COPPER HIERARCHY IN ZEBRAFISH

斑马鱼的铜层次结构

• 批准号: 8168662
• 负责人: ANIRUDDHA DEB
• 金额: $ 4.32万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168662
• 关键词: Affect; Biology; Cells; Chelating Agents; Computer Retrieval of Information on Scientific Projects Database; Copper; Detection; Development; Embryo; Funding; Gene Mutation; Grant; Homeostasis; Image; Indium; Institution; Ions; Maps; Metals; Neurologic; Physiology; Pigmentation physiologic function; Play; Process; Proteins; Research; Research Personnel; Resources; Role; Source; System; Tissues; Trace metal; United States National Institutes of Health; Zebrafish; design; hypocupremia; notochord development; trafficking; Affect; Biology; Cells; Chelating Agents; Computer Retrieval of Information on Scientific Projects Database; Copper; Detection; Development; Embryo; Funding; Gene Mutation; Grant; Homeostasis; Image; Indium; Institution; Ions; Maps; Metals; Neurologic; Physiology; Pigmentation physiologic function; Play; Process; Proteins; Research; Research Personnel; Resources; Role; Source; System; Tissues; Trace metal; United States National Institutes of Health; Zebrafish; design; hypocupremia; notochord development; trafficking

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Trace metal ions play a critical role in biology. One critical aspect of understanding the inorganic physiology of trace metals is detection of the distribution of the elements in tissues. In this proposal we will be utilising XRF imaging to study the metal homeostasis. Zebrafish embryos with increasing levels of copper deficiency have been designed using genetic mutation and copper chelators. As copper levels decrease, non-essential copper dependent processes, such as pigmentation, are lost. Then notochord development is affected and finally neurological deformation. A copper hierarchy has been identified where copper is target to more essential systems first and then to non-essential systems. It is unknown whether this hierarchy is enforced on a systemic level or a cellular level, is copper targeted to the cells responsible for neurological development over those responsible for pigmentation, or is copper delivery equal in all cells but the threshold cellular copper levels need to express the proteins responsible for pigmentation higher that those needed for expression of more essential proteins. XRF imaging will be used to study the distribution of copper in Zebrafish embryos to map the system wide trafficking of this metal.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 痕量金属离子在生物学中起关键作用。理解痕量金属无机生理的一个关键方面是检测组织中元素的分布。在此提案中，我们将利用XRF成像研究金属稳态。斑马鱼胚胎具有铜缺乏水平的升高，已使用基因突变和铜螯合剂设计。 随着铜水平的降低，诸如色素沉着之类的非必需铜依赖性过程将丢失。 然后，脊索发育受到影响，最后神经变形。 已经确定了铜层次结构，即铜是首先针对更重要的系统，然后是非必需系统的目标。 尚不清楚该层次结构是在全身水平还是细胞水平上执行的，是针对负责色素沉着的细胞的细胞的铜，还是所有细胞中的铜递送相等，但阈值细胞铜水平需要表达蛋白质的蛋白质，使蛋白质表达较高的蛋白质所需的蛋白质，以表达更重要的蛋白质表达的蛋白质。 XRF成像将用于研究斑马鱼胚胎中铜的分布，以绘制该金属的广泛运输系统。