IDENTIFICATION OF THE DROSOPHILA F-BOX PROTEIN PALLBEARER SUBSTRATE(S)

果蝇 F-BOX 蛋白 PALLBEARER 底物的鉴定

• 批准号: 8171444
• 负责人: Nathalie Claudine Franc
• 金额: $ 0.24万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171444
• 关键词: Age related macular degeneration; Apoptotic; Autoimmune Diseases; Blindness; Boxing; Cells; Co-Immunoprecipitations; Complex; Computer Retrieval of Information on Scientific Projects Database; Development; Drosophila genus; F-Box Proteins; Failure; Funding; Goals; Grant; Immune response; Immunity; Institution; Laboratories; Lupus; Mass Spectrum Analysis; Nerve Degeneration; Organism; Play; Proteins; Research; Research Personnel; Resolution; Resources; Role; Source; United States National Institutes of Health; novel; ubiquitin-protein ligase

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Apoptotic cell clearance is crucial to the development and the resolution of immune responses in all multicellular organisms. Failure to clear apoptotic cells can trigger autoimmune diseases such as Lupus, and neurodegeneration, such as age-related macular degeneration, the most frequent cause of blindness. We have identified a novel Drosophila F-Box protein that plays a role in apoptotic cell clearance (Silva et al, 2007. Immunity 27 (4): 585-96). This F-Box protein acts together with SkpA, Lin19 and Effete to form a SCF complex with E3 ligase activity. The goal of this project is to couple co-ImmunoPrecipitation and Mass Spectrometry to identify potential Pallbearer substrate(s), which will then be further characterized in our laboratory.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 凋亡细胞清除对于所有多细胞生物的免疫反应的发育和分辨率至关重要。无法清除凋亡细胞会触发自身免疫性疾病，例如狼疮和神经变性，例如与年龄相关的黄斑变性，这是失明的最常见原因。 我们已经确定了一种新型的果蝇F-box蛋白，该蛋白在凋亡细胞清除率中起作用（Silva等，2007。免疫27（4）：585-96）。该F-box蛋白与SKPA，LIN19和EFFETE一起起作用，形成具有E3连接酶活性的SCF复合物。该项目的目的是将共免疫沉淀和质谱法进行鉴定潜在的Pallbearer底物，然后在我们的实验室中进一步表征。