LOW-POWER KA BAND BRIDGE FOR PULSED ENDOR APPLICATION

适用于脉冲 ENDOR 应用的低功耗 KA 波段电桥

• 批准号: 8172094
• 负责人: PETER P BORBAT
• 金额: $ 0.58万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172094
• 关键词: Area; Computer Retrieval of Information on Scientific Projects Database; Data Collection; Development; Electron Nuclear Double Resonance; Equipment; Funding; Grant; Institution; Knowledge; Ligands; Metalloproteins; Metals; Methods; Output; Phase; Physiologic pulse; Proteins; Reporting; Research; Research Personnel; Resolution; Resources; Source; Spin Labels; Structure; Time; United States National Institutes of Health; fundamental research; microwave electromagnetic radiation; programs

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Besides the folding of spin labeled proteins, the major fundamental research area of the Scholes lab is the application of ENDOR (electron-nuclear double resonance) to paramagnetic metalloproteins. Until now Scholes has used CW ENDOR methods. Pulsed ENDOR as opposed to CW ENDOR, would provide better resolution of small couplings that report on metal-ligand structure. The technical complexity of pulsed microwaves together with the ~$500,000 expense of commercial equipment have been the reasons why the Scholes lab does not do pulsed ENDOR. Recent developments of Peter Borbat in the Freed lab have made it clear that a pulsed Q-band bridge having moderate pulse output (1 to 5 W), appropriate timing circuits, phase shifting, pulse programming, and data collection can be made at ACERT for ~$50,000. The ability to do pulsed ENDOR will be a major technical advancement for the Scholes lab. The proximity of Albany and Cornell will make for easy transfer of information and knowledge.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 中心，不一定是研究者的机构。 除了自旋标记蛋白的折叠外，Scholes实验室的主要基础研究领域是ENDOR（电子核双共振）在顺磁性金属蛋白中的应用。到目前为止，斯科尔斯一直使用 CW ENDOR 方法。与 CW ENDOR 相比，脉冲 ENDOR 将为报告金属配体结构的小型耦合提供更好的分辨率。脉冲微波的技术复杂性以及大约 500,000 美元的商业设备费用是斯科尔斯实验室不进行脉冲 ENDOR 的原因。 Freed 实验室 Peter Borbat 的最新进展表明，可以在 ACERT 制造具有中等脉冲输出（1 至 5 W）、适当的定时电路、相移、脉冲编程和数据收集的脉冲 Q 波段电桥，用于〜50,000 美元。进行脉冲 ENDOR 的能力将是斯科尔斯实验室的一项重大技术进步。奥尔巴尼和康奈尔大学毗邻，便于信息和知识的传递。