IMAGING NANOPARTICLES FOR DIAGNOSTIC & THERAPEUTIC USE IN MOUSE TISSUES BY XFM

纳米颗粒成像用于诊断

• 批准号: 8168624
• 负责人: GAYLE E. WOLOSCHAK
• 金额: $ 1.62万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168624
• 关键词: Area; Biochemical; Biological; Cobalt; Computer Retrieval of Information on Scientific Projects Database; Cryoultramicrotomy; Cultured Cells; Diagnostic; Elements; Fluorescence; Freezing; Funding; Goals; Gold; Grant; Image; Institution; Iron; Maps; Mus; Nature; Phosphorus; Property; Research; Research Personnel; Resources; Roentgen Rays; Signal Transduction; Source; Surface; Testing; Therapeutic; Therapeutic Uses; Tissues; Titanium; Tumor Tissue; United States National Institutes of Health; beamline; in vivo; nano; nanocomposite; nanoparticle; novel; research study; uptake

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this project is to test the feasibility of TiO2 and derivative nanoparticles (Co-Fe-TiO2 and Co-Fe-Au-TiO2) nanoparticles and nanoparticle bio-organic molecule nanocomposites as diagnostic and/or therapeutic vehicles. Our previous experiments in cultured cells have shown that TiO2-DNA nanocomposites have unique and novel biochemical properties that would be very suitable for biomedical uses (Paunesku, et al.. Nature Materials (2003) 2 (5):343-346). We have since developed several new nanoparticles, and we investigated different ways to induce cellular uptake of such nanoparticles/nanocomposites (Figure 1). Therefore, we are now poised to test these different nano-constructs in vivo, in mice. Our overall goals can be organized in two aims: Aim 1) Testing different nanoparticle derivatives of TiO2 for in vivo stability and distribution Aim 2) Testing different nanoparticle coatings for in vivo stability and establishing their effects on in vivo distribution. At the BioCAT beamline we are investigating presence of titanium, cobalt, iron, gold and relevant "biological" elements: P, S, Ca, Zn using X-ray fluorescence in different mouse tissue sections with large surface area. We inject the nanocomposites directly into tumor tissue or intravenously or intraperitoneally. We subsequently isolate different tissues, freeze and cryosection them. We look for overlap of Ti signal with fluorescence signals of other elements present in nanoparticles and map their presence in different areas of tissue by comparing these signals with the signals produced by P, S, Zn and other elements "naturally" present in tissues.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 该项目的目的是测试 TiO2 及其衍生纳米颗粒（Co-Fe-TiO2 和 Co-Fe-Au-TiO2）纳米颗粒和纳米颗粒生物有机分子纳米复合材料作为诊断和/或治疗载体的可行性。 我们之前在培养细胞中的实验表明，TiO2-DNA 纳米复合材料具有独特且新颖的生化特性，非常适合生物医学用途（Paunesku 等人. Nature Materials (2003) 2 (5):343-346）。 此后，我们开发了几种新的纳米颗粒，并研究了诱导细胞摄取此类纳米颗粒/纳米复合材料的不同方法（图 1）。 因此，我们现在准备在小鼠体内测试这些不同的纳米结构。 我们的总体目标可以分为两个目标： 目标 1) 测试 TiO2 不同纳米粒子衍生物的体内稳定性和分布 目标 2) 测试不同纳米粒子涂层的体内稳定性并确定其对体内分布的影响。在 BioCAT 光束线，我们正在使用 X 射线荧光在具有大表面积的不同小鼠组织切片中研究钛、钴、铁、金和相关“生物”元素：P、S、Ca、Zn 的存在。 我们将纳米复合材料直接注射到肿瘤组织中或静脉内或腹膜内注射。 随后我们分离不同的组织，将其冷冻并冷冻切片。 我们寻找 Ti 信号与纳米粒子中存在的其他元素的荧光信号的重叠，并通过将这些信号与组织中“自然”存在的 P、S、Zn 和其他元素产生的信号进行比较，绘制它们在组织不同区域的存在情况。