MHC TYPING OF MACAQUES USED IN AIDS RESEARCH

用于艾滋病研究的猕猴 MHC 分型

• 批准号: 8173081
• 负责人: David I Watkins
• 金额: $ 5.16万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8173081
• 关键词: Alleles; American; Animal Model; Ascaridil; Binding; CD8B1 gene; Cell Line; Characteristics; Chinese People; Communities; Computer Retrieval of Information on Scientific Projects Database; European; Funding; Genes; Genetic Polymorphism; Grant; HIV vaccine; HIV-1; HLA-B 2705 antigen; Haplotypes; Histocompatibility Antigens Class I; Immunogenetics; Immunologic Deficiency Syndromes; Individual; Infection; Institution; Laboratories; MHC Class I Genes; Macaca; Macaca fascicularis; Macaca mulatta; Membrane; Microsatellite Repeats; Molecular; Pan Genus; Pathogenesis; Peptides; Principal Investigator; Pseudogenes; Recording of previous events; Research; Research Personnel; Resources; SIV; Services; Shivering; Source; T-Lymphocyte; Techniques; Technology; Testing; Training; Transcript; United States National Institutes of Health; Vaccines; Variant; Virus; Virus Replication; Work; bean; neutralizing monoclonal antibodies; response; simian human immunodeficiency virus; vaccine development; virology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: To offer MHC typing of the MHC class I and II loci for investigators working with macaques (Indian rhesus, Chinese rhesus, and Cynomolgus). To adapt technologies for HLA typing to molecular typing of macaque (Indian rhesus, Chinese rhesus, and Cynomolgus) MHC class I and II. The increased utility of various species of macaques as animal models in both HIV vaccine development and pathogenesis studies necessitates the continuation of reference MHC typing laboratories for these species. We plan to continue to offer services to both the North American and European scientific communities for MHC typing of macaques. Initially, this will include PCR-SSP tests for alleles encoding MHC class I and II molecules that bind peptides derived from SIV and SHIV. We are developing additional molecular techniques for analysis of the Indian rhesus and Chinese rhesus and Cynomolgus macaque MHC class I and class II alleles. Additionally, we offer training and materials to individual laboratories that wish to set up MHC typing. Finally, we are developing a panel of well-characterized cell lines that will be invaluable for the analysis of the MHC in the macaque. Since 2002 to the present, we have performed over 126,000 typings for over 130 investigators at 50 institutions. In 2009, we have conducted 21,800 typings at the request of 23 principal investigators from 19 different institutions. We are preparing to offer expanded Class II typing services during 2010. This research used WNPRC Immunogenetics & Virology Services. PUBLICATIONS: Doxiadis GG, Heijmans CM, Bonhomme M, Otting N, Crouau-Roy B, Bontrop RE. Compound evolutionary history of the rhesus macaque MHC class I B region revealed by microsatellite analysis and localization of retroviral sequences. PLoS One. 2009;4(1):e4287. PMID: 19172173 Wilson NA, Keele BF, Reed JS, Piaskowski SM, MacNair CE, Bett AJ, Liang X, Wang F, Thoryk E, Heidecker GJ, Citron MP, Huang L, Lin J, Vitelli S, Ahn CD, Kaizu M, Maness NJ, Reynolds MR, Friedrich TC, Loffredo JT, Rakasz EG, Erickson S, Allison DB, Piatak M Jr, Lifson JD, Shiver JW, Casimiro DR, Shaw GM, Hahn BH, Watkins DI. Vaccine-induced cellular responses control simian immunodeficiency virus replication after heterologous challenge. J Virol. 2009 Jul;83(13):6508-21. PMID: 19403685 Loffredo JT, Sidney J, Bean AT, Beal DR, Bardet W, Wahl A, Hawkins OE, Piaskowski S, Wilson NA, Hildebrand WH, Watkins DI, Sette A. Two MHC class I molecules associated with elite control of immunodeficiency virus replication, Mamu-B*08 and HLA-B*2705, bind peptides with sequence similarity. J Immunol. 2009 Jun 15;182(12):7763-75. PMID: 19494300 Valentine LE, Loffredo JT, Bean AT, Le¿n EJ, MacNair CE, Beal DR, Piaskowski SM, Klimentidis YC, Lank SM, Wiseman RW, Weinfurter JT, May GE, Rakasz EG, Wilson NA, Friedrich TC, O'Connor DH, Allison DB, Watkins DI. Infection with "escaped" virus variants impairs control of simian immunodeficiency virus SIVmac239 replication in Mamu-B*08-positive macaques. J Virol. 2009 Nov;83(22):11514-27. Sep 2.PMID: 19726517 Vojnov L, Reed JS, Weisgrau KL, Rakasz EG, Loffredo JT, Piaskowski SM, Sacha JB, Kolar HL, Wilson NA, Johnson RP, Watkins DI. Effective simian immunodeficiency virus-specific CD8+ T cells lack an easily detectable, shared characteristic. J Virol. 2010 Jan;84(2):753-64.PMID: 19889785 Otting N, Doxiadis GG, Bontrop RE. Definition of Mafa-A and -B haplotypes in pedigreed cynomolgus macaques (Macaca fascicularis). Immunogenetics. 2009 Nov 24. PMID: 19937015 de Groot NG, Heijmans CM, de Groot N, Doxiadis GG, Otting N, Bontrop RE. The chimpanzee Mhc-DRB region revisited: gene content, polymorphism, pseudogenes, and transcripts. Mol Immunol. 2009 Dec;47(2-3):381-9. PMID: 19800692 Hessell AJ, Rakasz EG, Tehrani DM, Huber M, Weisgrau KL, Landucci G, Forthal DN, Koff WC, Poignard P, Watkins DI, Burton DR. Broadly neutralizing monoclonal antibodies 2F5 and 4E10 directed against the human immunodeficiency virus type 1 gp41 membrane-proximal external region protect against mucosal challenge by simian-human immunodeficiency virus SHIVBa-L. J Virol. 2010 Feb;84(3):1302-13. PMID: 19906907

