Yellow Fever, rDNA (EP+IL-12) and rAd35 as Vectors for AIDS Vaccine Development

黄热病、rDNA (EP IL-12) 和 rAd35 作为艾滋病疫苗开发的载体

基本信息

批准号：
8874851
负责人：
David I Watkins
金额：
$ 195.69万
依托单位：
UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-07-01 至 2016-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8874851
关键词：
AIDS vaccine development Acute Address Adenoviruses Animals Area Attenuated Chronic Phase Clinical Trials Control Animal Cytomegalovirus Data Dose Electroporation Figs - dietary Gagging Genes Genome HIV HIV vaccine Human Immune response Immunity Immunization Intention Interleukin-12 Macaca Macaca mulatta Methods Muscle NIH Program Announcements Open Reading Frames Outcome Phase Plasmids Proteins Proteome RNA Recombinant DNA Recombinant Interleukin-12 Recombinants Regimen Research Role SIV Series Serotyping T cell response Testing Tissues Vaccinated Vaccination Vaccine Design Vaccines Viral Viral Load result Viremia Virus Virus Replication Work Yellow Fever Yellow Fever Vaccine design improved lymph nodes nonhuman primate novel vaccines plasmid DNA prevent public health relevance rectal research study vaccine development vaccine trial vector

项目摘要

DESCRIPTION (provided by applicant): We plan to explore whether the highly successful attenuated yellow fever (YF-17D) vaccine, rDNA delivered by electroporation along with IL-12 and rAd35 can be used as vectors to express SIV proteins in a prime/boost regimen for vaccination against the AIDS virus. In a highly integrated series of experiments, we will first define the best viral targets for a vaccine. We will then bring the results together in a final efficacy experiment to test the vectors that, if successful, could prevent suffering by millions of people worldwide. Our application seeks to address four of the ten research areas outlined in the HIVRAD program announcement. Our project has the potential to impact HIV vaccine development on two fronts. First, we will define the targets of a successful vaccine in a challenge experiment. This will allow us to design a vaccine encoding the critical targets of a successful immune response. Second, we will develop three entirely novel vaccine vectors. If we are successful in this endeavor, our results will have a profound effect on HIV vaccine design.

描述（由申请人提供）：我们计划探讨是否可以将非常成功的减毒黄热病（YF-17D）疫苗，通过电穿孔和IL-12和RAD35送达的rDNA，可以用作载体，以在素/增强方案中表达SIV蛋白，以抗AIDS病毒的prime/boost方案进行疫苗接种。在一系列高度整合的实验中，我们将首先定义疫苗的最佳病毒靶标。然后，我们将在最终功效实验中将结果汇总在一起，以测试媒介，如果成功，可以防止数百万全球人。我们的申请旨在解决Hivrad计划公告中概述的十个研究领域中的四个。我们的项目有可能影响两个方面的艾滋病毒疫苗开发。首先，我们将在挑战实验中定义成功疫苗的靶标。这将使我们能够设计一种编码成功免疫反应的关键目标的疫苗。其次，我们将开发三个完全新颖的疫苗向量。如果我们在这项工作中取得成功，我们的结果将对HIV疫苗设计产生深远的影响。