.G. 2009. Characterization of cynomolgus and vervet monkey decidual leukocyte populations. J. Reprod. Immunol. 80:57-69.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 目的：确定甲状腺菌和vervet（非洲绿色）猴子中判决免疫细胞的表型和功能评估。 DECIDUA是子宫子宫内膜的专门适应，以支持灵长类动物妊娠中的植入胚胎。 非人类灵长类动物是研究deciDUA对胎儿发育中母体免疫反应的重要动物模型。通过剖宫产获得了早期妊娠Cylomologus和Vervet Deciduas。 通过酶促分散和梯度离心分离淋巴细胞。 多色流式细胞仪用于表型细胞群体，铬释放测定法用于评估NK细胞的细胞毒性活性。 发现NK细胞是猴子deciduas中最富有的淋巴细胞种群，大多数是CD56明亮的细胞，例如人类deciDual NK（DNK）细胞。 与人类相比，cynomolgus和vervet中的外围NK（PNK）细胞没有表达CD56，这表明独特的deciDual环境的影响以促进NK细胞分化。 虽然人CD56+ DNK细胞为CD16-，而猴子的CD56+ DNK细胞中有70％保持CD16+。 用抗透明纤维mAb的细胞内染色表明DNK细胞含有该蛋白质。 但是，在铬测定中，细胞毒性潜力并不明显。与PNK细胞相比，来自cynomolgus decidua的CD56+细胞对靶细胞显示出大幅度降低的溶细胞活性。 约有10％的decIDual白细胞是T细胞。 将大多数cynomolgus和vervet中的de骨细胞表型为细胞毒性CD8+细胞（约70％），而2-15％共表达了NK细胞标记CD56（NKT细胞）。 总之，这些结果表明，cynomolgus和vervet母体媒体界面在免疫细胞中非常丰富。 淋巴细胞种群的“猴子的多样性”与人类的研究相关，证实这两种物种都是支持灵长类动物妊娠的免疫识别和耐受性研究机制的绝佳模型。 这项研究使用了WNPRC动物，研究，测定和免疫学和病毒学服务。在此报告期开始之前，资金结束了；出版物已导致。 出版物： Bondarenko G.I.，Dambaeva S.V.，Grendell R.L.，Hughes A.L.，Durning，M.，Garthwaite M.A.和Golos T.G. 2009。cynomolgus和vervet猴子胎盘MHC I类表达的表征：非人类灵长类动物AG基因座的多样性。 免疫遗传学61：431-442。 Dambaeva，S.V.，Breburda，E.E.，Durning，M.，Garthwaite，M.A.，Golos，G.G。 2009年。cynomolgus和vervet猴子deci核白细胞种群的表征。 J. 复制。免疫。 80：57-69。