HIGH-RESOLUTION EX VIVO IMAGING OF AN ALZHEIMER'S MOUSE MODEL

阿尔茨海默病小鼠模型的高分辨率离体成像

• 批准号: 8171559
• 负责人: JOANNA L JANKOWSKY
• 金额: $ 1.09万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171559
• 关键词: Alzheimer' s Disease; Amyloid deposition; Amyloidosis; Animals; Area; Atlases; Brain; Collaborations; Complement; Computer Retrieval of Information on Scientific Projects Database; Data; Data Analyses; Disease; Funding; Gene Expression; Goals; Grant; Image; Institution; Magnetic Resonance Imaging; Molecular Profiling; Mus; Neurodegenerative Disorders; Research; Research Personnel; Resolution; Resources; Source; Testing; Tetanus Helper Peptide; Time; United States National Institutes of Health; Work; mouse model

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Using MR 9T magnet for this study. The MouseBIRN collaborative group seeks testbed cases to generate a searchable, online atlas that combines gene expression and 3D MRI images of mouse models for neurodegenerative disease. We would like to develop the new tet-off APP mouse model of Alzheimer's disease in this context. Work is already under way to generate gene expression profiles of the tet-off APP mice at multiple points in the progression of amyloidosis. The imaging proposed in collaboration with CIVM would provide comprehensive, high-resolution, 3D morphological images to complement the gene expression analyses. We will focus imaging efforts on two of the five time points assessed for gene expression, one before and one after the onset of amyloid deposition. The goal is to develop an average rendering from 5 Alzheimer's (tet-off APP) and 5 control (tTA) animals at each time point that can be used not only as a test of the MouseBIRN automated data analysis pipeline but also provide valuable information about volumetric and/or structural changes in specific brain areas as the disease progresses. Data from this study will be made publicly available through the MouseBIRN network.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 使用MR 9T磁铁进行这项研究。 Mousebirn协作小组寻求测试案例，以生成可搜索的在线地图集，该图案结合了基因表达和小鼠模型的神经退行性疾病模型的3D MRI图像。 在这种情况下，我们想开发新的Tet-Owt App小鼠模型。 在淀粉样变性进展中，在多个点生成Tet-Off App小鼠的基因表达谱的工作已经在进行中。 与CIVM合作提出的成像将提供全面，高分辨率的3D形态图像，以补充基因表达分析。 我们将将成像工作集中在评估基因表达的五个时间点中的两个，一个淀粉样蛋白沉积发作之前和一个。 目的是在每个时间点开发一个平均渲染，并在每个时间点开发5个控制动物（TTA）动物，不仅可以用作Mousebirn自动数据分析管道的测试，而且还提供了有关有价值的信息。随着疾病的发展，特定大脑区域的体积和/或结构变化。这项研究的数据将通过MouseBirn网络公开获得。