IMAGING LUNG CANCER IN MICE TO IMPROVE RADIATION THERAPY

对小鼠肺癌进行成像以改善放射治疗

• 批准号: 8171581
• 负责人: David Guy Kirsch
• 金额: $ 2.18万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171581
• 关键词: Animals; Blood; Blood Vessels; Cardiac; Computer Retrieval of Information on Scientific Projects Database; Data; Detection; Electrocardiogram; Environmental air flow; Funding; Future; Goals; Gold; Grant; Histologic; IACUC; Image; Institution; Malignant neoplasm of lung; Methods; Modeling; Monitor; Mus; Physiologic pulse; Protocols documentation; Radiation therapy; Research; Research Personnel; Resolution; Resources; Scanning; Sensitivity and Specificity; Source; Stress; Testing; Tumor Volume; United States National Institutes of Health; Work; density; improved; respiratory; skills; tumor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Explore the utility of microCT as a quantiative marker for lung cancer. As part of an ongoing K08 grant we will be studying the efficacy of a number of therapies in this model of primary lung cancer, with these goals: Specific Aim 1- this project will help establish the logistical support required for the extended studies in support of this K08. More specifically the project will serve as a vehicle to work out animal transfer and IACUC for future studies. This will also provide a vehicle to streamline the gating protocols. Currently animals are intubated for scan synchronous ventilation and monitored with ECG. We propose the use of a pulse oximeter as a less invasive method that will streamline the acquisition. Specific Aim 2- previous work @ MGH has gathered reasonable image data without cardiac and respiratory gating. Our hypothesis is that the absence of this support leads to overestimation of tumor volumes. By comparing the acquisition with and without gating, we will determine specifically the impact of gating on tumor volume. This is a survival protocol and will serve as a vehicle to stress our survival support skills. Specific Aim 3 - Histologic evidence suggests that these tumors will have vascular densities of ~15% of volume. We hypothesize that a dual scan (with/without blood pool agent) will allow us to quantitate vascular density and changes in that density. This project will test that hypothesis and lay the groundwork for an R01 application. Specific Aim 4 - to this point , microPET has been considered a gold standard for detection of small tumors. We hypothesis that for the mouse, the resolution of the microCT might be sufficiently high to provide an increased level of sensitivity (and specificity) beyond that of the current gold standard. This too will provide support for a planned R01 application.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 探索 microCT 作为肺癌定量标志物的效用。作为正在进行的 K08 资助的一部分，我们将研究多种疗法在这种原发性肺癌模型中的疗效，目标如下： 具体目标 1 - 该项目将帮助建立支持本 K08 的扩展研究所需的后勤支持。更具体地说，该项目将作为未来研究的动物转移和 IACUC 的工具。这也将提供一种简化门控协议的工具。目前，对动物进行插管以进行扫描同步通气并用心电图进行监测。我们建议使用脉搏血氧计作为一种侵入性较小的方法，以简化采集过程。 具体目标 2 - MGH 之前的工作已经收集了合理的图像数据，无需心脏和呼吸门控。我们的假设是，缺乏这种支持会导致对肿瘤体积的高估。通过比较有门控和无门控的采集，我们将具体确定门控对肿瘤体积的影响。这是一个生存协议，将作为强调我们生存支持技能的工具。 具体目标 3 - 组织学证据表明这些肿瘤的血管密度约为体积的 15%。我们假设双重扫描（有/没有血池剂）将使我们能够定量血管密度和该密度的变化。该项目将测试该假设并为 R01 应用奠定基础。 具体目标 4 - 到目前为止，microPET 已被认为是检测小肿瘤的金标准。我们假设对于小鼠来说，microCT 的分辨率可能足够高，可以提供超出当前金标准的更高水平的灵敏度（和特异性）。这也将为计划中的 R01 应用提供支持。