STRUCTURAL STUDY OF DISEASE RELATED FACTORS

疾病相关因素的结构研究

• 批准号: 8169274
• 负责人: Yong Xiong
• 金额: $ 3.49万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169274
• 关键词: Amyloid; Antiviral Agents; Archaea; Binding; Computer Retrieval of Information on Scientific Projects Database; Cytidine; Cytidine Deaminase; Deamination; Detection; Disease; Enzymes; Family; Funding; Genes; Grant; Institution; Link; Location; Positioning Attribute; Proteins; Research; Research Personnel; Resources; Source; Structure; Thioflavin T; Transfer RNA; United States National Institutes of Health; Uridine; Work; adenosine deaminase; stem

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We focus on various cytidine deaminases, including the antiviral protein APOBEC3G and adenosine deaminases acting on tRNA. All canonical transfer RNAs (tRNAs) have a uridine at position 8, involved in maintaining tRNA tertiary structure. However, the hyperthermophilic archaeon Methanopyrus kandleri harbors 30 (out of 34) tRNA genes with cytidine at position 8. We demonstrate C-to-U editing at this location in the tRNA's tertiary core, and present the crystal structure of a tRNA-specific cytidine deaminase, CDAT8, which has the cytidine deaminase domain linked to a tRNA-binding THUMP domain. CDAT8 is specific for C deamination at position 8, requires only the acceptor stem hairpin for activity, and belongs to a unique family within the "cytidine deaminaselike" superfamily. The presence of this C-to-U editing enzyme facilitates the proper folding and functionality of all M. kandleri tRNAs. We also work on the mechanism of amyloid detection by Thioflavin T.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 我们专注于各种胞苷脱氨酶，包括作用于tRNA的抗病毒蛋白APOBEC3G和腺苷脱氨酶。所有规范转移RNA（TRNA）在位置8处具有尿苷，涉及维持tRNA三级结构。然而，超育素甲虫Kandleri kandleri在位置8处具有30（34个）tRNA基因。 领域。 CDAT8针对位置8处的C脱氨酸特异性，仅需要受体茎发夹才能进行活动，并且属于“ cytidine deaminase”中的独特家族，例如”超家族。这种C-TO-U编辑酶的存在有助于所有坎德勒·托纳斯的适当折叠和功能。我们还致力于硫非类Thioflavin T的淀粉样蛋白检测机理。