HEARING IN MICE AND CHICKENS

小鼠和鸡的听力

• 批准号: 8171351
• 负责人: Edwin W Rubel
• 金额: $ 0.2万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171351
• 关键词: Biological; Cessation of life; Chickens; Cochlear nucleus; Computer Retrieval of Information on Scientific Projects Database; Funding; Genes; Genetic Transcription; Grant; Hearing; Institution; Lead; Methods; Microarray Analysis; Mus; Neonatal; Neurons; Peripheral; Predisposition; Research; Research Personnel; Resources; Signal Pathway; Source; Synapses; United States National Institutes of Health; critical period; deprivation; fascinate; genetic analysis; mRNA Expression; mature animal; postnatal; response; Biological; Cessation of life; Chickens; Cochlear nucleus; Computer Retrieval of Information on Scientific Projects Database; Funding; Genes; Genetic Transcription; Grant; Hearing; Institution; Lead; Methods; Microarray Analysis; Mus; Neonatal; Neurons; Peripheral; Predisposition; Research; Research Personnel; Resources; Signal Pathway; Source; Synapses; United States National Institutes of Health; critical period; deprivation; fascinate; genetic analysis; mRNA Expression; mature animal; postnatal; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We will continue investigating the intercellular and intracellular signaling pathways that lead to neuronal death (or survival) following the elimination of excitatory synaptic input to the mammalian cochlear nucleus. A major initiative during the current grant period has been to use modern genetic analysis methods to begin assessing the biological mechanisms underlying differential responses of neonatal and adult animals to afferent deprivation (a critical period) of the mouse cochlear nucleus. Gene microarray technology was used to screen for genes that are differentially constitutively expressed in the cochlear nucleus during periods of differing susceptibility to peripheral deprivation and genes that are differentially regulated during vs. after this critical period in the first 48 hrs following peripheral deprivation (Harris et al., 2005; 2006; 2008). One of the most fascinating and important findings of these studies was that there is an enormous change in transcription during the transition between afferent dependent neuron survival (P2-P11; P=postnatal day) and afferent independent survival (>P14), which corresponds closely with the onset of hearing. But, there is essentially no significant change in mRNA expression between P14 and P21, the major period of hearing maturation.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 我们将继续研究消除哺乳动物耳蜗核的兴奋性突触输入后导致神经元死亡（或存活）的细胞间和细胞内信号传导途径。在当前赠款期间的一项主要倡议是使用现代遗传分析方法开始评估新生儿和成年动物对小鼠耳蜗核的传染性剥夺（关键时期）的差异反应的生物学机制。基因微阵列技术用于筛选筛选基因，这些基因在在外围剥夺的前48个小时后与在此关键时期内受到差异调控的易感性不同的时期在人工耳蜗核中差异化表达的基因。这些研究最有趣，最重要的发现之一是，在传入依赖性神经元生存期（P2-P11; P =产后）和传入之间的转变过程中发生了巨大变化。 独立生存（> p14），与听力的发作密切相对应。但是，p14和p21之间的mRNA表达基本上没有显着变化，这是听力成熟的主要时期。