ASSIGNMENT OF POSTTRANSLATIONAL MODIFICATIONS IN STREPTOLYSIN-S ANALOGUE

链球菌溶血素-S 类似物中翻译后修饰的分配

• 批准号: 8168991
• 负责人: JACK E DIXON
• 金额: $ 0.19万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168991
• 关键词: Bacteria; Computer Retrieval of Information on Scientific Projects Database; Cysteine; Cytolysins; Funding; Grant; Institution; Maps; Mass Spectrum Analysis; Oxazoles; Peptides; Phenotype; Play; Post-Translational Protein Processing; Research; Research Personnel; Resources; Role; Serine; Side; Source; Streptococcus pyogenes; Streptolysins; Structure; Thiazoles; Threonine; United States National Institutes of Health; Virulence; analog; perforin

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Streptolysin S (SLS) is a potent cytolysin produced by Streptococcus Pyogenes. SLS plays a key role in virulence and is responsible for the b-hemolytic phenotype of these bacteria. To date, the structure of SLS has not been identified. Previous studies have shown that SLS is a heavily posttranslationally modified ribosomally encoded peptide. In order for the peptide to become cytolytic the side chains of one or more of the cysteine, serine or threonines are heterocyclized into five membered thiazole or oxazole rings. Repeated attempts to identify and map these posttranslational modifications by mass spectrometry have failed. This study will use NMR to do a sequential assignment by triple resonance on a recombinantly produced SLS analogue.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 链球菌溶血素 S (SLS) 是一种由化脓性链球菌产生的强效溶细胞素。 SLS 在毒力中发挥着关键作用，并导致这些细菌的 b 溶血表型。 迄今为止，SLS的结构尚未确定。 先前的研究表明，SLS 是一种经过大量翻译后修饰的核糖体编码肽。 为了使肽具有溶细胞性，半胱氨酸、丝氨酸或苏氨酸中的一个或多个的侧链被杂环化成五元噻唑或恶唑环。 通过质谱法识别和绘制这些翻译后修饰的多次尝试都失败了。 本研究将使用 NMR 通过三重共振对重组生产的 SLS 类似物进行顺序分配。