CELL TISSUE AND IMAGING CORE

细胞组织和成像核心

基本信息

批准号：
7778740
负责人：
Sarah F Hamm-Alvarez
金额：
$ 28.82万
依托单位：
UNIVERSITY OF SOUTHERN CALIFORNIA
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-03-01 至 2015-02-28
项目状态：
已结题

项目摘要

During the past funding period, the Cell Culture Core has continued to provide isolation of mouse and rat hepatocytes from normal and diseased livers, cell line banking, and distribution of human hepatocytes. A separately funded (through NIAAA beginning in mid-2001) Non-parenchymal Liver Cell Core provides freshly isolated and cultured normal or diseased rat Kupffer cells, hepatic stellate cells, and sinusoidal endothelial cells. Effective 9/1/04, the Cell Culture Core stopped preparing human hepatocytes but instead distributes human hepatocytes prepared by a commercial source (CellzDirect, Tucson, Az). This is because an agreement was made between the USC liver surgeons and CellzDirect, which supports the liver tissue repository at USC and provides human hepatocytes free of charge to Liver Center investigators. The Cell Culture Core has continued to provide a vital service to the Center members and has remained near steady state from the previoijs to the current grant cycle in terms of usage (number of preps, investigators and grants) and productivity (number of publications). Sixteen members and affiliated members used the Cell Culture Core from 1998 to 2003 with 47 publications. In the current cycle, 2003 to 2008, 18 full members used the Core and generated 50 publications. In particular, the Cell Culture Core has been instrumental in assisting young investigators in establishing their independent research programs (e.g. H.P. Yang, D. Han, C. Ji and Z.X. Liu).

在过去的资金期间，细胞培养核心继续提供小鼠的隔离和来自正常和患病肝脏的大鼠肝细胞，细胞系库以及人肝细胞的分布。一个分别资助（从2001年中开始）（通过NIAAA）非核肝细胞核提供了新鲜分离和培养的正常或患病的大鼠库普弗细胞，肝星状细胞和正弦内皮细胞细胞。有效9/1/04，细胞培养核心停止准备人肝细胞，而是分布由商业来源制备的人肝细胞（CellzDirect，Tucson，AZ）。这是因为在支持肝组织的USC肝外科医生和CellzDirect之间达成了一致 USC的存储库，并向肝脏中心的调查人员免费提供人类肝细胞。细胞培养核心继续为中心成员提供重要服务，并拥有就用法而言，从previoijs到当前赠款周期保持稳定状态（预备次数，调查人员和赠款）和生产力（出版物数量）。 16名成员和附属成员从1998年到2003年，使用了47个出版物。在当前周期，2003年至2008年，18个成员使用了核心，并生成了50个出版物。特别是，细胞培养核心一直是为协助年轻研究人员建立独立研究计划而发挥了作用（例如H.P. Yang，D。Han，C。Ji和Z.X.刘）。