IMAGING NANOPARTICLES FOR DIAGNOSTIC & THERAPEUTIC USE IN MOUSE TISSUES BY XFM

纳米颗粒成像用于诊断

• 批准号: 8168624
• 负责人: GAYLE E. WOLOSCHAK
• 金额: $ 1.62万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168624
• 关键词: Area; Biochemical; Biological; Cobalt; Computer Retrieval of Information on Scientific Projects Database; Cryoultramicrotomy; Cultured Cells; Diagnostic; Elements; Fluorescence; Freezing; Funding; Goals; Gold; Grant; Image; Institution; Iron; Maps; Mus; Nature; Phosphorus; Property; Research; Research Personnel; Resources; Roentgen Rays; Signal Transduction; Source; Surface; Testing; Therapeutic; Therapeutic Uses; Tissues; Titanium; Tumor Tissue; United States National Institutes of Health; beamline; in vivo; nano; nanocomposite; nanoparticle; novel; research study; uptake

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this project is to test the feasibility of TiO2 and derivative nanoparticles (Co-Fe-TiO2 and Co-Fe-Au-TiO2) nanoparticles and nanoparticle bio-organic molecule nanocomposites as diagnostic and/or therapeutic vehicles. Our previous experiments in cultured cells have shown that TiO2-DNA nanocomposites have unique and novel biochemical properties that would be very suitable for biomedical uses (Paunesku, et al.. Nature Materials (2003) 2 (5):343-346). We have since developed several new nanoparticles, and we investigated different ways to induce cellular uptake of such nanoparticles/nanocomposites (Figure 1). Therefore, we are now poised to test these different nano-constructs in vivo, in mice. Our overall goals can be organized in two aims: Aim 1) Testing different nanoparticle derivatives of TiO2 for in vivo stability and distribution Aim 2) Testing different nanoparticle coatings for in vivo stability and establishing their effects on in vivo distribution. At the BioCAT beamline we are investigating presence of titanium, cobalt, iron, gold and relevant "biological" elements: P, S, Ca, Zn using X-ray fluorescence in different mouse tissue sections with large surface area. We inject the nanocomposites directly into tumor tissue or intravenously or intraperitoneally. We subsequently isolate different tissues, freeze and cryosection them. We look for overlap of Ti signal with fluorescence signals of other elements present in nanoparticles and map their presence in different areas of tissue by comparing these signals with the signals produced by P, S, Zn and other elements "naturally" present in tissues.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 The purpose of this project is to test the feasibility of TiO2 and derivative nanoparticles (Co-Fe-TiO2 and Co-Fe-Au-TiO2) nanoparticles and nanoparticle bio-organic molecule nanocomposites as diagnostic and/or therapeutic vehicles. 我们以前在培养细胞中的实验表明，TiO2-DNA纳米复合材料具有独特而新颖的生化特性，非常适合生物医学用途（Paunesku等。自然材料（2003）2（5）：343-346）。 此后，我们已经开发了几种新的纳米颗粒，并研究了不同的方法来诱导这种纳米颗粒/纳米复合材料的细胞摄取（图1）。 因此，我们现在准备在小鼠中测试体内这些不同的纳米构造。 Our overall goals can be organized in two aims: Aim 1) Testing different nanoparticle derivatives of TiO2 for in vivo stability and distribution Aim 2) Testing different nanoparticle coatings for in vivo stability and establishing their effects on in vivo distribution.在BioCat光束线上，我们正在研究钛，钴，铁，金和相关的“生物”元素的存在：P，S，Ca，Zn使用X射线荧光在不同的小鼠组织切片中具有较大的表面积。 我们将纳米复合材料直接注入肿瘤组织或静脉内或腹膜内。 随后，我们将不同的组织分离，冷冻和冷冻切除。 We look for overlap of Ti signal with fluorescence signals of other elements present in nanoparticles and map their presence in different areas of tissue by comparing these signals with the signals produced by P, S, Zn and other elements "naturally" present in tissues.