TIME-RESOLVED X-RAY DIFFRACTION OF CARDIAC MUSCLE

心肌的时间分辨 X 射线衍射

• 批准号: 8168608
• 负责人: Pieter P. de TOMBE
• 金额: $ 1.44万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168608
• 关键词: Adrenergic Agents; Cardiac Volume; Computer Retrieval of Information on Scientific Projects Database; Cyclic AMP-Dependent Protein Kinases; Dependence; Development; Filament; Funding; Goals; Grant; Heart; Institution; Isoproterenol; Length; Microfilaments; Muscle; Myocardium; Operating System; Phosphorylation; Research; Research Personnel; Research Project Grants; Resources; Skeletal Muscle; Source; Starling (law); Sturnus vulgaris; Testing; Time; United States National Institutes of Health; Variant; adrenergic; base; in vivo

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Frank-Starling law of the heart describes the interrelationship between end-diastolic volume and cardiac ejection volume, a regulatory system that operates on a beat to beat basis. The overall goal of this research is to elucidate the structural mechanism(s) that underlie myofilament length dependent activation, the cellular mechanism responsible for Frank-Starling. To explore this hypothesis, the objective of the proposed research project is focused on answering the following questions that leads to two specific aims: 1) Is inter-filament spacing prior to active contraction the physiologically relevant parameter that determines myofilament Ca2+ responsiveness? It has been known for some time that active force development in skeletal muscle is associated with a change in inter-filament spacing AIM 1: to test the hypothesis that variation in inter-filament spacing with SL in active muscle underlies myofilament length dependent activation; furthermore determine under conditions of constant SL whether lattice spacing (LS) varies during activation, or remains constant as would be predicted by constant volume. Aim 2: Using isoproterenol, a compound that activates PKA phosphorylation, which has been shown to alter diastolic in vivo LS, test the dependence of LS during ¿-adrenergic stimulation

该副本是使用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。主题项目和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这是调查员的机构。 心脏的坦率宣传法描述了末期舒适量和心脏弹出卷之间的相互关系，这是一种调节系统，以节拍为基础。这项研究的总体目的是阐明肌丝长度依赖性激活的结构机制，这是导致坦率的细胞机制。为了探讨这一假设，拟议的研究项目的目的是回答导致两个特定目标的以下问题：1）在主动收缩之前是丝间间距的间隔，在积极的物理相关参数上决定了肌丝Ca2+响应能力？一段时间以来，骨骼肌中的主动力发育与丝间间距的变化有关1：测试假设的假设是，活性肌肉中SL的丝间间距的变化是肌丝长度依赖性激活的基础；此外，在恒定SL的条件下确定晶格间距（LS）在激活过程中是否会有所不同，还是像恒定体积所预测的那样保持恒定。 AIM 2：使用异丙肾上腺素，一种激活PKA磷酸化的化合物，已证明可以改变体内舒张期LS，测试LS在 - 肾上腺肾上腺刺激过程中的依赖性