LSUHSC COBRE: CORE 6: PROTEOMICS

LSUHSC COBRE：核心 6：蛋白质组学

• 批准号: 8168432
• 负责人: CHAU-WEN CHOU
• 金额: $ 0.15万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168432
• 关键词: Cells; Centers of Research Excellence; Computer Retrieval of Information on Scientific Projects Database; Computer software; Consult; Core Facility; Electrophoresis; Fluorescence; Funding; Gel; Generations; Grant; Healthcare; Housing; Human Resources; Image; Image Analysis; Institution; Label; Laboratories; Liquid Chromatography; Louisiana; Mass Spectrum Analysis; Proteins; Proteomics; Research; Research Personnel; Resources; Running; Sampling; Services; Source; Spottings; System; Tissues; Two-Dimensional Gel Electrophoresis; United States National Institutes of Health; Variant; anticancer research; base; design; experience; gel electrophoresis; mass spectrometer; medical schools; research study; square foot

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The LSUHSC Proteomics Core Facility, supported by this COBRE, the LSUHSC School of Medicine, the Louisiana Cancer Research Consortium, offers proteomic services and resources to investigators both within and outside of LSUHSC. The facility personnel consult in the design and implementation of experiments for the advancement of investigators' projects. The main laboratory occupies ~1300 sq. ft. of laboratory space to house several advanced mass spectrometers, 2-Diminsional gel electrophoresis and 2-Diminsional Liquid Chromatography. An additional 500 sq. ft. of laboratory space is dedicated to 2-Dimensional gel electrophoresis. The Proteomics Core Facility is highly experienced in protein extractions from cells and tissues for 2DGE. Currently, the new generation fluorescence-tag based 2DGE, different gel electrophoresis (DiGE), is the choice of the Facility. DiGE eliminates gel variations by running differentially tagged control and treated samples in the same gel. The uniquely labeled protein samples are separated using an IPGphor IEF and a Dalt6 PAGE cell from GE Healthcare. Following electrophoresis, a GE 9400 Typhoon scanner image is analyzed with DeCyder image analysis software. This software provides investigators with statistically accurate and sensitive protein quantification. A GE Ettan spot handling workstation cuts and digests gel spots automatically for sequential protein identification by mass spectrometry. In addition, traditional 2-dimensional gel electrophoresis utilizing the Bio-Rad system (PDQuest software, ProteomeWorks spot cutter and GS800 scanner from Bio-Rad and MultiProbe spot handling system from Perkin-Elmer) is also offered for investigators when needed.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 LSUHSC 蛋白质组学核心设施在 COBRE、LSUHSC 医学院、路易斯安那州癌症研究联盟的支持下，为 LSUHSC 内外的研究人员提供蛋白质组学服务和资源。 设施人员在实验的设计和实施方面提供咨询，以推进研究人员的项目。 主实验室占地约 1300 平方英尺的实验室空间，可容纳数台先进的质谱仪、二维凝胶电泳仪和二维液相色谱仪。 另外 500 平方英尺的实验室空间专门用于二维凝胶电泳。 蛋白质组学核心设施在从细胞和组织中提取蛋白质用于 2DGE 方面拥有丰富的经验。 目前，该设施选择基于新一代荧光标签的 2DGE、不同凝胶电泳 (DiGE)。 DiGE 通过在同一凝胶中运行差异标记的对照和处理样品来消除凝胶变化。 使用 GE Healthcare 的 IPGphor IEF 和 Dalt6 PAGE 细胞分离独特标记的蛋白质样品。电泳后，使用 DeCyder 图像分析软件对 GE 9400 Typhoon 扫描仪图像进行分析。该软件为研究人员提供统计上准确且灵敏的蛋白质定量。 GE Ettan 斑点处理工作站自动切割和消化凝胶斑点，以便通过质谱法进行连续蛋白质鉴定。此外，在需要时，还可以为研究人员提供利用 Bio-Rad 系统（Bio-Rad 的 PDQuest 软件、ProteomeWorks 斑点切割器和 GS800 扫描仪以及 Perkin-Elmer 的 MultiProbe 斑点处理系统）的传统二维凝胶电泳。