SPECTROMETER FOR TIME-RESOLVED 2D-FT ESR

用于时间分辨 2D-FT ESR 的光谱仪

• 批准号: 8364110
• 负责人: PETER P BORBAT
• 金额: $ 0.16万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8364110
• 关键词: Development; Event; Frequencies; Funding; Grant; Kinetics; Lasers; Lighting; National Center for Research Resources; Physiologic pulse; Power Sources; Principal Investigator; Process; Research; Research Infrastructure; Resources; Series; Signal Transduction; Source; System; Technology; Time; United States National Institutes of Health; Update; Work; base; cost; microwave electromagnetic radiation; millisecond; nanosecond; operation; protein folding; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. A pulse FT ESR spectrometer capable of capturing transient events on nanosecond time scales and simultaneously recording slower Kinetics on microsecond to millisecond time scale has not been realized elsewhere. A series of technical developments at ACERT have enabled implementation of such a spectrometer. Such a spectrometer requires very short dead-times, high pulse-sequence repetition rates, a unique timing system capable of updating all pulse sequence parameters on every shot (in a few microseconds), and capturing and processing transient signals at such high rates. Also, the shortest dead-times and the highest sensitivity can be achieved at a higher working frequency. All these components have been developed at ACERT and are being integrated into a unique spectrometer. Recent acquisition by ACERT of a Bruker E500 CW ESR spectrometer made available a 12-inch resistive electromagnet for this project, thereby relieving to some extent the inconvenience of arranging several largely incompatible pulse ESR projects around a few available electromagnets. 2D-ELDOR experiments from X to Ku band will be built around this magnet. The existing electromagnet power supply allows this new spectrometer operation up to Ku band, where high quality 2D-ELDOR was demonstrated. Currently the operation is limited to X-band (based on 8 kW peak power TWTA), but operation at Ku band is also planned. Operation at Ka band is also planned, but has to be combined with PDS spectrometer. The spectrometer will be used with closed-loop flow system to study photo processes under pulse laser illumination and with rapid mixing system to study protein folding. Broadband 8-18 GHz (in five bands) microwave bridge, built in early 2000's, will be used in a modern modified version. Time-resolved operation will be entirely based on a dedicated versatile pulse-programmer with real-time control, ACERT-BN2 or its successor.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的首席研究员可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 脉冲 FT ESR 光谱仪能够捕获纳秒时间尺度的瞬态事件并同时记录较慢的速度 微秒到毫秒时间尺度的动力学尚未在其他地方实现。 ACERT 的一系列技术开发使得这种光谱仪得以实施。这种光谱仪需要非常短的死区时间、高脉冲序列重复率、独特的定时系统，能够在每次发射时（几微秒内）更新所有脉冲序列参数，并以如此高的速率捕获和处理瞬态信号。此外，可以在更高的工作频率下实现最短的死区时间和最高的灵敏度。所有这些组件均由 ACERT 开发，并正在集成到独特的光谱仪中。 ACERT 最近购买了布鲁克 E500 CW ESR 光谱仪，为该项目提供了 12 英寸电阻电磁体，从而在一定程度上缓解了围绕几个可用电磁体安排几个基本不兼容的脉冲 ESR 项目的不便。 从 X 到 Ku 波段的 2D-ELDOR 实验将围绕该磁体进行。现有的电磁体电源允许这种新的光谱仪运行到 Ku 波段，在该波段展示了高质量的 2D-ELDOR。 目前，该操作仅限于 X 频段（基于 8 kW 峰值功率 TWTA），但也计划在 Ku 频段操作。还计划在 Ka 波段进行操作，但必须与 PDS 光谱仪结合使用。该光谱仪将与闭环流动系统一起使用，以研究脉冲激光照射下的光过程，并与快速混合系统一起使用，以研究蛋白质折叠。宽带 8-18 GHz（五个频段）微波桥建于 2000 年代初，将用于现代改进版本。时间分辨操作将完全基于具有实时控制功能的专用多功能脉冲编程器 ACERT-BN2 或其后续产品。