IMAGING LUNG CANCER IN MICE TO IMPROVE RADIATION THERAPY

对小鼠肺癌进行成像以改善放射治疗

• 批准号: 8171581
• 负责人: David Guy Kirsch
• 金额: $ 2.18万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171581
• 关键词: Animals; Blood; Blood Vessels; Cardiac; Computer Retrieval of Information on Scientific Projects Database; Data; Detection; Electrocardiogram; Environmental air flow; Funding; Future; Goals; Gold; Grant; Histologic; IACUC; Image; Institution; Malignant neoplasm of lung; Methods; Modeling; Monitor; Mus; Physiologic pulse; Protocols documentation; Radiation therapy; Research; Research Personnel; Resolution; Resources; Scanning; Sensitivity and Specificity; Source; Stress; Testing; Tumor Volume; United States National Institutes of Health; Work; density; improved; respiratory; skills; tumor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Explore the utility of microCT as a quantiative marker for lung cancer. As part of an ongoing K08 grant we will be studying the efficacy of a number of therapies in this model of primary lung cancer, with these goals: Specific Aim 1- this project will help establish the logistical support required for the extended studies in support of this K08. More specifically the project will serve as a vehicle to work out animal transfer and IACUC for future studies. This will also provide a vehicle to streamline the gating protocols. Currently animals are intubated for scan synchronous ventilation and monitored with ECG. We propose the use of a pulse oximeter as a less invasive method that will streamline the acquisition. Specific Aim 2- previous work @ MGH has gathered reasonable image data without cardiac and respiratory gating. Our hypothesis is that the absence of this support leads to overestimation of tumor volumes. By comparing the acquisition with and without gating, we will determine specifically the impact of gating on tumor volume. This is a survival protocol and will serve as a vehicle to stress our survival support skills. Specific Aim 3 - Histologic evidence suggests that these tumors will have vascular densities of ~15% of volume. We hypothesize that a dual scan (with/without blood pool agent) will allow us to quantitate vascular density and changes in that density. This project will test that hypothesis and lay the groundwork for an R01 application. Specific Aim 4 - to this point , microPET has been considered a gold standard for detection of small tumors. We hypothesis that for the mouse, the resolution of the microCT might be sufficiently high to provide an increased level of sensitivity (and specificity) beyond that of the current gold standard. This too will provide support for a planned R01 application.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 探索MicroCT作为肺癌的定量标记的效用。作为正在进行的K08赠款的一部分，我们将研究这种原发性肺癌模型中多种疗法的功效，并具有这些目标： 具体目标1-该项目将有助于建立扩展研究所需的后勤支持，以支持该K08。更具体地说，该项目将作为制定动物转移和IACUC的工具。这还将提供简化门控协议的工具。目前，动物插管进行扫描同步通风，并用ECG进行监测。我们提出使用脉搏血氧仪作为一种侵入性的方法，该方法将简化采集。 特定目标2-先前的工作 @ MGH已收集了没有心脏和呼吸门控的合理图像数据。我们的假设是缺乏这种支持会导致高估肿瘤体积。通过比较带有和没有门控的获取，我们将特别确定门控对肿瘤体积的影响。这是一种生存方案，将作为强调我们生存支持技能的工具。 特定目的3-组织学证据表明，这些肿瘤的血管密度约为量的15％。我们假设双重扫描（带有/没有血池剂）将使我们能够定量血管密度和该密度的变化。该项目将检验该假设，并为R01应用程序奠定基础。 具体目标4-到这一点，Micropet被认为是检测小肿瘤的金标准。我们假设对于小鼠，微观的分辨率可能足够高，可以超出当前黄金标准的敏感性（和特异性）水平。这也将为计划的R01应用程序提供支持。