该副本是使用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这是调查员的机构。 目的：提供MHC I类和II级本地的MHC键入，以供使用Macaques（印度恒河猴，中国恒河猴和cynomolgus）工作的调查人员。适应HLA键入的技术，以猕猴（印度恒河猴，中国恒河猴和cynomolgus）MHC I类和II类的分子键入。 在艾滋病毒疫苗发育和发病机理研究中，各种猕猴作为动物模型的效用越来越多，以继续向北美和欧洲科学群落提供服务，以提供猕猴的MHC键入。最初，这将包括编码MHC I和II类分子的等位基因的PCR-SSP测试，这些分子结合了源自SIV和SHIV的肽。我们正在开发其他分子技术，用于分析印度恒河猴，中国恒河猴以及甲状腺猕猴MHC I和II类等位基因。此外，我们为希望设置MHC打字的个别实验室提供培训和材料。最后，我们正在开发一组良好的细胞系，对于猕猴中的MHC分析将是无价的。自2002年以来，我们已经为50个机构的130多名调查人员进行了126,000多种键入。 2009年，我们应19个不同机构的23位首席调查人员的要求进行了21,800次打字。我们准备在2010年提供扩展的II类打字服务。 这项研究使用了WNPRC免疫原性和病毒学服务。 出版物： Doxiadis GG，Heijmans CM，Bonhomme M，Otting N，Crouau-Roy B，Bontrop RE。微卫星分析和逆转录病毒序列的定位揭示了恒河猕猴MHC I类区域的复合进化史。 PLOS一个。 2009; 4（1）：E4287。 PMID：19172173 Wilson NA, Keele BF, Reed JS, Piaskowski SM, MacNair CE, Bett AJ, Liang X, Wang F, Thoryk E, Heidecker GJ, Citron MP, Huang L, Lin J, Vitelli S, Ahn CD, Kaizu M, Maness NJ, Reynolds MR, Friedrich TC, Loffredo JT, Rakasz EG, Erickson S，Allison DB，Piatak M JR，Lifson JD，Shiver JW，Casimiro DR，Shaw GM，Hahn BH，Watkins DI。疫苗诱导的细胞反应控制异源挑战后的邻苯二甲酸免疫缺陷病毒复制。 J Virol。 2009年7月； 83（13）：6508-21。 PMID：19403685 Loffredo JT，Sidney J，Bean AT，Bardet W，Bardet W，Wahl A，Hawkins OE，Piaskowski S，Wilson NA，Hildebrand WH，Watkins DI，Sette A.两个MHC I类分子与具有免疫缺陷病毒复制的精英控制相关的MHC I类分子，MAMU-BINDINES，MAMU-B*08和HLA，HLA和HLA，HLA*2705。 J免疫。 2009年6月15日； 182（12）：7763-75。 PMID：19494300 Valentine LE，Loffredo JT，Bean AT，Le¿n EJ，Macnair CE，Beal DR，Piaskowski SM，Klimentdis YC，Lank SM，Wiseman RW，Weinfurter JT，May GE，Rakasz EG，Rakasz EG，Wilson Na，Wilson Na，Friedrich TC，Friedrich TC，O'Connor DH，O'Connor DH，Allison DB，WALSISON DI。用“逃脱”病毒变体的感染会损害MAMU-B*08阳性猕猴中猿猴免疫缺陷病毒SIVMAC239复制的控制。 J Virol。 2009年11月； 83（22）：11514-27。 9月2日。PMID：19726517 Vojnov L，Reed JS，Weisgrau KL，Rakasz EG，Loffredo JT，Piaskowski SM，Sacha JB，Kolar HL，Wilson NA，Wilson NA，Johnson RP，Watkins DI。有效的猿猴免疫缺陷特异性CD8+ T细胞缺乏易于检测的，共同的特征。 J Virol。 2010年1月； 84（2）：753-64.PMID：19889785 Otting N，Doxiadis GG，Bontrop Re。 MAFA -A和-B单倍型的定义在谱系的Cynomolgus猕猴（Macaca fascicularis）中的定义。免疫遗传学。 2009年11月24日。PMID：19937015 De Groot Ng，Heijmans CM，De Groot N，Doxiadis GG，Otting N，Bontrop Re。 三支黑猩猩MHC-DRB区域进行了修订：基因含量，多态性，假基因和转录本。摩尔免疫。 2009年12月; 47（2-3）：381-9。 PMID：19800692 Hessell AJ，Rakasz EG，Tehrani DM，Huber M，Weisgrau KL，Landucci G，Fortal DN，Koff WC，Poignard P，Watkins DI，Burton Dr。针对人类免疫缺陷病毒1型GP41膜膜外部区域的广泛中和单克隆抗体2F5和4E10可预防拟妇合 - 人类免疫缺陷病毒Shivba-Shivba-Shivba-L。 J Virol。 2010年2月； 84（3）：1302-13。 PMID：19906907